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Article: Shift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment

TitleShift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment
Authors
KeywordsAntibiotic resistance genes
nanosilver
metagenomic analysis
biological wastewater treatment
sequencing batch reactors
Issue Date2016
PublisherOxford University Press. The Journal's web site is located at http://femsec.oxfordjournals.org/
Citation
FEMS Microbiology Ecology, 2016, v. 92 n. 3, p. article no. fiw022 How to Cite?
AbstractThis study investigated the response of antibiotic resistance genes (ARGs) to nanosilver (Ag) in lab-scale nitrifying sequencing batch reactors (SBRs), compared to Ag+-dosed and undosed controls. Quantitative polymerase chain reaction (q-PCR) targeting sul1, tet(O), ermB and the class I integron gene intI1 and corresponding RNA expression did not indicate measureable effects of nanoAg or Ag+ on abundance or expression of these genes. However, high-throughput sequencing based metagenomic analysis provided a much broader profile of gene responses and revealed a greater abundance of aminoglycoside resistance genes (mainly strA) in reactors dosed with nanoAg. In contrast, bacitracin and macrolide-lincosamide-streptogramin (MLS) resistance genes were more abundant in the SBRs dosed with Ag+. The distinct ARG profiles associated with nanoAg and Ag+ were correlated with the taxonomic composition of the microbial communities. This study indicates that nanoAg may interact with bacteria differently from Ag+ during biological wastewater treatment. Therefore, it cannot necessarily be assumed that nanosilver behaves identically as Ag+ when conducting a risk assessment for release into the environment.
DescriptionLink to Free access
Persistent Identifierhttp://hdl.handle.net/10722/293585
ISSN
2023 Impact Factor: 3.5
2023 SCImago Journal Rankings: 1.069
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMa, Y-
dc.contributor.authorMetch, JW-
dc.contributor.authorYang, Y-
dc.contributor.authorPruden, A-
dc.contributor.authorZhang, T-
dc.date.accessioned2020-11-23T08:18:54Z-
dc.date.available2020-11-23T08:18:54Z-
dc.date.issued2016-
dc.identifier.citationFEMS Microbiology Ecology, 2016, v. 92 n. 3, p. article no. fiw022-
dc.identifier.issn0168-6496-
dc.identifier.urihttp://hdl.handle.net/10722/293585-
dc.descriptionLink to Free access-
dc.description.abstractThis study investigated the response of antibiotic resistance genes (ARGs) to nanosilver (Ag) in lab-scale nitrifying sequencing batch reactors (SBRs), compared to Ag+-dosed and undosed controls. Quantitative polymerase chain reaction (q-PCR) targeting sul1, tet(O), ermB and the class I integron gene intI1 and corresponding RNA expression did not indicate measureable effects of nanoAg or Ag+ on abundance or expression of these genes. However, high-throughput sequencing based metagenomic analysis provided a much broader profile of gene responses and revealed a greater abundance of aminoglycoside resistance genes (mainly strA) in reactors dosed with nanoAg. In contrast, bacitracin and macrolide-lincosamide-streptogramin (MLS) resistance genes were more abundant in the SBRs dosed with Ag+. The distinct ARG profiles associated with nanoAg and Ag+ were correlated with the taxonomic composition of the microbial communities. This study indicates that nanoAg may interact with bacteria differently from Ag+ during biological wastewater treatment. Therefore, it cannot necessarily be assumed that nanosilver behaves identically as Ag+ when conducting a risk assessment for release into the environment.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://femsec.oxfordjournals.org/-
dc.relation.ispartofFEMS Microbiology Ecology-
dc.rightsPre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here].-
dc.subjectAntibiotic resistance genes-
dc.subjectnanosilver-
dc.subjectmetagenomic analysis-
dc.subjectbiological wastewater treatment-
dc.subjectsequencing batch reactors-
dc.titleShift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment-
dc.typeArticle-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/femsec/fiw022-
dc.identifier.pmid26850160-
dc.identifier.scopuseid_2-s2.0-84962159423-
dc.identifier.hkuros319457-
dc.identifier.volume92-
dc.identifier.issue3-
dc.identifier.spagearticle no. fiw022-
dc.identifier.epagearticle no. fiw022-
dc.identifier.isiWOS:000373295800016-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0168-6496-

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