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Article: Cross-species amplification of microsatellite loci for nine-spined stickleback Pungitius pungitius

TitleCross-species amplification of microsatellite loci for nine-spined stickleback Pungitius pungitius
Authors
Issue Date2007
Citation
Annales Zoologici Fennici, 2007, v. 44, n. 3, p. 218-224 How to Cite?
AbstractMicrosatellite loci of nine-spined stickleback (Pungitius pungitius) were optimized using primers originally designed for the three-spined stickleback (Gasterosteus aculeatus). Of the 57 three-spined stickleback loci tested, only 18 loci (32%) amplified a specific PCR product in the nine-spined stickleback. Further analysis for two Fennoscandian populations revealed 11 polymorphic and six monomorphic loci. The eleven polymorphic markers were optimized into two ready-to-go genotyping panels to facilitate genotyping applications, and these markers should prove useful for population genetic studies in the nine-spined stickleback. Comparison of polymorphism in the 11 loci between three- and nine-spined sticklebacks collected from the same two (lake or sea) localities revealed significantly lower polymorphism in the nine- than in the three-spined sticklebacks, and in the lake than in the sea populations of both species. Moreover, loss of polymorphism in the lake population was especially pronounced for the nine-spined stickleback (target species) as compared to the three-spined stickleback (source species). This suggests that the success of cross-species amplification may, in addition to well-known effects of e.g. species evolutionary divergence, depend on population history. © Finnish Zoological and Botanical Publishing Board 2007.
Persistent Identifierhttp://hdl.handle.net/10722/292196
ISSN
2023 Impact Factor: 0.9
2023 SCImago Journal Rankings: 0.310
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMäkinen, Hannu S.-
dc.contributor.authorVälimäki, Kaisa-
dc.contributor.authorMerilä, Juha-
dc.date.accessioned2020-11-17T14:55:58Z-
dc.date.available2020-11-17T14:55:58Z-
dc.date.issued2007-
dc.identifier.citationAnnales Zoologici Fennici, 2007, v. 44, n. 3, p. 218-224-
dc.identifier.issn0003-455X-
dc.identifier.urihttp://hdl.handle.net/10722/292196-
dc.description.abstractMicrosatellite loci of nine-spined stickleback (Pungitius pungitius) were optimized using primers originally designed for the three-spined stickleback (Gasterosteus aculeatus). Of the 57 three-spined stickleback loci tested, only 18 loci (32%) amplified a specific PCR product in the nine-spined stickleback. Further analysis for two Fennoscandian populations revealed 11 polymorphic and six monomorphic loci. The eleven polymorphic markers were optimized into two ready-to-go genotyping panels to facilitate genotyping applications, and these markers should prove useful for population genetic studies in the nine-spined stickleback. Comparison of polymorphism in the 11 loci between three- and nine-spined sticklebacks collected from the same two (lake or sea) localities revealed significantly lower polymorphism in the nine- than in the three-spined sticklebacks, and in the lake than in the sea populations of both species. Moreover, loss of polymorphism in the lake population was especially pronounced for the nine-spined stickleback (target species) as compared to the three-spined stickleback (source species). This suggests that the success of cross-species amplification may, in addition to well-known effects of e.g. species evolutionary divergence, depend on population history. © Finnish Zoological and Botanical Publishing Board 2007.-
dc.languageeng-
dc.relation.ispartofAnnales Zoologici Fennici-
dc.titleCross-species amplification of microsatellite loci for nine-spined stickleback Pungitius pungitius-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.scopuseid_2-s2.0-34447566096-
dc.identifier.volume44-
dc.identifier.issue3-
dc.identifier.spage218-
dc.identifier.epage224-
dc.identifier.isiWOS:000248181600006-
dc.identifier.issnl0003-455X-

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