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Article: Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice

TitlePten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice
Authors
Issue Date2007
Citation
Journal of Clinical Investigation, 2007, v. 117, n. 10, p. 2929-2940 How to Cite?
AbstractPTEN is a tumor suppressor gene mutated in many human cancers. We generated a bronchioalveolar epithelium-specific null mutation of Pten in mice [SP-C-rtTA/(tetO)7-Cre/Ptenflox/flox (SOPten flox/flox) mice] that was under the control of doxycycline. Ninety percent of SOPtenflox/flox mice that received doxycycline in utero [SOPtenflox/flox(E10-16) mice] died of hypoxia soon after birth. Surviving SOPtenflox/flox(E10-16) mice and mice that received doxycycline postnatally [SOPtenflox/flox(P21-27) mice] developed spontaneous lung adenocarcinomas. Urethane treatment accelerated number and size of lung tumors developing in SOPtenflox/flox mice of both ages. Histological and biochemical examinations of the lungs of SOPten flox/flox(E10-16) mice revealed hyperplasia of bronchioalveolar epithelial cells and myofibroblast precursors, enlarged alveolar epithelial cells, and impaired production of surfactant proteins. Numbers of bronchioalveolar stem cells (BASCs), putative initiators of lung adenocarcinomas, were increased. Lungs of SOPtenflox/flox(E10-16) mice showed increased expression of Spry2, which inhibits the maturation of alveolar epithelial cells. Levels of Akt, c-Myc, Bcl-2, and Shh were also elevated in SOPtenflox/flox(E10-16) and SOPten flox/flox(P21-27) lungs. Furthermore, K-ras was frequently mutated in adenocarcinomas observed in SOPtenflox/flox(P21-27) lungs. These results indicate that Pten is essential for both normal lung morphogenesis and the prevention of lung carcinogenesis, possibly because this tumor suppressor is required for BASC homeostasis.
Persistent Identifierhttp://hdl.handle.net/10722/291790
ISSN
2023 Impact Factor: 13.3
2023 SCImago Journal Rankings: 4.833
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYanagi, Shigehisa-
dc.contributor.authorKishimoto, Hiroyuki-
dc.contributor.authorKawahara, Kohichi-
dc.contributor.authorSasaki, Takehiko-
dc.contributor.authorSasaki, Masato-
dc.contributor.authorNishio, Miki-
dc.contributor.authorYajima, Nobuyuki-
dc.contributor.authorHamada, Koichi-
dc.contributor.authorHorie, Yasuo-
dc.contributor.authorKubo, Hiroshi-
dc.contributor.authorWhitsett, Jeffrey A.-
dc.contributor.authorMak, Tak Wah-
dc.contributor.authorNakano, Toru-
dc.contributor.authorNakazato, Masamitsu-
dc.contributor.authorSuzuki, Akira-
dc.date.accessioned2020-11-17T14:55:07Z-
dc.date.available2020-11-17T14:55:07Z-
dc.date.issued2007-
dc.identifier.citationJournal of Clinical Investigation, 2007, v. 117, n. 10, p. 2929-2940-
dc.identifier.issn0021-9738-
dc.identifier.urihttp://hdl.handle.net/10722/291790-
dc.description.abstractPTEN is a tumor suppressor gene mutated in many human cancers. We generated a bronchioalveolar epithelium-specific null mutation of Pten in mice [SP-C-rtTA/(tetO)7-Cre/Ptenflox/flox (SOPten flox/flox) mice] that was under the control of doxycycline. Ninety percent of SOPtenflox/flox mice that received doxycycline in utero [SOPtenflox/flox(E10-16) mice] died of hypoxia soon after birth. Surviving SOPtenflox/flox(E10-16) mice and mice that received doxycycline postnatally [SOPtenflox/flox(P21-27) mice] developed spontaneous lung adenocarcinomas. Urethane treatment accelerated number and size of lung tumors developing in SOPtenflox/flox mice of both ages. Histological and biochemical examinations of the lungs of SOPten flox/flox(E10-16) mice revealed hyperplasia of bronchioalveolar epithelial cells and myofibroblast precursors, enlarged alveolar epithelial cells, and impaired production of surfactant proteins. Numbers of bronchioalveolar stem cells (BASCs), putative initiators of lung adenocarcinomas, were increased. Lungs of SOPtenflox/flox(E10-16) mice showed increased expression of Spry2, which inhibits the maturation of alveolar epithelial cells. Levels of Akt, c-Myc, Bcl-2, and Shh were also elevated in SOPtenflox/flox(E10-16) and SOPten flox/flox(P21-27) lungs. Furthermore, K-ras was frequently mutated in adenocarcinomas observed in SOPtenflox/flox(P21-27) lungs. These results indicate that Pten is essential for both normal lung morphogenesis and the prevention of lung carcinogenesis, possibly because this tumor suppressor is required for BASC homeostasis.-
dc.languageeng-
dc.relation.ispartofJournal of Clinical Investigation-
dc.titlePten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1172/JCI31854-
dc.identifier.pmid17909629-
dc.identifier.pmcidPMC1994617-
dc.identifier.scopuseid_2-s2.0-34948850396-
dc.identifier.volume117-
dc.identifier.issue10-
dc.identifier.spage2929-
dc.identifier.epage2940-
dc.identifier.eissn1558-8238-
dc.identifier.isiWOS:000249894400024-
dc.identifier.issnl0021-9738-

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