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- Publisher Website: 10.1007/s10544-020-00538-9
- Scopus: eid_2-s2.0-85098853941
- PMID: 33415464
- WOS: WOS:000608017700002
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Article: Therapeutic approach for global myocardial injury using bone marrow-derived mesenchymal stem cells by cardiac support device in rats
Title | Therapeutic approach for global myocardial injury using bone marrow-derived mesenchymal stem cells by cardiac support device in rats |
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Authors | |
Keywords | Global myocardial injury BMSCs Active cardiac support device (ASD) Stem cell treatment Epicardial delivery |
Issue Date | 2021 |
Publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1387-2176 |
Citation | Biomedical Microdevices, 2021, v. 23 n. 1, p. article no. 5 How to Cite? |
Abstract | Bone marrow-derived mesenchymal stem cells (BMSCs) have been considered a promising therapeutic approach to cardiovascular disease. This study intends to compare the effect of BMSCs through a standard active cardiac support device (ASD) and intravenous injection on global myocardial injury induced by isoproterenol. BMSCs were cultured in vitro, and the transplanted cells were labeled with a fluorescent dye CM-Dil. Isoproterenol (ISO) was injected into the rats; 2 weeks later, the labeled cells were transplanted into ISO-induced heart-jury rats through the tail vein or ASD device for 5 days. The rats were sacrificed on the first day, the third day, and the fifth day after transplantation to observe the distribution of cells in the myocardium by fluorescence microscopy. The hemodynamic indexes of the left ventricle were measured before sacrificing. H&E staining and Masson’s trichrome staining were used to evaluate the cardiac histopathology. In the ASD groups, after 3 days of transplantation, there were a large number of BMSCs on the epicardial surface, and after 5 days of transplantation, BMSCs were widely distributed in the ventricular muscle. But in the intravenous injection group, there were no labeled-BMSCs distributed. In the ASD + BMSCs-three days treated group and ASD + BMSCs -five days-treated group, left ventricular systolic pressure (LVSP), the maximum rate of left ventricular pressure rise (+dP/dt), the maximum rate of left ventricular pressure decline (-dP/dt) increased compared with model group and intravenous injection group (P < 0.05). By giving BMSCs through ASD device, cells can rapidly and widely distribute in the myocardium and significantly improve heart function. |
Persistent Identifier | http://hdl.handle.net/10722/295248 |
ISSN | 2023 Impact Factor: 3.0 2023 SCImago Journal Rankings: 0.578 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liu, Z | - |
dc.contributor.author | Naveed, M | - |
dc.contributor.author | Baig, MMFA | - |
dc.contributor.author | Mikrani, R | - |
dc.contributor.author | Li, C | - |
dc.contributor.author | Saeed, M | - |
dc.contributor.author | Zhang, Q | - |
dc.contributor.author | Farooq, MA | - |
dc.contributor.author | Zubair, HM | - |
dc.contributor.author | Zhou, X | - |
dc.date.accessioned | 2021-01-11T13:57:25Z | - |
dc.date.available | 2021-01-11T13:57:25Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Biomedical Microdevices, 2021, v. 23 n. 1, p. article no. 5 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295248 | - |
dc.description.abstract | Bone marrow-derived mesenchymal stem cells (BMSCs) have been considered a promising therapeutic approach to cardiovascular disease. This study intends to compare the effect of BMSCs through a standard active cardiac support device (ASD) and intravenous injection on global myocardial injury induced by isoproterenol. BMSCs were cultured in vitro, and the transplanted cells were labeled with a fluorescent dye CM-Dil. Isoproterenol (ISO) was injected into the rats; 2 weeks later, the labeled cells were transplanted into ISO-induced heart-jury rats through the tail vein or ASD device for 5 days. The rats were sacrificed on the first day, the third day, and the fifth day after transplantation to observe the distribution of cells in the myocardium by fluorescence microscopy. The hemodynamic indexes of the left ventricle were measured before sacrificing. H&E staining and Masson’s trichrome staining were used to evaluate the cardiac histopathology. In the ASD groups, after 3 days of transplantation, there were a large number of BMSCs on the epicardial surface, and after 5 days of transplantation, BMSCs were widely distributed in the ventricular muscle. But in the intravenous injection group, there were no labeled-BMSCs distributed. In the ASD + BMSCs-three days treated group and ASD + BMSCs -five days-treated group, left ventricular systolic pressure (LVSP), the maximum rate of left ventricular pressure rise (+dP/dt), the maximum rate of left ventricular pressure decline (-dP/dt) increased compared with model group and intravenous injection group (P < 0.05). By giving BMSCs through ASD device, cells can rapidly and widely distribute in the myocardium and significantly improve heart function. | - |
dc.language | eng | - |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1387-2176 | - |
dc.relation.ispartof | Biomedical Microdevices | - |
dc.rights | This is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI] | - |
dc.subject | Global myocardial injury | - |
dc.subject | BMSCs | - |
dc.subject | Active cardiac support device (ASD) | - |
dc.subject | Stem cell treatment | - |
dc.subject | Epicardial delivery | - |
dc.title | Therapeutic approach for global myocardial injury using bone marrow-derived mesenchymal stem cells by cardiac support device in rats | - |
dc.type | Article | - |
dc.identifier.email | Baig, MMFA: faran@HKUCC-COM.hku.hk | - |
dc.identifier.authority | Baig, MMFA=rp02755 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s10544-020-00538-9 | - |
dc.identifier.pmid | 33415464 | - |
dc.identifier.scopus | eid_2-s2.0-85098853941 | - |
dc.identifier.hkuros | 320893 | - |
dc.identifier.volume | 23 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 5 | - |
dc.identifier.epage | article no. 5 | - |
dc.identifier.isi | WOS:000608017700002 | - |
dc.publisher.place | United States | - |