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Article: Comparison of plasma β-globin DNA and S-100 protein concentrations in acute stroke

TitleComparison of plasma β-globin DNA and S-100 protein concentrations in acute stroke
Authors
KeywordsS100 protein
Plasma β-globin DNA
Stroke
Prognosis
Emergency department
Issue Date2007
Citation
Clinica Chimica Acta, 2007, v. 376, n. 1-2, p. 190-196 How to Cite?
AbstractBackground: This study aimed to compare changes in plasma β-globin DNA and serum S100 protein to diagnose stroke and for predicting mortality and morbidity. Methods: Patients with stroke-like symptoms presenting to the emergency department of a Hong Kong hospital were recruited. Plasma DNA was analyzed for the β-globin gene with fluorescent-based PCR. S100 concentrations were determined using ELISA. Primary outcomes were diagnosis of stroke, mortality, and modified Rankin Score (mRS) after 6 months. Results: One hundred ninety-seven consecutive patients recruited, 118 (60%) ischemic stroke, 35 (18%) hemorrhage and 44 (22%) with no acute neuroimaging changes. Serum S100 and plasma DNA were increased in 126 (p < 0.0010) and 36 (p = 0.21) stroke patients respectively vs. controls. Median plasma DNA was higher in hemorrhagic stroke than those without (1725 vs. 1050 kilogenome-equivalents/l, p = 0.0104). Median plasma DNA was higher in mRS > 2 vs. mRS ≤ 2 (1350 vs. 1025, p = 0.0103), and higher in non-survivors vs. survivors (1625 vs. 1050, p = 0.0070). Median serum S100 higher in mRS > 2 patients vs. mRS ≤ 2 (0.152 vs. 0.131 μg/l, p = 0.0003). The odds ratio (OR) of discriminating hemorrhagic from non-hemorrhagic stroke with DNA was 4.24 (95% CI 1.88-9.56); S100 and DNA together give an OR of 16.55. Conclusion: For stroke diagnosis, S100 performs better than DNA; DNA is a better marker for hemorrhage. For diagnosis of hemorrhagic stroke, combined S100 and DNA performs better than either alone. Plasma DNA and serum S100 predict morbidity and mortality in stroke. © 2006 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/291770
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 1.016
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRainer, Timothy H.-
dc.contributor.authorWong, Ka Sing-
dc.contributor.authorLam, Wynnie-
dc.contributor.authorLam, Nicole Y.L.-
dc.contributor.authorGraham, Colin A.-
dc.contributor.authorLo, Y. M.Dennis-
dc.date.accessioned2020-11-17T14:55:04Z-
dc.date.available2020-11-17T14:55:04Z-
dc.date.issued2007-
dc.identifier.citationClinica Chimica Acta, 2007, v. 376, n. 1-2, p. 190-196-
dc.identifier.issn0009-8981-
dc.identifier.urihttp://hdl.handle.net/10722/291770-
dc.description.abstractBackground: This study aimed to compare changes in plasma β-globin DNA and serum S100 protein to diagnose stroke and for predicting mortality and morbidity. Methods: Patients with stroke-like symptoms presenting to the emergency department of a Hong Kong hospital were recruited. Plasma DNA was analyzed for the β-globin gene with fluorescent-based PCR. S100 concentrations were determined using ELISA. Primary outcomes were diagnosis of stroke, mortality, and modified Rankin Score (mRS) after 6 months. Results: One hundred ninety-seven consecutive patients recruited, 118 (60%) ischemic stroke, 35 (18%) hemorrhage and 44 (22%) with no acute neuroimaging changes. Serum S100 and plasma DNA were increased in 126 (p < 0.0010) and 36 (p = 0.21) stroke patients respectively vs. controls. Median plasma DNA was higher in hemorrhagic stroke than those without (1725 vs. 1050 kilogenome-equivalents/l, p = 0.0104). Median plasma DNA was higher in mRS > 2 vs. mRS ≤ 2 (1350 vs. 1025, p = 0.0103), and higher in non-survivors vs. survivors (1625 vs. 1050, p = 0.0070). Median serum S100 higher in mRS > 2 patients vs. mRS ≤ 2 (0.152 vs. 0.131 μg/l, p = 0.0003). The odds ratio (OR) of discriminating hemorrhagic from non-hemorrhagic stroke with DNA was 4.24 (95% CI 1.88-9.56); S100 and DNA together give an OR of 16.55. Conclusion: For stroke diagnosis, S100 performs better than DNA; DNA is a better marker for hemorrhage. For diagnosis of hemorrhagic stroke, combined S100 and DNA performs better than either alone. Plasma DNA and serum S100 predict morbidity and mortality in stroke. © 2006 Elsevier B.V. All rights reserved.-
dc.languageeng-
dc.relation.ispartofClinica Chimica Acta-
dc.subjectS100 protein-
dc.subjectPlasma β-globin DNA-
dc.subjectStroke-
dc.subjectPrognosis-
dc.subjectEmergency department-
dc.titleComparison of plasma β-globin DNA and S-100 protein concentrations in acute stroke-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cca.2006.08.025-
dc.identifier.pmid17027951-
dc.identifier.scopuseid_2-s2.0-33845397549-
dc.identifier.volume376-
dc.identifier.issue1-2-
dc.identifier.spage190-
dc.identifier.epage196-
dc.identifier.isiWOS:000243585300031-
dc.identifier.issnl0009-8981-

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