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Article: CDR3 sequences in IgA nephropathy are shorter and exhibit reduced diversity

TitleCDR3 sequences in IgA nephropathy are shorter and exhibit reduced diversity
Authors
KeywordsCDR3 length
IgA
IgA nephropathy
IgG
IgM
Issue Date2020
PublisherFEBS Press and John Wiley & Sons Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%292211-5463
Citation
FEBS Open Bio, 2020, v. 10 n. 12, p. 2702-2711 How to Cite?
AbstractImmunoglobulin (Ig) A nephropathy (IgAN) is the most common glomerulonephritis, which is characterized by the deposition of IgA antibody in the glomerulus. Systematic dissection of immune composition may contribute to a better understanding of the alternations in the immune system in IgAN. To this end, here we applied immune repertoire sequencing technology for parallel analysis of the complementary determining region 3 (CDR3) of all B cell receptors, including all five antibody subtypes (IgA, IgG, IgM, IgE and IgD), in 13 patients with IgAN and 7 healthy individuals. A significant decrease in CDR3 length was observed in the IgAN group. In particular, the JH6 family was significantly increased in IgAN. Amino acid usage was also altered in IgAN. Shannon, Simpson, Gini and Diversity 50 indices also revealed significant differences in the diversity of IgG, IgM and IgA antibodies as compared with controls. The proportions of IgA and IgG were increased, whereas IgM was decreased in IgAN. Moreover, a greater number of CDR3 sequences was shared between patients with IgAN. These findings suggest that the BCR immune repertoire is dramatically altered in IgAN, as characterized by shortened CDR3 length, as well as decreased overall diversity of CDR3.
Persistent Identifierhttp://hdl.handle.net/10722/293874
ISSN
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.789
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, X-
dc.contributor.authorZeng, J-
dc.contributor.authorTong, Y-
dc.contributor.authorZhang, L-
dc.contributor.authorLu, X-
dc.contributor.authorZhu, S-
dc.contributor.authorLi, Z-
dc.date.accessioned2020-11-23T08:23:05Z-
dc.date.available2020-11-23T08:23:05Z-
dc.date.issued2020-
dc.identifier.citationFEBS Open Bio, 2020, v. 10 n. 12, p. 2702-2711-
dc.identifier.issn2211-5463-
dc.identifier.urihttp://hdl.handle.net/10722/293874-
dc.description.abstractImmunoglobulin (Ig) A nephropathy (IgAN) is the most common glomerulonephritis, which is characterized by the deposition of IgA antibody in the glomerulus. Systematic dissection of immune composition may contribute to a better understanding of the alternations in the immune system in IgAN. To this end, here we applied immune repertoire sequencing technology for parallel analysis of the complementary determining region 3 (CDR3) of all B cell receptors, including all five antibody subtypes (IgA, IgG, IgM, IgE and IgD), in 13 patients with IgAN and 7 healthy individuals. A significant decrease in CDR3 length was observed in the IgAN group. In particular, the JH6 family was significantly increased in IgAN. Amino acid usage was also altered in IgAN. Shannon, Simpson, Gini and Diversity 50 indices also revealed significant differences in the diversity of IgG, IgM and IgA antibodies as compared with controls. The proportions of IgA and IgG were increased, whereas IgM was decreased in IgAN. Moreover, a greater number of CDR3 sequences was shared between patients with IgAN. These findings suggest that the BCR immune repertoire is dramatically altered in IgAN, as characterized by shortened CDR3 length, as well as decreased overall diversity of CDR3.-
dc.languageeng-
dc.publisherFEBS Press and John Wiley & Sons Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%292211-5463-
dc.relation.ispartofFEBS Open Bio-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCDR3 length-
dc.subjectIgA-
dc.subjectIgA nephropathy-
dc.subjectIgG-
dc.subjectIgM-
dc.titleCDR3 sequences in IgA nephropathy are shorter and exhibit reduced diversity-
dc.typeArticle-
dc.identifier.emailTong, Y: tongyin9@hku.hk-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1002/2211-5463.13006-
dc.identifier.pmid33067920-
dc.identifier.pmcidPMC7714077-
dc.identifier.scopuseid_2-s2.0-85096719064-
dc.identifier.hkuros320206-
dc.identifier.volume10-
dc.identifier.issue12-
dc.identifier.spage2702-
dc.identifier.epage2711-
dc.identifier.isiWOS:000590602100001-
dc.publisher.placeUnited Kingdom-

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