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Article: CD45RA and CD45RBhigh expression induced by thymic selection events

TitleCD45RA and CD45RB<sup>high</sup> expression induced by thymic selection events
Authors
Issue Date1992
Citation
Journal of Experimental Medicine, 1992, v. 176, n. 6, p. 1657-1663 How to Cite?
AbstractCD45 is a protein tyrosine phosphatase involved in T and B cell signaling. While peripheral T cells switch CD45 isoforms upon activation, events leading to exon switching during T cell development in the thymus have not been determined. The expression of high molecular weight isoforms of CD45 was examined on thymocytes from nontransgenic and T cell receptor (TCR) transgenic mice. All thymocytes from nontransgenic mice were CD45RB+ as assessed by staining with MB23G2, an anti-CD45RB-specific monoclonal antibody. Interestingly, there was a small population (1-3%) of thymocytes that displayed a higher intensity of staining with MB23G2, CD45RBhigh. CD45RBhigh thymocytes were found in all subsets defined by CD4 and CD8 expression and were also present within the TCR-α/βhigh population. To analyze whether or not CD45 expression correlated with thymic selection events, expression of CD45tLB high and a second isoform, CD45RA, was examined on thymocytes from H-Y and 2C TCR transgenic mice and found to correlate with positive and negative selection events but did not occur in nonselecting backgrounds. CD45RA and CD45RBhigh upregulation was also not observed in transgenic mice backcrossed into CD8-deficient mice, a scenario in which there is no positive selection of transgene-expressing thymocytes. These data suggest that modulation of CD45 isoform expression may be involved in thymic selection events. © 1992, Rockefeller University Press., All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/292392
ISSN
2023 Impact Factor: 12.6
2023 SCImago Journal Rankings: 6.838
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWallace, Valerie A.-
dc.contributor.authorFung-Leung, Wai Ping-
dc.contributor.authorTimms, Emma-
dc.contributor.authorGray, Dawn-
dc.contributor.authorKishihara, Kenji-
dc.contributor.authorLoh, Dennis Y.-
dc.contributor.authorPenninger, Josef-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:56:23Z-
dc.date.available2020-11-17T14:56:23Z-
dc.date.issued1992-
dc.identifier.citationJournal of Experimental Medicine, 1992, v. 176, n. 6, p. 1657-1663-
dc.identifier.issn0022-1007-
dc.identifier.urihttp://hdl.handle.net/10722/292392-
dc.description.abstractCD45 is a protein tyrosine phosphatase involved in T and B cell signaling. While peripheral T cells switch CD45 isoforms upon activation, events leading to exon switching during T cell development in the thymus have not been determined. The expression of high molecular weight isoforms of CD45 was examined on thymocytes from nontransgenic and T cell receptor (TCR) transgenic mice. All thymocytes from nontransgenic mice were CD45RB+ as assessed by staining with MB23G2, an anti-CD45RB-specific monoclonal antibody. Interestingly, there was a small population (1-3%) of thymocytes that displayed a higher intensity of staining with MB23G2, CD45RBhigh. CD45RBhigh thymocytes were found in all subsets defined by CD4 and CD8 expression and were also present within the TCR-α/βhigh population. To analyze whether or not CD45 expression correlated with thymic selection events, expression of CD45tLB high and a second isoform, CD45RA, was examined on thymocytes from H-Y and 2C TCR transgenic mice and found to correlate with positive and negative selection events but did not occur in nonselecting backgrounds. CD45RA and CD45RBhigh upregulation was also not observed in transgenic mice backcrossed into CD8-deficient mice, a scenario in which there is no positive selection of transgene-expressing thymocytes. These data suggest that modulation of CD45 isoform expression may be involved in thymic selection events. © 1992, Rockefeller University Press., All rights reserved.-
dc.languageeng-
dc.relation.ispartofJournal of Experimental Medicine-
dc.titleCD45RA and CD45RB<sup>high</sup> expression induced by thymic selection events-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1084/jem.176.6.1657-
dc.identifier.pmid1460424-
dc.identifier.pmcidPMC2119470-
dc.identifier.scopuseid_2-s2.0-0026441382-
dc.identifier.volume176-
dc.identifier.issue6-
dc.identifier.spage1657-
dc.identifier.epage1663-
dc.identifier.eissn1540-9538-
dc.identifier.isiWOS:A1992KA48100021-
dc.identifier.issnl0022-1007-

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