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Article: T lymphocyte development in p56lck deficient mice: allelic exclusion of the TcR β locus is incomplete but thymocyte development is not restored by TcR β or TcR αβ transgenes

TitleT lymphocyte development in p<sup>56</sup><sup>lck</sup> deficient mice: allelic exclusion of the TcR β locus is incomplete but thymocyte development is not restored by TcR β or TcR αβ transgenes
Authors
KeywordsT lymphocyte development
T cell receptor β chain
p56 lck
Issue Date1995
Citation
European Journal of Immunology, 1995, v. 25, n. 5, p. 1312-1318 How to Cite?
AbstractThe protein tyrosine kinase, p56lck, is involved in signal transduction in mature T cells and in the molecular events controlling early thymocyte differentiation. Thymuses of mice deficient for p56lck expression (p56lck‐/‐) consist of immature CD4‐CD8‐ double‐negative (DN) and CD4+CD8+ double‐positive (DP) thymocytes and are severely reduced in total cell number. In this report we have studied DN thymocytes from p56lck‐/‐ mice and found an increase in the proportion of the CD44−CD25+ subset, suggesting that transit through this stage, which is known to require T cell receptor (TcR) β expression, may be delayed in the absence of p56lck expression. In addition, the expression of a transgenic TcR β chain or TcR αβ pair did not restore thymic development in p56lck‐/‐ mice. However, in contrast to mice expressing a dominant negative isoform of p56lck in which DP thymocytes do not develop, DP thymocytes still develop in nontransgenic and TcR transgenic p56lck‐/‐ mice. These results demonstrate that expansion of the DP subset is impaired in p56lck‐/‐ mice. In contrast, allelic exclusion is not severely compromised. Although there was an increase in the number of peripheral T cells expressing more than one Vβ chain in TcR transgenic p56lck‐/‐ mice, we found that inhibition of endogenous TcR β gene rearrangement was almost complete in thymocytes of Vβ transgenic p56lck‐/‐ mice and we could not detect any peripheral T cells that expressed more than one Vβ chain in non‐transgenic p56lck‐/‐ mice. Copyright © 1995 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Persistent Identifierhttp://hdl.handle.net/10722/292463
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.627
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWallace, Valerie A.-
dc.contributor.authorKawai, Kazuhiro-
dc.contributor.authorLevelt, Christiaan N.-
dc.contributor.authorKishihara, Kenji-
dc.contributor.authorMolina, Thierry-
dc.contributor.authorTimms, Emma-
dc.contributor.authorPircher, Hanspeter-
dc.contributor.authorPenninger, Josef-
dc.contributor.authorOhashi, Pamela S.-
dc.contributor.authorEichmann, Klaus-
dc.contributor.authorMak, Tak W.-
dc.date.accessioned2020-11-17T14:56:32Z-
dc.date.available2020-11-17T14:56:32Z-
dc.date.issued1995-
dc.identifier.citationEuropean Journal of Immunology, 1995, v. 25, n. 5, p. 1312-1318-
dc.identifier.issn0014-2980-
dc.identifier.urihttp://hdl.handle.net/10722/292463-
dc.description.abstractThe protein tyrosine kinase, p56lck, is involved in signal transduction in mature T cells and in the molecular events controlling early thymocyte differentiation. Thymuses of mice deficient for p56lck expression (p56lck‐/‐) consist of immature CD4‐CD8‐ double‐negative (DN) and CD4+CD8+ double‐positive (DP) thymocytes and are severely reduced in total cell number. In this report we have studied DN thymocytes from p56lck‐/‐ mice and found an increase in the proportion of the CD44−CD25+ subset, suggesting that transit through this stage, which is known to require T cell receptor (TcR) β expression, may be delayed in the absence of p56lck expression. In addition, the expression of a transgenic TcR β chain or TcR αβ pair did not restore thymic development in p56lck‐/‐ mice. However, in contrast to mice expressing a dominant negative isoform of p56lck in which DP thymocytes do not develop, DP thymocytes still develop in nontransgenic and TcR transgenic p56lck‐/‐ mice. These results demonstrate that expansion of the DP subset is impaired in p56lck‐/‐ mice. In contrast, allelic exclusion is not severely compromised. Although there was an increase in the number of peripheral T cells expressing more than one Vβ chain in TcR transgenic p56lck‐/‐ mice, we found that inhibition of endogenous TcR β gene rearrangement was almost complete in thymocytes of Vβ transgenic p56lck‐/‐ mice and we could not detect any peripheral T cells that expressed more than one Vβ chain in non‐transgenic p56lck‐/‐ mice. Copyright © 1995 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.languageeng-
dc.relation.ispartofEuropean Journal of Immunology-
dc.subjectT lymphocyte development-
dc.subjectT cell receptor β chain-
dc.subjectp56 lck-
dc.titleT lymphocyte development in p<sup>56</sup><sup>lck</sup> deficient mice: allelic exclusion of the TcR β locus is incomplete but thymocyte development is not restored by TcR β or TcR αβ transgenes-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/eji.1830250527-
dc.identifier.pmid7774634-
dc.identifier.scopuseid_2-s2.0-0029004406-
dc.identifier.volume25-
dc.identifier.issue5-
dc.identifier.spage1312-
dc.identifier.epage1318-
dc.identifier.eissn1521-4141-
dc.identifier.isiWOS:A1995RC85900026-
dc.identifier.issnl0014-2980-

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