File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Class II major histocompatibility complex‐restricted T cell function in CD4‐deficient mice

TitleClass II major histocompatibility complex‐restricted T cell function in CD4‐deficient mice
Authors
KeywordsCD4‐deficient mice
Helper T cells
Immunoglobulin class‐switching
Issue Date1994
Citation
European Journal of Immunology, 1994, v. 24, n. 9, p. 2213-2218 How to Cite?
AbstractPreviously, we and others have demonstrated that CD4‐deficient mice have a normal number of T cells and B cells with a significant population of CD4‐8‐TcRαβ+ T cells. Surprisingly, however, these mice lacking CD4 show in vivo immunoglobulin isotype class switching from IgM to IgG in response to sheep erythrocytes and vesicular stomatitis virus. In this study we have depleted various subpopulations of T cells in vivo and shown that the population of CD4‐8‐TcRαβ+ T cells is responsible for providing “help” in the antibody response of CD4‐deficient mice to vesicular stomatitis virus infection. We have used antigen‐specific proliferation assays and blocking studies with class I and II major histocompatibility complex (MHC)‐specific purified antibodies to show that these cells are class II MHC‐restricted in responses against the T cell‐dependent antigen keyhole limpet hemocyanin (KLH). Finally, phenotypic analysis of the CD4‐ CD8‐thymocytes in CD4‐deficient mice shows that these cells have a more mature phenotype than the CD4‐8‐ thymocytes in wild type mice. These results indicate that CD4 is not absolutely necessary for positive selection or effector function of class II MHC‐restricted helper T cells. Copyright © 1994 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent Identifierhttp://hdl.handle.net/10722/292437
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.627
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRahemtulla, Amin-
dc.contributor.authorKündig, Thomas M.-
dc.contributor.authorNarendran, Arumugabadiv-
dc.contributor.authorBachmann, Martin F.-
dc.contributor.authorJulius, Michael-
dc.contributor.authorPaige, Christopher J.-
dc.contributor.authorOhashi, Pamela S.-
dc.contributor.authorZinkernagel, Rolf M.-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:56:29Z-
dc.date.available2020-11-17T14:56:29Z-
dc.date.issued1994-
dc.identifier.citationEuropean Journal of Immunology, 1994, v. 24, n. 9, p. 2213-2218-
dc.identifier.issn0014-2980-
dc.identifier.urihttp://hdl.handle.net/10722/292437-
dc.description.abstractPreviously, we and others have demonstrated that CD4‐deficient mice have a normal number of T cells and B cells with a significant population of CD4‐8‐TcRαβ+ T cells. Surprisingly, however, these mice lacking CD4 show in vivo immunoglobulin isotype class switching from IgM to IgG in response to sheep erythrocytes and vesicular stomatitis virus. In this study we have depleted various subpopulations of T cells in vivo and shown that the population of CD4‐8‐TcRαβ+ T cells is responsible for providing “help” in the antibody response of CD4‐deficient mice to vesicular stomatitis virus infection. We have used antigen‐specific proliferation assays and blocking studies with class I and II major histocompatibility complex (MHC)‐specific purified antibodies to show that these cells are class II MHC‐restricted in responses against the T cell‐dependent antigen keyhole limpet hemocyanin (KLH). Finally, phenotypic analysis of the CD4‐ CD8‐thymocytes in CD4‐deficient mice shows that these cells have a more mature phenotype than the CD4‐8‐ thymocytes in wild type mice. These results indicate that CD4 is not absolutely necessary for positive selection or effector function of class II MHC‐restricted helper T cells. Copyright © 1994 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.languageeng-
dc.relation.ispartofEuropean Journal of Immunology-
dc.subjectCD4‐deficient mice-
dc.subjectHelper T cells-
dc.subjectImmunoglobulin class‐switching-
dc.titleClass II major histocompatibility complex‐restricted T cell function in CD4‐deficient mice-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/eji.1830240942-
dc.identifier.pmid7916297-
dc.identifier.scopuseid_2-s2.0-0028136223-
dc.identifier.volume24-
dc.identifier.issue9-
dc.identifier.spage2213-
dc.identifier.epage2218-
dc.identifier.eissn1521-4141-
dc.identifier.isiWOS:A1994PJ30000041-
dc.identifier.issnl0014-2980-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats