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Article: Hemogenic endothelium differentiation from human pluripotent stem cells in a feeder- and xeno-free defined condition
Title | Hemogenic endothelium differentiation from human pluripotent stem cells in a feeder- and xeno-free defined condition |
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Authors | |
Keywords | Issue 148 Human pluripotent stem cells Directed differentiation Feeder-free Xeno-free Hemogenic endothelium Mesoderm organoids Hematopoiesis Developmental Biology PSC spheroids |
Issue Date | 2019 |
Citation | Journal of Visualized Experiments, 2019, n. 148, article no. e59823 How to Cite? |
Abstract | Deriving functional hematopoietic stem and progenitor cells (HSPCs) from human pluripotent stem cells (PSCs) have been an elusive goal for autologous transplants to treat hematological and malignant disorders. Hemogenic endothelium (HE) is an amenable platform to produce functional HSPCs by transcription factors or to induce lymphocytes in a robust manner. However, current methods to derive HE are either costly or variable in yield. In this protocol, we established a cost-effective and precise method to derive HE within 4 days in a feeder-and xeno-free defined condition. The resultant HE underwent an endothelial-to-hematopoietic transition and produced CD34+CD45+ clonogenic hematopoietic progenitors. The potential capacity of our platform for generating functional HSPCs will have broad applications in disease modeling and screening for therapeutic compounds. |
Persistent Identifier | http://hdl.handle.net/10722/295414 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.449 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ohta, Ryo | - |
dc.contributor.author | Sugimura, Ryohichi | - |
dc.contributor.author | Niwa, Akira | - |
dc.contributor.author | Saito, Megumu K. | - |
dc.date.accessioned | 2021-01-18T15:46:49Z | - |
dc.date.available | 2021-01-18T15:46:49Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Journal of Visualized Experiments, 2019, n. 148, article no. e59823 | - |
dc.identifier.issn | 1940-087X | - |
dc.identifier.uri | http://hdl.handle.net/10722/295414 | - |
dc.description.abstract | Deriving functional hematopoietic stem and progenitor cells (HSPCs) from human pluripotent stem cells (PSCs) have been an elusive goal for autologous transplants to treat hematological and malignant disorders. Hemogenic endothelium (HE) is an amenable platform to produce functional HSPCs by transcription factors or to induce lymphocytes in a robust manner. However, current methods to derive HE are either costly or variable in yield. In this protocol, we established a cost-effective and precise method to derive HE within 4 days in a feeder-and xeno-free defined condition. The resultant HE underwent an endothelial-to-hematopoietic transition and produced CD34+CD45+ clonogenic hematopoietic progenitors. The potential capacity of our platform for generating functional HSPCs will have broad applications in disease modeling and screening for therapeutic compounds. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Visualized Experiments | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Issue 148 | - |
dc.subject | Human pluripotent stem cells | - |
dc.subject | Directed differentiation | - |
dc.subject | Feeder-free | - |
dc.subject | Xeno-free | - |
dc.subject | Hemogenic endothelium | - |
dc.subject | Mesoderm organoids | - |
dc.subject | Hematopoiesis | - |
dc.subject | Developmental Biology | - |
dc.subject | PSC spheroids | - |
dc.title | Hemogenic endothelium differentiation from human pluripotent stem cells in a feeder- and xeno-free defined condition | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3791/59823 | - |
dc.identifier.pmid | 31259914 | - |
dc.identifier.scopus | eid_2-s2.0-85068964078 | - |
dc.identifier.issue | 148 | - |
dc.identifier.spage | article no. e59823 | - |
dc.identifier.epage | article no. e59823 | - |
dc.identifier.isi | WOS:000473295900110 | - |
dc.identifier.issnl | 1940-087X | - |