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- Publisher Website: 10.1128/JVI.78.10.5244-5257.2004
- Scopus: eid_2-s2.0-2342569732
- PMID: 15113906
- WOS: WOS:000221212100032
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Article: CD4+ T Cells from CD4C/HIVNef Transgenic Mice Show Enhanced Activation In Vivo with Impaired Proliferation In Vitro but Are Dispensable for the Development of a Severe AIDS-Like Organ Disease
Title | CD4<sup>+</sup> T Cells from CD4C/HIV<sup>Nef</sup> Transgenic Mice Show Enhanced Activation In Vivo with Impaired Proliferation In Vitro but Are Dispensable for the Development of a Severe AIDS-Like Organ Disease |
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Authors | |
Issue Date | 2004 |
Citation | Journal of Virology, 2004, v. 78, n. 10, p. 5244-5257 How to Cite? |
Abstract | The cellular and molecular mechanisms of dysfunction and depletion of CD4+ T lymphocytes over the course of human immunodeficiency virus type 1 (HIV-1) infection are still incompletely understood, but chronic immune activation is thought to play an important role in disease progression. We studied CD4+ T-cell biology in CD4C/HIV transgenic (Tg) mice, in which Nef expression is sufficient to induce a severe AIDS-like disease including a preferential decrease of CD4+ T cells. We show here that Nef-expressing Tg CD4+ T cells exhibit an activated/memory-like phenotype which appears to be independent of antigenic stimulation, as documented in experiments involving breeding with AD10 TcR Tg mice. In addition, in vivo bromodeoxyuridine incorporation showed that a larger proportion of Tg than non-Tg CD4+ T cells entered the S phase. However, in vitro, Tg CD4+ T cells were found to have a very limited capacity to divide in response to stimulation with anti-CD3 and anti-CD28 or in allogeneic mixed leukocyte reactions. Interestingly, despite these observations, the deletion of Tg CD4+ T cells had little impact on the development of other AIDS-like organ phenotypes. Thus, the Nef-induced chronic activation of CD4 + T cells may exhaust the T-cell pool and may contribute to the thymic atrophy and the low number of CD4+ T cells observed in these Tg mice. |
Persistent Identifier | http://hdl.handle.net/10722/291742 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.378 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Weng, Xiaoduan | - |
dc.contributor.author | Priceputu, Elena | - |
dc.contributor.author | Chrobak, Pavel | - |
dc.contributor.author | Poudrier, Johanne | - |
dc.contributor.author | Kay, Denis G. | - |
dc.contributor.author | Hanna, Zaher | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Jolicoeur, Paul | - |
dc.date.accessioned | 2020-11-17T14:55:01Z | - |
dc.date.available | 2020-11-17T14:55:01Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Journal of Virology, 2004, v. 78, n. 10, p. 5244-5257 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10722/291742 | - |
dc.description.abstract | The cellular and molecular mechanisms of dysfunction and depletion of CD4+ T lymphocytes over the course of human immunodeficiency virus type 1 (HIV-1) infection are still incompletely understood, but chronic immune activation is thought to play an important role in disease progression. We studied CD4+ T-cell biology in CD4C/HIV transgenic (Tg) mice, in which Nef expression is sufficient to induce a severe AIDS-like disease including a preferential decrease of CD4+ T cells. We show here that Nef-expressing Tg CD4+ T cells exhibit an activated/memory-like phenotype which appears to be independent of antigenic stimulation, as documented in experiments involving breeding with AD10 TcR Tg mice. In addition, in vivo bromodeoxyuridine incorporation showed that a larger proportion of Tg than non-Tg CD4+ T cells entered the S phase. However, in vitro, Tg CD4+ T cells were found to have a very limited capacity to divide in response to stimulation with anti-CD3 and anti-CD28 or in allogeneic mixed leukocyte reactions. Interestingly, despite these observations, the deletion of Tg CD4+ T cells had little impact on the development of other AIDS-like organ phenotypes. Thus, the Nef-induced chronic activation of CD4 + T cells may exhaust the T-cell pool and may contribute to the thymic atrophy and the low number of CD4+ T cells observed in these Tg mice. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Virology | - |
dc.title | CD4<sup>+</sup> T Cells from CD4C/HIV<sup>Nef</sup> Transgenic Mice Show Enhanced Activation In Vivo with Impaired Proliferation In Vitro but Are Dispensable for the Development of a Severe AIDS-Like Organ Disease | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JVI.78.10.5244-5257.2004 | - |
dc.identifier.pmid | 15113906 | - |
dc.identifier.pmcid | PMC400335 | - |
dc.identifier.scopus | eid_2-s2.0-2342569732 | - |
dc.identifier.volume | 78 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 5244 | - |
dc.identifier.epage | 5257 | - |
dc.identifier.isi | WOS:000221212100032 | - |
dc.identifier.issnl | 0022-538X | - |