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Article: Phenotype and function of human natural killer cells purified by using a clinical-scale immunomagnetic method

TitlePhenotype and function of human natural killer cells purified by using a clinical-scale immunomagnetic method
Authors
KeywordsAdoptive immunotherapy
Bone marrow transplantation
Neuroblastoma
Natural killer cell
Antithymocyte globulin
Leukemia
Issue Date2005
Citation
Cancer Immunology, Immunotherapy, 2005, v. 54, n. 4, p. 389-394 How to Cite?
AbstractInfection, disease relapse, graft failure, and graft-versus-host disease (GVHD) are significant adverse events associated with allogeneic bone marrow transplantation. Donor natural killer (NK) cells may be an ideal cell type for prevention or treatment of all these adverse events. Therefore, we investigated the phenotype and function of human NK cells purified by using a clinical-scale immunomagnetic method. We found that the NK cell purification procedures did not adversely affect the expression of killer cell immunoglobulin-like receptors, adhesion molecules, intracellular cytokines, perforin, and granzyme B. Purified NK cells had extensive proliferative capacity and potent antitumor activity when assessed using an immunodeficient mouse model. While all mice transplanted with unpurified mononuclear cells developed GVHD, none of the mice transplanted with purified NK cells did. NK cells were highly susceptible to lysis by antithymocyte globulin (ATG), whereas G-CSF had a minimal effect on their natural cytotoxicity. These results support future clinical investigation of the use of purified NK cells for adoptive immunotherapy in the absence of ATG. © Springer-Verlag 2004.
Persistent Identifierhttp://hdl.handle.net/10722/294402
ISSN
2020 Impact Factor: 6.968
2020 SCImago Journal Rankings: 2.389
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, Wing-
dc.contributor.authorIyengar, Rekha-
dc.contributor.authorLeimig, Thasia-
dc.contributor.authorHolladay, Marti S.-
dc.contributor.authorHouston, James-
dc.contributor.authorHandgretinger, Rupert-
dc.date.accessioned2020-12-03T08:22:39Z-
dc.date.available2020-12-03T08:22:39Z-
dc.date.issued2005-
dc.identifier.citationCancer Immunology, Immunotherapy, 2005, v. 54, n. 4, p. 389-394-
dc.identifier.issn0340-7004-
dc.identifier.urihttp://hdl.handle.net/10722/294402-
dc.description.abstractInfection, disease relapse, graft failure, and graft-versus-host disease (GVHD) are significant adverse events associated with allogeneic bone marrow transplantation. Donor natural killer (NK) cells may be an ideal cell type for prevention or treatment of all these adverse events. Therefore, we investigated the phenotype and function of human NK cells purified by using a clinical-scale immunomagnetic method. We found that the NK cell purification procedures did not adversely affect the expression of killer cell immunoglobulin-like receptors, adhesion molecules, intracellular cytokines, perforin, and granzyme B. Purified NK cells had extensive proliferative capacity and potent antitumor activity when assessed using an immunodeficient mouse model. While all mice transplanted with unpurified mononuclear cells developed GVHD, none of the mice transplanted with purified NK cells did. NK cells were highly susceptible to lysis by antithymocyte globulin (ATG), whereas G-CSF had a minimal effect on their natural cytotoxicity. These results support future clinical investigation of the use of purified NK cells for adoptive immunotherapy in the absence of ATG. © Springer-Verlag 2004.-
dc.languageeng-
dc.relation.ispartofCancer Immunology, Immunotherapy-
dc.subjectAdoptive immunotherapy-
dc.subjectBone marrow transplantation-
dc.subjectNeuroblastoma-
dc.subjectNatural killer cell-
dc.subjectAntithymocyte globulin-
dc.subjectLeukemia-
dc.titlePhenotype and function of human natural killer cells purified by using a clinical-scale immunomagnetic method-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00262-004-0609-6-
dc.identifier.pmid15449041-
dc.identifier.scopuseid_2-s2.0-13744250567-
dc.identifier.volume54-
dc.identifier.issue4-
dc.identifier.spage389-
dc.identifier.epage394-
dc.identifier.isiWOS:000226799300011-
dc.identifier.issnl0340-7004-

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