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Article: Enhanced Cytotoxic Function of Natural Killer and CD3+CD56+ Cells in Cord Blood after Culture

TitleEnhanced Cytotoxic Function of Natural Killer and CD3<sup>+</sup>CD56<sup>+</sup> Cells in Cord Blood after Culture
Authors
KeywordsCytotoxicity
Natural killer
Cord blood
Killer cell immunoglobulin-like receptors (KIR)
Exvivo expansion
Issue Date2015
Citation
Biology of Blood and Marrow Transplantation, 2015, v. 21, n. 1, p. 39-49 How to Cite?
Abstract© 2015 American Society for Blood and Marrow Transplantation. Rate of immune reconstitution directly correlates with the number of hematopoietic stem cells infused and is particularly delayed in patients undergoing cord blood (CB) transplantation (CBT). Methods to increase the number of CB natural killer (NK) cells have the potential to improve immune reconstitution after CBT. NK cells are the first lymphocyte population to recover after hematopoietic stem cells transplantation and are central to preventing early relapse and infection. CB NK cells are low in number and are known to be incomplete in maturation and require activation for effective function. Here, we report a clinically relevant exvivo expansion method that increases the number of activated CB NK cells. We report a multilog increase in NK cell number when CB mononuclear cells are cocultured with IL-2 and IL-15. Furthermore, NK cells expressing activating receptors and adhesion molecules responsible for cytotoxicity increased throughout culture, whereas inhibitory receptor expression remained low. Additionally, cytotoxic function against various malignancies was significantly enhanced in cultured NK cells but not CD3+CD56+ cells. These data suggest that exvivo expansion and activation of CB NK cells is a clinically feasible and relevant approach to prevent early infection and relapse after CBT.
Persistent Identifierhttp://hdl.handle.net/10722/294479
ISSN
2020 Impact Factor: 5.742
2015 SCImago Journal Rankings: 2.094
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTomchuck, Suzanne L.-
dc.contributor.authorLeung, Wing H.-
dc.contributor.authorDallas, Mari H.-
dc.date.accessioned2020-12-03T08:22:50Z-
dc.date.available2020-12-03T08:22:50Z-
dc.date.issued2015-
dc.identifier.citationBiology of Blood and Marrow Transplantation, 2015, v. 21, n. 1, p. 39-49-
dc.identifier.issn1083-8791-
dc.identifier.urihttp://hdl.handle.net/10722/294479-
dc.description.abstract© 2015 American Society for Blood and Marrow Transplantation. Rate of immune reconstitution directly correlates with the number of hematopoietic stem cells infused and is particularly delayed in patients undergoing cord blood (CB) transplantation (CBT). Methods to increase the number of CB natural killer (NK) cells have the potential to improve immune reconstitution after CBT. NK cells are the first lymphocyte population to recover after hematopoietic stem cells transplantation and are central to preventing early relapse and infection. CB NK cells are low in number and are known to be incomplete in maturation and require activation for effective function. Here, we report a clinically relevant exvivo expansion method that increases the number of activated CB NK cells. We report a multilog increase in NK cell number when CB mononuclear cells are cocultured with IL-2 and IL-15. Furthermore, NK cells expressing activating receptors and adhesion molecules responsible for cytotoxicity increased throughout culture, whereas inhibitory receptor expression remained low. Additionally, cytotoxic function against various malignancies was significantly enhanced in cultured NK cells but not CD3+CD56+ cells. These data suggest that exvivo expansion and activation of CB NK cells is a clinically feasible and relevant approach to prevent early infection and relapse after CBT.-
dc.languageeng-
dc.relation.ispartofBiology of Blood and Marrow Transplantation-
dc.subjectCytotoxicity-
dc.subjectNatural killer-
dc.subjectCord blood-
dc.subjectKiller cell immunoglobulin-like receptors (KIR)-
dc.subjectExvivo expansion-
dc.titleEnhanced Cytotoxic Function of Natural Killer and CD3<sup>+</sup>CD56<sup>+</sup> Cells in Cord Blood after Culture-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.bbmt.2014.10.014-
dc.identifier.pmid25464114-
dc.identifier.pmcidPMC4700822-
dc.identifier.scopuseid_2-s2.0-84927154659-
dc.identifier.volume21-
dc.identifier.issue1-
dc.identifier.spage39-
dc.identifier.epage49-
dc.identifier.eissn1523-6536-
dc.identifier.isiWOS:000347671600008-
dc.identifier.issnl1083-8791-

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