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Article: Suppression of lncRNA MALAT1 by betulinic acid inhibits hepatocellular carcinoma progression by targeting IAPs via miR‐22‐3p

TitleSuppression of lncRNA MALAT1 by betulinic acid inhibits hepatocellular carcinoma progression by targeting IAPs via miR‐22‐3p
Authors
Keywordsapoptosis
autophagy
betulinic acid
cell death
hepatocellular carcinoma
Issue Date2020
PublisherWiley Open Access: Creative Commons Attribution License. The Journal's web site is located at http://www.clintransmed.com
Citation
Clinical and Translational Medicine, 2020, v. 10 n. 6, p. article no. e190 How to Cite?
AbstractBetulinic acid (BA) is a natural product extracted from a broad range of medicinal and edible herbal plants. Previous studies showed that BA induces cell death in tumors derived from multiple tissues; however, the underlying mechanism remains obscure. The present study aimed to study the effects of BA on autophagy and apoptosis of hepatocellular carcinoma (HCC). Human HCC cell lines and orthotopic HCC implanted mice were employed to examine the BA‐induced tumor suppression; RT2 long noncoding RNA (lncRNA) PCR array and database analysis were used to explore the possible mechanisms; validation of pathways was performed using siRNA and miRNA inhibitors. The results indicated that BA regulated autophagy and induced apoptosis in HCC. The degradation of inhibitor of apoptosis proteins (IAPs), the conversion of LC3‐I to LC3‐II, and p62 accumulation were enhanced by BA, thereby suggesting that the downregulation of IAPs and autophagic cell death are induced by BA. The addition of autophagy and lysosomal inhibitors indicated that BA induced autophagy‐independent apoptosis via degradation of IAPs. Moreover, RT2 lncRNA PCR array and database analysis suggested that BA downregulated the levels of lncRNA MALAT1, which is considered to be an oncogene. Further investigations demonstrated that lncRNA MALAT1 functioned as a ceRNA (competing endogenous RNA) to contribute to BA‐mediated degradation of IAPs by sponging miR‐22‐3p. Therefore, BA could be developed as a potential anticancer agent for HCC.
Persistent Identifierhttp://hdl.handle.net/10722/293946
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCHEN, F-
dc.contributor.authorZhong, Z-
dc.contributor.authorTan, HY-
dc.contributor.authorGUO, W-
dc.contributor.authorZHANG, C-
dc.contributor.authorCheng, CS-
dc.contributor.authorWang, N-
dc.contributor.authorRen, J-
dc.contributor.authorFeng, Y-
dc.date.accessioned2020-11-23T08:24:08Z-
dc.date.available2020-11-23T08:24:08Z-
dc.date.issued2020-
dc.identifier.citationClinical and Translational Medicine, 2020, v. 10 n. 6, p. article no. e190-
dc.identifier.urihttp://hdl.handle.net/10722/293946-
dc.description.abstractBetulinic acid (BA) is a natural product extracted from a broad range of medicinal and edible herbal plants. Previous studies showed that BA induces cell death in tumors derived from multiple tissues; however, the underlying mechanism remains obscure. The present study aimed to study the effects of BA on autophagy and apoptosis of hepatocellular carcinoma (HCC). Human HCC cell lines and orthotopic HCC implanted mice were employed to examine the BA‐induced tumor suppression; RT2 long noncoding RNA (lncRNA) PCR array and database analysis were used to explore the possible mechanisms; validation of pathways was performed using siRNA and miRNA inhibitors. The results indicated that BA regulated autophagy and induced apoptosis in HCC. The degradation of inhibitor of apoptosis proteins (IAPs), the conversion of LC3‐I to LC3‐II, and p62 accumulation were enhanced by BA, thereby suggesting that the downregulation of IAPs and autophagic cell death are induced by BA. The addition of autophagy and lysosomal inhibitors indicated that BA induced autophagy‐independent apoptosis via degradation of IAPs. Moreover, RT2 lncRNA PCR array and database analysis suggested that BA downregulated the levels of lncRNA MALAT1, which is considered to be an oncogene. Further investigations demonstrated that lncRNA MALAT1 functioned as a ceRNA (competing endogenous RNA) to contribute to BA‐mediated degradation of IAPs by sponging miR‐22‐3p. Therefore, BA could be developed as a potential anticancer agent for HCC.-
dc.languageeng-
dc.publisherWiley Open Access: Creative Commons Attribution License. The Journal's web site is located at http://www.clintransmed.com-
dc.relation.ispartofClinical and Translational Medicine-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectapoptosis-
dc.subjectautophagy-
dc.subjectbetulinic acid-
dc.subjectcell death-
dc.subjecthepatocellular carcinoma-
dc.titleSuppression of lncRNA MALAT1 by betulinic acid inhibits hepatocellular carcinoma progression by targeting IAPs via miR‐22‐3p-
dc.typeArticle-
dc.identifier.emailZhong, Z: zfzhong@hku.hk-
dc.identifier.emailTan, HY: hyhtan@hku.hk-
dc.identifier.emailCheng, CS: nc95760@hku.hk-
dc.identifier.emailWang, N: ckwang@hku.hk-
dc.identifier.emailFeng, Y: yfeng@hku.hk-
dc.identifier.authorityWang, N=rp02075-
dc.identifier.authorityFeng, Y=rp00466-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1002/ctm2.190-
dc.identifier.pmid33135336-
dc.identifier.pmcidPMC7586994-
dc.identifier.hkuros319483-
dc.identifier.volume10-
dc.identifier.issue6-
dc.identifier.spagearticle no. e190-
dc.identifier.epagearticle no. e190-
dc.identifier.eissn2001-1326-
dc.identifier.isiWOS:000588061700013-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2001-1326-

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