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Article: Design, synthesis and evaluation of DNA nano-cubes as a core material protected by the alginate coating for oral administration of anti-diabetic drug

TitleDesign, synthesis and evaluation of DNA nano-cubes as a core material protected by the alginate coating for oral administration of anti-diabetic drug
Authors
KeywordsVildagliptin
Type 2 diabetics
3D DNA nanocube
Db–Db/mice
Nanospheres
Issue Date2019
Citation
Journal of Food and Drug Analysis, 2019, v. 27, n. 3, p. 805-814 How to Cite?
AbstractPoor control towards glycemic levels among diabetic patients may lead to severe micro/macro-vascular and neuropathic complexities. Proper functioning of alpha-beta cells of pancreases is required to attain long term glycemic control among type 2 diabetics. The recent developments to manage diabetes are focused on controlling the insulin-glucagon secretions from the pancreases. DPP-4 inhibitors class of drugs after elevating GLP-1/GIP (incretins) levels in the blood, not only raise the insulin levels but also suppress the glucagon level. Vildagliptin (VI) is a potent DPP-4 inhibitor with least adverse events compared to other DPP-4 inhibitors. We encapsulated VI into 3D nanocube that gets bind to the DNA due to secondary amine in its chemical structure. DNA-nanocube being negatively charged was incubated with the PLL to attain positive surface. Ultimately VI loaded nanocubes were coated with the negatively charged Na-alginate via electrostatic attraction method to get stable spherical nanospheres for oral delivery of VI. Nanospheres were evaluated physically through native PAGE analysis, DSC, TGA, dissolution testing, XRD and FTIR. We attained uniformed and spherical nanospheres with stable topology, nanoscale size precision (40–150 nm in diameter), Entrapment efficiency (up to 90%), prolonged drug release (13 ± 4 h) at basic pH, and superior oral antidiabetic effects with improved GLP1 and glycemic levels. The formulated nanospheres attained size uniformity and better therapeutic outcomes in terms of reduced adverse events and better control of glycemic levels than previously reported methods with decreased dosage frequency tested in Db/Db mice.
Persistent Identifierhttp://hdl.handle.net/10722/293114
ISSN
2023 Impact Factor: 2.6
2023 SCImago Journal Rankings: 0.609
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBaig, Mirza Muhammad Faran Ashraf-
dc.contributor.authorAbbas, Muhammad-
dc.contributor.authorNaveed, Muhammad-
dc.contributor.authorKassim, Said Abasse-
dc.contributor.authorKhan, Ghulam Jilany-
dc.contributor.authorSohail, Muhammad-
dc.contributor.authorUllah, Sana-
dc.contributor.authorHasnat, Muhammad-
dc.contributor.authorShah, Komal-
dc.contributor.authorAnsari, Muhammad Tayyab-
dc.date.accessioned2020-11-19T09:02:00Z-
dc.date.available2020-11-19T09:02:00Z-
dc.date.issued2019-
dc.identifier.citationJournal of Food and Drug Analysis, 2019, v. 27, n. 3, p. 805-814-
dc.identifier.issn1021-9498-
dc.identifier.urihttp://hdl.handle.net/10722/293114-
dc.description.abstractPoor control towards glycemic levels among diabetic patients may lead to severe micro/macro-vascular and neuropathic complexities. Proper functioning of alpha-beta cells of pancreases is required to attain long term glycemic control among type 2 diabetics. The recent developments to manage diabetes are focused on controlling the insulin-glucagon secretions from the pancreases. DPP-4 inhibitors class of drugs after elevating GLP-1/GIP (incretins) levels in the blood, not only raise the insulin levels but also suppress the glucagon level. Vildagliptin (VI) is a potent DPP-4 inhibitor with least adverse events compared to other DPP-4 inhibitors. We encapsulated VI into 3D nanocube that gets bind to the DNA due to secondary amine in its chemical structure. DNA-nanocube being negatively charged was incubated with the PLL to attain positive surface. Ultimately VI loaded nanocubes were coated with the negatively charged Na-alginate via electrostatic attraction method to get stable spherical nanospheres for oral delivery of VI. Nanospheres were evaluated physically through native PAGE analysis, DSC, TGA, dissolution testing, XRD and FTIR. We attained uniformed and spherical nanospheres with stable topology, nanoscale size precision (40–150 nm in diameter), Entrapment efficiency (up to 90%), prolonged drug release (13 ± 4 h) at basic pH, and superior oral antidiabetic effects with improved GLP1 and glycemic levels. The formulated nanospheres attained size uniformity and better therapeutic outcomes in terms of reduced adverse events and better control of glycemic levels than previously reported methods with decreased dosage frequency tested in Db/Db mice.-
dc.languageeng-
dc.relation.ispartofJournal of Food and Drug Analysis-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectVildagliptin-
dc.subjectType 2 diabetics-
dc.subject3D DNA nanocube-
dc.subjectDb–Db/mice-
dc.subjectNanospheres-
dc.titleDesign, synthesis and evaluation of DNA nano-cubes as a core material protected by the alginate coating for oral administration of anti-diabetic drug-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.jfda.2019.03.004-
dc.identifier.pmid31324296-
dc.identifier.scopuseid_2-s2.0-85064595320-
dc.identifier.volume27-
dc.identifier.issue3-
dc.identifier.spage805-
dc.identifier.epage814-
dc.identifier.isiWOS:000475780800019-
dc.identifier.issnl1021-9498-

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