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- Publisher Website: 10.1084/jem.151.6.1477
- Scopus: eid_2-s2.0-0018870460
- PMID: 6929880
- WOS: WOS:A1980JV38500013
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Article: Anemia- and polycythemia-inducing isolates of Friend spleen focus-forming virus. Biological and molecular evidence for two distinct viral genomes
Title | Anemia- and polycythemia-inducing isolates of Friend spleen focus-forming virus. Biological and molecular evidence for two distinct viral genomes |
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Authors | |
Issue Date | 1980 |
Citation | Journal of Experimental Medicine, 1980, v. 151, n. 6, p. 1477-1492 How to Cite? |
Abstract | Two distinct clones of Friend spleen focus-forming virus (SFFV), differing in their erythroleukemic potential, are described. These isolates have been cloned free of their associated helper viruses and shown to be replication-defective. Both SFFV isolates have been rescued from rat fibroblast nonproducer cell clones with cloned replication-competent viruses, F-MuLV(A) and F-MuLV(P), obtained from the anemia- or polycythemia-inducing isolates of Friend virus complex, respectively. These rescued viruses induce a rapid proliferative disease associated with the appearance of macroscopic spleen foci and splenomegaly. In addition, each is subject to regulation by the W, Steel (Sl), and Fv-2 host gene loci. These two isolates of SFFV can, however, be distinguished by both biological and molecular criteria. Friend SFFV(P) induces a rapid polycythemia associated with the appearance of large numbers of erythropoietin (EPO)-independent erythroid colony-forming cells in the marrow and spleen. In contrast, SFFV(A) induces a rapid anemia associated with a progressive decrease in the number of EPO-dependent erythroid colony-forming cells in marrow, and a rapid increase in the number of EPO-dependent erythroid colony-forming cells in spleen. Furthermore, the nature of the disease induced by the two isolates of SFFV is independent of the Friend helper virus: SFFV(P), rescued from a nonproducer cell clone with either F-MuLV(A) or F-MuLV(P), induced an anemic transformation. The two Friend SFFV isolates can also be discriminated on the basis of translational products encoded by their gag and env genes: SFFV(P) encodes the amino-terminal gag-gene protein p15, whereas SFFV(A) encodes nonidentical 55,000-mol wt env gene-related proteins p15, p12 and p30. In addition, the SFFV isolates encode nonidentical 55,000-mol wt env gene-related proteins that can be distinguished by analysis of their methionine-containing tryptic peptides. |
Persistent Identifier | http://hdl.handle.net/10722/292255 |
ISSN | 2023 Impact Factor: 12.6 2023 SCImago Journal Rankings: 6.838 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | MacDonald, M. E. | - |
dc.contributor.author | Reynolds, F. H. | - |
dc.contributor.author | van De Ven, W. J.M. | - |
dc.contributor.author | Stephenson, J. R. | - |
dc.contributor.author | Mak, T. W. | - |
dc.contributor.author | Bernstein, A. | - |
dc.date.accessioned | 2020-11-17T14:56:05Z | - |
dc.date.available | 2020-11-17T14:56:05Z | - |
dc.date.issued | 1980 | - |
dc.identifier.citation | Journal of Experimental Medicine, 1980, v. 151, n. 6, p. 1477-1492 | - |
dc.identifier.issn | 0022-1007 | - |
dc.identifier.uri | http://hdl.handle.net/10722/292255 | - |
dc.description.abstract | Two distinct clones of Friend spleen focus-forming virus (SFFV), differing in their erythroleukemic potential, are described. These isolates have been cloned free of their associated helper viruses and shown to be replication-defective. Both SFFV isolates have been rescued from rat fibroblast nonproducer cell clones with cloned replication-competent viruses, F-MuLV(A) and F-MuLV(P), obtained from the anemia- or polycythemia-inducing isolates of Friend virus complex, respectively. These rescued viruses induce a rapid proliferative disease associated with the appearance of macroscopic spleen foci and splenomegaly. In addition, each is subject to regulation by the W, Steel (Sl), and Fv-2 host gene loci. These two isolates of SFFV can, however, be distinguished by both biological and molecular criteria. Friend SFFV(P) induces a rapid polycythemia associated with the appearance of large numbers of erythropoietin (EPO)-independent erythroid colony-forming cells in the marrow and spleen. In contrast, SFFV(A) induces a rapid anemia associated with a progressive decrease in the number of EPO-dependent erythroid colony-forming cells in marrow, and a rapid increase in the number of EPO-dependent erythroid colony-forming cells in spleen. Furthermore, the nature of the disease induced by the two isolates of SFFV is independent of the Friend helper virus: SFFV(P), rescued from a nonproducer cell clone with either F-MuLV(A) or F-MuLV(P), induced an anemic transformation. The two Friend SFFV isolates can also be discriminated on the basis of translational products encoded by their gag and env genes: SFFV(P) encodes the amino-terminal gag-gene protein p15, whereas SFFV(A) encodes nonidentical 55,000-mol wt env gene-related proteins p15, p12 and p30. In addition, the SFFV isolates encode nonidentical 55,000-mol wt env gene-related proteins that can be distinguished by analysis of their methionine-containing tryptic peptides. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Experimental Medicine | - |
dc.title | Anemia- and polycythemia-inducing isolates of Friend spleen focus-forming virus. Biological and molecular evidence for two distinct viral genomes | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1084/jem.151.6.1477 | - |
dc.identifier.pmid | 6929880 | - |
dc.identifier.pmcid | PMC2185875 | - |
dc.identifier.scopus | eid_2-s2.0-0018870460 | - |
dc.identifier.volume | 151 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1477 | - |
dc.identifier.epage | 1492 | - |
dc.identifier.isi | WOS:A1980JV38500013 | - |
dc.identifier.issnl | 0022-1007 | - |