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Article: Selection of stem cells by using antibodies that target different CD34 epitopes yields different patterns of T-cell differentiation

TitleSelection of stem cells by using antibodies that target different CD34 epitopes yields different patterns of T-cell differentiation
Authors
KeywordsCD34 progenitors
CD34 selection
Engraftment
Hematopoietic stem cell transplantation
Thymus
Immune reconstitution
T cell
Thymopoiesis
Issue Date2007
Citation
Stem Cells, 2007, v. 25, n. 2, p. 537-542 How to Cite?
AbstractThe objective of this study was to compare the patterns of T-cell differentiation from CD34+ human stem cells selected with different classes of antibody targeting the CD34 molecule. We compared signal-joint T-cell receptor excision circle (sjTREC) production in thymocytes selected with different classes of anti-CD34 antibody. Based on these results, we studied immune reconstitution in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice using human stem cells selected with the same antibodies that yielded variation in the thymocytes. Human CD34+ stem cells were immunomagnetically selected using the class II QBEnd antibody (prevalent in clinical graft engineering) and the class III 8G12 antibody (common in diagnostic tests). Engraftment and T-cell reconstitution were examined after transplantation. Thymocytes selected with the 8G12 class III antibody have a higher TREC production than those selected with the QBEnd class II antibody. Of mice transplanted with cells selected using the 8G12 antibody, 50% had sjTREC production, compared with 14% of mice transplanted with cells selected using the clinically common antibody QBEnd. 8G12 thymic progenitors are characterized by higher quality in thymic distribution and higher activity in T-cell differentiation. Using class III antibody targeting the CD34 molecule resulted in increased T-cell reconstitution in the NOD/SCID mouse. Use of a single antibody epitope targeting the CD34 molecule may lead to loss of cells that might provide richer T-cell reconstitution. Use of different or multiple epitopes, targeting of alternate stem cell markers, or use of cell-depletion strategies might prevent this loss. © 2007 AlphaMed Press.
Persistent Identifierhttp://hdl.handle.net/10722/294418
ISSN
2020 Impact Factor: 6.277
2020 SCImago Journal Rankings: 2.159
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorOtto, Mario-
dc.contributor.authorChen, Xiaohua-
dc.contributor.authorMartin, William J.-
dc.contributor.authorLeung, Wing-
dc.contributor.authorKnowles, James-
dc.contributor.authorHolladay, Marti-
dc.contributor.authorHouston, Jim-
dc.contributor.authorHandgretinger, Rupert-
dc.contributor.authorBarfield, Raymond C.-
dc.date.accessioned2020-12-03T08:22:41Z-
dc.date.available2020-12-03T08:22:41Z-
dc.date.issued2007-
dc.identifier.citationStem Cells, 2007, v. 25, n. 2, p. 537-542-
dc.identifier.issn1066-5099-
dc.identifier.urihttp://hdl.handle.net/10722/294418-
dc.description.abstractThe objective of this study was to compare the patterns of T-cell differentiation from CD34+ human stem cells selected with different classes of antibody targeting the CD34 molecule. We compared signal-joint T-cell receptor excision circle (sjTREC) production in thymocytes selected with different classes of anti-CD34 antibody. Based on these results, we studied immune reconstitution in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice using human stem cells selected with the same antibodies that yielded variation in the thymocytes. Human CD34+ stem cells were immunomagnetically selected using the class II QBEnd antibody (prevalent in clinical graft engineering) and the class III 8G12 antibody (common in diagnostic tests). Engraftment and T-cell reconstitution were examined after transplantation. Thymocytes selected with the 8G12 class III antibody have a higher TREC production than those selected with the QBEnd class II antibody. Of mice transplanted with cells selected using the 8G12 antibody, 50% had sjTREC production, compared with 14% of mice transplanted with cells selected using the clinically common antibody QBEnd. 8G12 thymic progenitors are characterized by higher quality in thymic distribution and higher activity in T-cell differentiation. Using class III antibody targeting the CD34 molecule resulted in increased T-cell reconstitution in the NOD/SCID mouse. Use of a single antibody epitope targeting the CD34 molecule may lead to loss of cells that might provide richer T-cell reconstitution. Use of different or multiple epitopes, targeting of alternate stem cell markers, or use of cell-depletion strategies might prevent this loss. © 2007 AlphaMed Press.-
dc.languageeng-
dc.relation.ispartofStem Cells-
dc.subjectCD34 progenitors-
dc.subjectCD34 selection-
dc.subjectEngraftment-
dc.subjectHematopoietic stem cell transplantation-
dc.subjectThymus-
dc.subjectImmune reconstitution-
dc.subjectT cell-
dc.subjectThymopoiesis-
dc.titleSelection of stem cells by using antibodies that target different CD34 epitopes yields different patterns of T-cell differentiation-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1634/stemcells.2006-0319-
dc.identifier.pmid17023516-
dc.identifier.scopuseid_2-s2.0-33846901843-
dc.identifier.volume25-
dc.identifier.issue2-
dc.identifier.spage537-
dc.identifier.epage542-
dc.identifier.isiWOS:000244070600033-
dc.identifier.issnl1066-5099-

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