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- Publisher Website: 10.1007/s10544-020-00488-2
- Scopus: eid_2-s2.0-85084184629
- PMID: 32377802
- WOS: WOS:000530797700001
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Article: Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis
Title | Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis |
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Authors | |
Keywords | Pluripotent stem cells Organs-on-chips Hematopoietic stem and progenitor cells Hematopoiesis |
Issue Date | 2020 |
Citation | Biomedical Microdevices, 2020, v. 22, n. 2, article no. 34 How to Cite? |
Abstract | © 2020, Springer Science+Business Media, LLC, part of Springer Nature. A fundamental limitation in the derivation of hematopoietic stem and progenitor cells is the imprecise understanding of human developmental hematopoiesis. Herein we established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from pluripotent stem cells. The device consists of two layers of microchannels separated by a semipermeable membrane, which allows the co-culture of human hemogenic endothelial (HE) cells and stromal cells in a physiological relevant spatial arrangement to replicate the structure of the AGM. HE cells derived from human induced pluripotent stem cells (hiPSCs) were cultured on a layer of mesenchymal stromal cells in the top channel while vascular endothelial cells were co-cultured on the bottom side of the membrane within the microfluidic device. We show that this AGM-on-a-chip efficiently derives endothelial-to-hematopoietic transition (EHT) from hiPSCs compared with regular suspension culture. The presence of mesenchymal stroma and endothelial cells renders functional HPCs in vitro. We propose that the AGM-on-a-chip could serve as a platform to dissect the cellular and molecular mechanisms of human developmental hematopoiesis. |
Persistent Identifier | http://hdl.handle.net/10722/295423 |
ISSN | 2023 Impact Factor: 3.0 2023 SCImago Journal Rankings: 0.578 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Sugimura, Ryohichi | - |
dc.contributor.author | Ohta, Ryo | - |
dc.contributor.author | Mori, Chihiro | - |
dc.contributor.author | Li, Alina | - |
dc.contributor.author | Mano, Takafumi | - |
dc.contributor.author | Sano, Emi | - |
dc.contributor.author | Kosugi, Kaori | - |
dc.contributor.author | Nakahata, Tatsutoshi | - |
dc.contributor.author | Niwa, Akira | - |
dc.contributor.author | Saito, Megumu K. | - |
dc.contributor.author | Torisawa, Yu suke | - |
dc.date.accessioned | 2021-01-18T15:46:50Z | - |
dc.date.available | 2021-01-18T15:46:50Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Biomedical Microdevices, 2020, v. 22, n. 2, article no. 34 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295423 | - |
dc.description.abstract | © 2020, Springer Science+Business Media, LLC, part of Springer Nature. A fundamental limitation in the derivation of hematopoietic stem and progenitor cells is the imprecise understanding of human developmental hematopoiesis. Herein we established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from pluripotent stem cells. The device consists of two layers of microchannels separated by a semipermeable membrane, which allows the co-culture of human hemogenic endothelial (HE) cells and stromal cells in a physiological relevant spatial arrangement to replicate the structure of the AGM. HE cells derived from human induced pluripotent stem cells (hiPSCs) were cultured on a layer of mesenchymal stromal cells in the top channel while vascular endothelial cells were co-cultured on the bottom side of the membrane within the microfluidic device. We show that this AGM-on-a-chip efficiently derives endothelial-to-hematopoietic transition (EHT) from hiPSCs compared with regular suspension culture. The presence of mesenchymal stroma and endothelial cells renders functional HPCs in vitro. We propose that the AGM-on-a-chip could serve as a platform to dissect the cellular and molecular mechanisms of human developmental hematopoiesis. | - |
dc.language | eng | - |
dc.relation.ispartof | Biomedical Microdevices | - |
dc.subject | Pluripotent stem cells | - |
dc.subject | Organs-on-chips | - |
dc.subject | Hematopoietic stem and progenitor cells | - |
dc.subject | Hematopoiesis | - |
dc.title | Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s10544-020-00488-2 | - |
dc.identifier.pmid | 32377802 | - |
dc.identifier.scopus | eid_2-s2.0-85084184629 | - |
dc.identifier.volume | 22 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | article no. 34 | - |
dc.identifier.epage | article no. 34 | - |
dc.identifier.eissn | 1572-8781 | - |
dc.identifier.isi | WOS:000530797700001 | - |
dc.identifier.issnl | 1387-2176 | - |