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Article: TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells

TitleTREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells
Authors
KeywordsTriggering expressed on myeloid cells-1
Group 3 innate lymphoid cells
Inflammatory macrophages
IL-22
Colitis
Issue Date2019
Citation
Journal of Biomedical Science, 2019, v. 26, n. 1, article no. 46 How to Cite?
AbstractBackground: Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses. To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse. Methods: Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS). Colonic lamina propria immune cell composition and cytokines were analyzed. An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels. Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity. Results: In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity. ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS. Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice. Conclusions: TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.
Persistent Identifierhttp://hdl.handle.net/10722/292118
ISSN
2020 Impact Factor: 8.41
2020 SCImago Journal Rankings: 2.182
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, Fu Chen-
dc.contributor.authorChiu, Po Yuan-
dc.contributor.authorChen, Yun-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorChen, Nien Jung-
dc.date.accessioned2020-11-17T14:55:48Z-
dc.date.available2020-11-17T14:55:48Z-
dc.date.issued2019-
dc.identifier.citationJournal of Biomedical Science, 2019, v. 26, n. 1, article no. 46-
dc.identifier.issn1021-7770-
dc.identifier.urihttp://hdl.handle.net/10722/292118-
dc.description.abstractBackground: Triggering receptor expressed on myeloid cells-1 (TREM-1) is highly expressed on macrophages in inflamed intestines and reportedly promotes inflammatory bowel disease (IBD) by augmenting pro-inflammatory responses. To study the mechanism mediated by TREM-1 on macrophages, we generated an independent TREM-1 deficient mouse. Methods: Acute colitis was induced in C57BL/6 and TREM-1-deficient mice by the administration of dextran sodium sulfate (DSS). Colonic lamina propria immune cell composition and cytokines were analyzed. An innate lymphoid cell (ILC) co-culture experiment with macrophages was used to analyze IL-22 levels. Exogenous IL-22 and TREM-1-expressing macrophages were supplied to TREM-1-deficient mice for examining their effects on intestinal barrier integrity. Results: In inflamed colons, TREM-1 loss compromised the activation of ILC3 and their production of IL-22, which is required for intestinal barrier integrity. ILC3-mediated IL-22 production depends on IL-1β secreted by M1-polarized macrophages, and we found that TREM-1 deficiency results in a decreased number of IL-1β producing-M1 macrophages in colons exposed to DSS. Accordingly, DSS-mediated damage was ameliorated by supplying exogenous IL-22 and TREM-1-expressing macrophages to TREM-1-deficient mice. Conclusions: TREM-1 plays a crucial role in regulating IL-22 production by ILC3 through modulating M1-macrophage polarization during DSS-induced acute colitis.-
dc.languageeng-
dc.relation.ispartofJournal of Biomedical Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectTriggering expressed on myeloid cells-1-
dc.subjectGroup 3 innate lymphoid cells-
dc.subjectInflammatory macrophages-
dc.subjectIL-22-
dc.subjectColitis-
dc.titleTREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s12929-019-0539-4-
dc.identifier.pmid31189465-
dc.identifier.pmcidPMC6560756-
dc.identifier.scopuseid_2-s2.0-85068165201-
dc.identifier.volume26-
dc.identifier.issue1-
dc.identifier.spagearticle no. 46-
dc.identifier.epagearticle no. 46-
dc.identifier.eissn1423-0127-
dc.identifier.isiWOS:000471272700001-
dc.identifier.issnl1021-7770-

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