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Article: Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China

TitleMetagenomics of urban sewage identifies an extensively shared antibiotic resistome in China
Authors
KeywordsAntibiotic resistance
Environment
Pollution
Evolution
Human gut microbiome
Issue Date2017
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.microbiomejournal.com/
Citation
Microbiome, 2017, v. 5 n. 1, p. article no. 84 How to Cite?
AbstractBackground: Antibiotic-resistant pathogens are challenging treatment of infections worldwide. Urban sewage is potentially a major conduit for dissemination of antibiotic resistance genes into various environmental compartments. However, the diversity and abundance of such genes in wastewater are not well known. Methods: Here, seasonal and geographical distributions of antibiotic resistance genes and their host bacterial communities from Chinese urban sewage were characterized, using metagenomic analyses and 16S rRNA gene-based Illumina sequencing, respectively. Results: In total, 381 different resistance genes were detected, and these genes were extensively shared across China, with no geographical clustering. Seasonal variation in abundance of resistance genes was observed, with average concentrations of 3.27 × 1011 and 1.79 × 1012 copies/L in summer and winter, respectively. Bacterial communities did not exhibit geographical clusters, but did show a significant distance-decay relationship (P < 0.01). The core, shared resistome accounted for 57.7% of the total resistance genes, and was significantly associated with the core microbial community (P < 0.01). The core human gut microbiota was also strongly associated with the shared resistome, demonstrating the potential contribution of human gut microbiota to the dissemination of resistance elements via sewage disposal. Conclusions: This study provides a baseline for investigating environmental dissemination of resistance elements and raises the possibility of using the abundance of resistance genes in sewage as a tool for antibiotic stewardship.
Persistent Identifierhttp://hdl.handle.net/10722/293569
ISSN
2023 Impact Factor: 13.8
2023 SCImago Journal Rankings: 3.802
PubMed Central ID
ISI Accession Number ID
Errata

 

DC FieldValueLanguage
dc.contributor.authorSu, JQ-
dc.contributor.authorAn, XL-
dc.contributor.authorLi, B-
dc.contributor.authorChen, Q-
dc.contributor.authorGillings, MR-
dc.contributor.authorChen, H-
dc.contributor.authorZhang, T-
dc.contributor.authorZhu, YG-
dc.date.accessioned2020-11-23T08:18:41Z-
dc.date.available2020-11-23T08:18:41Z-
dc.date.issued2017-
dc.identifier.citationMicrobiome, 2017, v. 5 n. 1, p. article no. 84-
dc.identifier.issn2049-2618-
dc.identifier.urihttp://hdl.handle.net/10722/293569-
dc.description.abstractBackground: Antibiotic-resistant pathogens are challenging treatment of infections worldwide. Urban sewage is potentially a major conduit for dissemination of antibiotic resistance genes into various environmental compartments. However, the diversity and abundance of such genes in wastewater are not well known. Methods: Here, seasonal and geographical distributions of antibiotic resistance genes and their host bacterial communities from Chinese urban sewage were characterized, using metagenomic analyses and 16S rRNA gene-based Illumina sequencing, respectively. Results: In total, 381 different resistance genes were detected, and these genes were extensively shared across China, with no geographical clustering. Seasonal variation in abundance of resistance genes was observed, with average concentrations of 3.27 × 1011 and 1.79 × 1012 copies/L in summer and winter, respectively. Bacterial communities did not exhibit geographical clusters, but did show a significant distance-decay relationship (P < 0.01). The core, shared resistome accounted for 57.7% of the total resistance genes, and was significantly associated with the core microbial community (P < 0.01). The core human gut microbiota was also strongly associated with the shared resistome, demonstrating the potential contribution of human gut microbiota to the dissemination of resistance elements via sewage disposal. Conclusions: This study provides a baseline for investigating environmental dissemination of resistance elements and raises the possibility of using the abundance of resistance genes in sewage as a tool for antibiotic stewardship.-
dc.languageeng-
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.microbiomejournal.com/-
dc.relation.ispartofMicrobiome-
dc.rightsMicrobiome. Copyright © BioMed Central Ltd.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectAntibiotic resistance-
dc.subjectEnvironment-
dc.subjectPollution-
dc.subjectEvolution-
dc.subjectHuman gut microbiome-
dc.titleMetagenomics of urban sewage identifies an extensively shared antibiotic resistome in China-
dc.typeArticle-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s40168-017-0298-y-
dc.identifier.pmid28724443-
dc.identifier.pmcidPMC5517792-
dc.identifier.scopuseid_2-s2.0-85033488446-
dc.identifier.hkuros319386-
dc.identifier.volume5-
dc.identifier.issue1-
dc.identifier.spagearticle no. 84-
dc.identifier.epagearticle no. 84-
dc.identifier.isiWOS:000405898200003-
dc.publisher.placeUnited Kingdom-
dc.relation.erratumdoi:10.1186/s40168-018-0504-6-
dc.identifier.issnl2049-2618-

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