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- Publisher Website: 10.1016/j.yexcr.2019.111718
- Scopus: eid_2-s2.0-85077643232
- PMID: 31874176
- WOS: WOS:000515211600012
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Article: Regulation of human trophoblast surrogate Jeg-3 spheroids implantation potential by Wnt/β-catenin pathway and lin28a/let-7a axis
Title | Regulation of human trophoblast surrogate Jeg-3 spheroids implantation potential by Wnt/β-catenin pathway and lin28a/let-7a axis |
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Authors | |
Keywords | Wnt/β-catenin signalling Lin28a protein mir-let-7a Jeg-3 spheroids |
Issue Date | 2020 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr |
Citation | Experimental Cell Research, 2020, v. 388 n. 1, p. article no. 111718 How to Cite? |
Abstract | Successful implantation happens only when the development of a competent blastocyst synchronized with the differentiation of a receptive uterus. The exact mechanism affecting embryo implantation competency is still unclear. Previous data from our laboratory showed that several members of the let-7 family were up-regulated in the implanting dormant blastocysts and prohibited embryo activation through down-regulation integrin-β3. However, how the mir-let-7 family is regulated is still a question. In this study, the in vitro co-culture model was applied to imitate implantation. Human embryo surrogate Jeg-3 spheroids and endometrium epithelial cells Ishikawa were used. The following views were demonstrated. Firstly,Wnt/β-catenin signaling is essential for Jeg-3 spheroids implantation. Secondly, mir-let-7a is repressed by Wnt signaling, and low let-7a is beneficial for spheroids attachment and outgrowth. Third, in contrast with let-7a, lin28a is up-regulated by Wnt and promotes attachment and outgrowth. Lastly, the function of Wnt in embryo surrogate spheroids in implantation is mediated through lin28a/let-7a axis. In summary, our findings suggest Wnt/β-catenin signaling strength human embryo surrogate spheroids implanting potential through regulation lin28a/let-7a axis. |
Persistent Identifier | http://hdl.handle.net/10722/293449 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.947 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LI, Q | - |
dc.contributor.author | Chiu, PCN | - |
dc.contributor.author | Yeung, WSB | - |
dc.contributor.author | Liu, W | - |
dc.date.accessioned | 2020-11-23T08:16:55Z | - |
dc.date.available | 2020-11-23T08:16:55Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Experimental Cell Research, 2020, v. 388 n. 1, p. article no. 111718 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.uri | http://hdl.handle.net/10722/293449 | - |
dc.description.abstract | Successful implantation happens only when the development of a competent blastocyst synchronized with the differentiation of a receptive uterus. The exact mechanism affecting embryo implantation competency is still unclear. Previous data from our laboratory showed that several members of the let-7 family were up-regulated in the implanting dormant blastocysts and prohibited embryo activation through down-regulation integrin-β3. However, how the mir-let-7 family is regulated is still a question. In this study, the in vitro co-culture model was applied to imitate implantation. Human embryo surrogate Jeg-3 spheroids and endometrium epithelial cells Ishikawa were used. The following views were demonstrated. Firstly,Wnt/β-catenin signaling is essential for Jeg-3 spheroids implantation. Secondly, mir-let-7a is repressed by Wnt signaling, and low let-7a is beneficial for spheroids attachment and outgrowth. Third, in contrast with let-7a, lin28a is up-regulated by Wnt and promotes attachment and outgrowth. Lastly, the function of Wnt in embryo surrogate spheroids in implantation is mediated through lin28a/let-7a axis. In summary, our findings suggest Wnt/β-catenin signaling strength human embryo surrogate spheroids implanting potential through regulation lin28a/let-7a axis. | - |
dc.language | eng | - |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr | - |
dc.relation.ispartof | Experimental Cell Research | - |
dc.subject | Wnt/β-catenin signalling | - |
dc.subject | Lin28a protein | - |
dc.subject | mir-let-7a | - |
dc.subject | Jeg-3 spheroids | - |
dc.title | Regulation of human trophoblast surrogate Jeg-3 spheroids implantation potential by Wnt/β-catenin pathway and lin28a/let-7a axis | - |
dc.type | Article | - |
dc.identifier.email | Chiu, PCN: pchiucn@hku.hk | - |
dc.identifier.email | Yeung, WSB: wsbyeung@hku.hk | - |
dc.identifier.email | Liu, W: liuwm@hkucc.hku.hk | - |
dc.identifier.authority | Chiu, PCN=rp00424 | - |
dc.identifier.authority | Yeung, WSB=rp00331 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.yexcr.2019.111718 | - |
dc.identifier.pmid | 31874176 | - |
dc.identifier.scopus | eid_2-s2.0-85077643232 | - |
dc.identifier.hkuros | 319423 | - |
dc.identifier.volume | 388 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 111718 | - |
dc.identifier.epage | article no. 111718 | - |
dc.identifier.isi | WOS:000515211600012 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0014-4827 | - |