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Article: Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury

TitleHuman-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury
Authors
Wong, CKLuk, HKHLai, WHLau, YMZhang, RRWong, ACPLo, GCSChan, KHHung, IFNTse, HFWoo, PCYLau, SKPSiu, CW
KeywordsCOVID-19
Myocarditis
SARS-CoV-2
Stem cells
Issue Date2020
PublisherJapanese Circulation Society. The Journal's web site is located at http://www.j-circ.or.jp/english/publications/
Citation
Circulation Journal, 2020, v. 84 n. 11, p. 2027-2031 How to Cite?
DOI: http://dx.doi.org/10.1253/circj.CJ-20-0881
AbstractBackground: SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition. Methods and Results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated. Conclusions: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
Persistent Identifierhttp://hdl.handle.net/10722/294107
ISSN
1346-9843
2023 Impact Factor: 3.1
2023 SCImago Journal Rankings: 1.140
ISI Accession Number ID
WOS:000584484700020

 

DC FieldValueLanguage
dc.contributor.authorWong, CK-
dc.contributor.authorLuk, HKH-
dc.contributor.authorLai, WH-
dc.contributor.authorLau, YM-
dc.contributor.authorZhang, RR-
dc.contributor.authorWong, ACP-
dc.contributor.authorLo, GCS-
dc.contributor.authorChan, KH-
dc.contributor.authorHung, IFN-
dc.contributor.authorTse, HF-
dc.contributor.authorWoo, PCY-
dc.contributor.authorLau, SKP-
dc.contributor.authorSiu, CW-
dc.date.accessioned2020-11-23T08:26:25Z-
dc.date.available2020-11-23T08:26:25Z-
dc.date.issued2020-
dc.identifier.citationCirculation Journal, 2020, v. 84 n. 11, p. 2027-2031-
dc.identifier.issn1346-9843-
dc.identifier.urihttp://hdl.handle.net/10722/294107-
dc.description.abstractBackground: SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition. Methods and Results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated. Conclusions: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.-
dc.languageeng-
dc.publisherJapanese Circulation Society. The Journal's web site is located at http://www.j-circ.or.jp/english/publications/-
dc.relation.ispartofCirculation Journal-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCOVID-19-
dc.subjectMyocarditis-
dc.subjectSARS-CoV-2-
dc.subjectStem cells-
dc.titleHuman-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury-
dc.typeArticle-
dc.identifier.emailLuk, HKH: hkhluk@hku.hk-
dc.identifier.emailLai, WH: kwhlai@hku.hk-
dc.identifier.emailLau, YM: vymlau@hku.hk-
dc.identifier.emailLo, GCS: gcslo@connect.hku.hk-
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hk-
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hk-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hk-
dc.identifier.authorityChan, HKH=rp01921-
dc.identifier.authorityHung, IFN=rp00508-
dc.identifier.authorityTse, HF=rp00428-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authoritySiu, CW=rp00534-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1253/circj.CJ-20-0881-
dc.identifier.pmid32981925-
dc.identifier.scopuseid_2-s2.0-85093820936-
dc.identifier.hkuros319737-
dc.identifier.volume84-
dc.identifier.issue11-
dc.identifier.spage2027-
dc.identifier.epage2031-
dc.identifier.isiWOS:000584484700020-
dc.publisher.placeJapan-
dc.identifier.issnl1346-9843-

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