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Article: Multiplex and genome-wide analyses reveal distinctive properties of KIR+ and CD56+ T cells in human blood

TitleMultiplex and genome-wide analyses reveal distinctive properties of KIR<sup>+</sup> and CD56<sup>+</sup> T cells in human blood
Authors
Issue Date2013
Citation
Journal of Immunology, 2013, v. 191, n. 4, p. 1625-1636 How to Cite?
AbstractKiller cell Ig-like receptors (KIRs) on NK cells have been linked to a wide spectrum of health conditions such as chronic infections, autoimmune diseases, pregnancy complications, cancers, and transplant failures. A small subset of effector memory T cells also expresses KIRs. In this study, we use modern analytic tools including genome-wide and multiplex molecular, phenotypic, and functional assays to characterize the KIR+ T cells in human blood. We find that KIR+ T cells primarily reside in the CD56+ T population that is distinctively DNAM-1high with a genome-wide quiescent transcriptome, short telomere, and limited TCR excision circles. During CMV reactivation in bone marrow transplant recipients, KIR+CD56 + T cells rapidly expanded in realtime but not KIR+CD562 T cells or KIR+ NK cells. In CMV+ asymptomatic donors, as much as 50% of CD56+ T cells are KIR+, and most are distinguishably KIR2DL2/3+NKG2C+CD57+. Functionally, the KIR+CD56+ T cell subset lyses cancer cells and CMVpp65-pulsed target cells in a dual KIR-dependent and TCR-dependent manner. Analysis of metabolic transcriptome confirms the immunological memory status of KIR+CD56+ T cells in contrast to KIR -CD56+ T cells that are more active in energy metabolism and effector differentiation. KIR-CD56+ T cells have >25-fold higher level of expression of RORC than the KIR+ counterpart and are a previously unknown producer of IL-13 rather than IL-17 in multiplex cytokine arrays. Our data provide fundamental insights into KIR+ T cells biologically and clinically. © 2013 by The American Association of Immunologists, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/294468
ISSN
2020 Impact Factor: 5.422
2020 SCImago Journal Rankings: 2.737
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Wing Keung-
dc.contributor.authorRujkijyanont, Piya-
dc.contributor.authorNeale, Geoffrey-
dc.contributor.authorYang, Jie-
dc.contributor.authorBari, Rafijul-
dc.contributor.authorGupta, Neha Das-
dc.contributor.authorHolladay, Martha-
dc.contributor.authorRooney, Barbara-
dc.contributor.authorLeung, Wing-
dc.date.accessioned2020-12-03T08:22:48Z-
dc.date.available2020-12-03T08:22:48Z-
dc.date.issued2013-
dc.identifier.citationJournal of Immunology, 2013, v. 191, n. 4, p. 1625-1636-
dc.identifier.issn0022-1767-
dc.identifier.urihttp://hdl.handle.net/10722/294468-
dc.description.abstractKiller cell Ig-like receptors (KIRs) on NK cells have been linked to a wide spectrum of health conditions such as chronic infections, autoimmune diseases, pregnancy complications, cancers, and transplant failures. A small subset of effector memory T cells also expresses KIRs. In this study, we use modern analytic tools including genome-wide and multiplex molecular, phenotypic, and functional assays to characterize the KIR+ T cells in human blood. We find that KIR+ T cells primarily reside in the CD56+ T population that is distinctively DNAM-1high with a genome-wide quiescent transcriptome, short telomere, and limited TCR excision circles. During CMV reactivation in bone marrow transplant recipients, KIR+CD56 + T cells rapidly expanded in realtime but not KIR+CD562 T cells or KIR+ NK cells. In CMV+ asymptomatic donors, as much as 50% of CD56+ T cells are KIR+, and most are distinguishably KIR2DL2/3+NKG2C+CD57+. Functionally, the KIR+CD56+ T cell subset lyses cancer cells and CMVpp65-pulsed target cells in a dual KIR-dependent and TCR-dependent manner. Analysis of metabolic transcriptome confirms the immunological memory status of KIR+CD56+ T cells in contrast to KIR -CD56+ T cells that are more active in energy metabolism and effector differentiation. KIR-CD56+ T cells have >25-fold higher level of expression of RORC than the KIR+ counterpart and are a previously unknown producer of IL-13 rather than IL-17 in multiplex cytokine arrays. Our data provide fundamental insights into KIR+ T cells biologically and clinically. © 2013 by The American Association of Immunologists, Inc.-
dc.languageeng-
dc.relation.ispartofJournal of Immunology-
dc.titleMultiplex and genome-wide analyses reveal distinctive properties of KIR<sup>+</sup> and CD56<sup>+</sup> T cells in human blood-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4049/jimmunol.1300111-
dc.identifier.pmid23858032-
dc.identifier.pmcidPMC4275795-
dc.identifier.scopuseid_2-s2.0-84881450402-
dc.identifier.volume191-
dc.identifier.issue4-
dc.identifier.spage1625-
dc.identifier.epage1636-
dc.identifier.eissn1550-6606-
dc.identifier.isiWOS:000322632900013-
dc.identifier.issnl0022-1767-

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