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- Publisher Website: 10.1182/blood-2010-06-291062
- Scopus: eid_2-s2.0-78149433577
- PMID: 20660792
- WOS: WOS:000284110400019
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Article: Targeted deletion of the tachykinin 4 gene (TAC4-/-) influences the early stages of B lymphocyte development
Title | Targeted deletion of the tachykinin 4 gene (TAC4<sup>-/-</sup>) influences the early stages of B lymphocyte development |
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Authors | |
Issue Date | 2010 |
Citation | Blood, 2010, v. 116, n. 19, p. 3792-3801 How to Cite? |
Abstract | Hemokinin-1 (HK-1), encoded by the TAC4 gene, is a tachykinin peptide that is predominantly expressed in non-neuronal cells, such as immune cells. We have disrupted the mouse TAC4 gene to obtain a better understanding of the actions of HK-1 during hematopoiesis. We demonstrate here that TAC4-/- mice exhibit an increase of CD19+CD117+HSA+BP.1 - "fraction B" pro-B cells in the bone marrow, whereas pre-B, immature, and mature B cells are within the normal range. We show that in vitro cultures derived from TAC4-/- bone marrow, sorted "fraction B" pro-B cells or purified long-term reconstituting stem cells, contain significantly higher numbers of pro-B cells compared with controls, suggesting an inhibitory role for HK-1 on developing B cells. Supporting this idea, we show that addition of HK-1 to cultures established from long-term reconstituting stem cells and the newly described intermediate-term reconstituting stem cells leads to a significant decrease of de novo generated proB cells. Based on our studies, we postulate that HK-1 plays an inhibitory role in hematopoiesis, and we hypothesize that it may be part of the bone marrow microenvironment that supports and regulates the proliferation and differentiation of hematopoietic cells. © 2010 by The American Society of Hematology. |
Persistent Identifier | http://hdl.handle.net/10722/291995 |
ISSN | 2023 Impact Factor: 21.0 2023 SCImago Journal Rankings: 5.272 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Berger, Alexandra | - |
dc.contributor.author | Benveniste, Patricia | - |
dc.contributor.author | Corfe, Steven A. | - |
dc.contributor.author | Tran, Anne H. | - |
dc.contributor.author | Barbara, Mary | - |
dc.contributor.author | Wakeham, Andrew | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Iscove, Norman N. | - |
dc.contributor.author | Paige, Christopher J. | - |
dc.date.accessioned | 2020-11-17T14:55:33Z | - |
dc.date.available | 2020-11-17T14:55:33Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Blood, 2010, v. 116, n. 19, p. 3792-3801 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | http://hdl.handle.net/10722/291995 | - |
dc.description.abstract | Hemokinin-1 (HK-1), encoded by the TAC4 gene, is a tachykinin peptide that is predominantly expressed in non-neuronal cells, such as immune cells. We have disrupted the mouse TAC4 gene to obtain a better understanding of the actions of HK-1 during hematopoiesis. We demonstrate here that TAC4-/- mice exhibit an increase of CD19+CD117+HSA+BP.1 - "fraction B" pro-B cells in the bone marrow, whereas pre-B, immature, and mature B cells are within the normal range. We show that in vitro cultures derived from TAC4-/- bone marrow, sorted "fraction B" pro-B cells or purified long-term reconstituting stem cells, contain significantly higher numbers of pro-B cells compared with controls, suggesting an inhibitory role for HK-1 on developing B cells. Supporting this idea, we show that addition of HK-1 to cultures established from long-term reconstituting stem cells and the newly described intermediate-term reconstituting stem cells leads to a significant decrease of de novo generated proB cells. Based on our studies, we postulate that HK-1 plays an inhibitory role in hematopoiesis, and we hypothesize that it may be part of the bone marrow microenvironment that supports and regulates the proliferation and differentiation of hematopoietic cells. © 2010 by The American Society of Hematology. | - |
dc.language | eng | - |
dc.relation.ispartof | Blood | - |
dc.title | Targeted deletion of the tachykinin 4 gene (TAC4<sup>-/-</sup>) influences the early stages of B lymphocyte development | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1182/blood-2010-06-291062 | - |
dc.identifier.pmid | 20660792 | - |
dc.identifier.scopus | eid_2-s2.0-78149433577 | - |
dc.identifier.volume | 116 | - |
dc.identifier.issue | 19 | - |
dc.identifier.spage | 3792 | - |
dc.identifier.epage | 3801 | - |
dc.identifier.eissn | 1528-0020 | - |
dc.identifier.isi | WOS:000284110400019 | - |
dc.identifier.issnl | 0006-4971 | - |