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Article: Proper positioning of mice for Cobb angle radiographic measurements
Title | Proper positioning of mice for Cobb angle radiographic measurements |
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Authors | |
Keywords | Scoliosis Mouse Radiograph Cobb angle Positioning |
Issue Date | 2021 |
Publisher | BioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmusculoskeletdisord/ |
Citation | BMC Musculoskeletal Disorders, 2021, v. 22 n. 1, p. article no. 72 How to Cite? |
Abstract | BackgroundThere is no recommended standard for positioning of a mouse for radiographic assessment of the spine. This is necessary to have reproducible radiographic data and avoid false positive results. The objective of this study was to investigate the impact of various postures on Cobb angle measurements and to set up a positioning standard for imaging mouse spines.MethodsThis study was conducted in three parts. Firstly, we identified the problem of lack of posture standardization for radiographs. We collected 77 C57BL/6J mice for spine radiographs and found a scoliosis prevalence of 28.6% with large variations in curve magnitude. Secondly, 24 C57BL/6J mice underwent 4 consecutive weekly radiographs and observed high variations (relative standard deviation: 125.3%) between radiographs. Thirdly, we collected another 82 C57BL/6J mice and designed 14 different postures that could take place during imaging. These postures were related to curling of the limbs, and head, pelvic and tail tilting.ResultsThe results showed that head and pelvic tilting significantly affects the curve magnitude with effect size (Glass's delta) over 1.50. Avoiding these incorrect positions during radiographs is warranted. The standard recommended posture for mouse imaging entails positioning the snout, interorbital space, neck and whole spine in one line, and with the limbs placed symmetrical to the trunk, whilst avoiding stretching the body of the mouse.ConclusionsOur work exemplified the importance of standard protocol during imaging when using an animal model in the scoliosis study. We recommend utilizing this standard in studying various disorders of the spine to avoid technical causes for the appearance of a curve. |
Persistent Identifier | http://hdl.handle.net/10722/295766 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.714 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | CHEN, Z | - |
dc.contributor.author | Luk, KDK | - |
dc.contributor.author | Song, YQ | - |
dc.contributor.author | Gao, B | - |
dc.contributor.author | Cheung, JPY | - |
dc.date.accessioned | 2021-02-08T08:13:42Z | - |
dc.date.available | 2021-02-08T08:13:42Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | BMC Musculoskeletal Disorders, 2021, v. 22 n. 1, p. article no. 72 | - |
dc.identifier.issn | 1471-2474 | - |
dc.identifier.uri | http://hdl.handle.net/10722/295766 | - |
dc.description.abstract | BackgroundThere is no recommended standard for positioning of a mouse for radiographic assessment of the spine. This is necessary to have reproducible radiographic data and avoid false positive results. The objective of this study was to investigate the impact of various postures on Cobb angle measurements and to set up a positioning standard for imaging mouse spines.MethodsThis study was conducted in three parts. Firstly, we identified the problem of lack of posture standardization for radiographs. We collected 77 C57BL/6J mice for spine radiographs and found a scoliosis prevalence of 28.6% with large variations in curve magnitude. Secondly, 24 C57BL/6J mice underwent 4 consecutive weekly radiographs and observed high variations (relative standard deviation: 125.3%) between radiographs. Thirdly, we collected another 82 C57BL/6J mice and designed 14 different postures that could take place during imaging. These postures were related to curling of the limbs, and head, pelvic and tail tilting.ResultsThe results showed that head and pelvic tilting significantly affects the curve magnitude with effect size (Glass's delta) over 1.50. Avoiding these incorrect positions during radiographs is warranted. The standard recommended posture for mouse imaging entails positioning the snout, interorbital space, neck and whole spine in one line, and with the limbs placed symmetrical to the trunk, whilst avoiding stretching the body of the mouse.ConclusionsOur work exemplified the importance of standard protocol during imaging when using an animal model in the scoliosis study. We recommend utilizing this standard in studying various disorders of the spine to avoid technical causes for the appearance of a curve. | - |
dc.language | eng | - |
dc.publisher | BioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmusculoskeletdisord/ | - |
dc.relation.ispartof | BMC Musculoskeletal Disorders | - |
dc.rights | BMC Musculoskeletal Disorders. Copyright © BioMed Central Ltd. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Scoliosis | - |
dc.subject | Mouse | - |
dc.subject | Radiograph | - |
dc.subject | Cobb angle | - |
dc.subject | Positioning | - |
dc.title | Proper positioning of mice for Cobb angle radiographic measurements | - |
dc.type | Article | - |
dc.identifier.email | Song, YQ: songy@hku.hk | - |
dc.identifier.email | Gao, B: gaobo@hku.hk | - |
dc.identifier.email | Cheung, JPY: cheungjp@hku.hk | - |
dc.identifier.authority | Luk, KDK=rp00333 | - |
dc.identifier.authority | Song, YQ=rp00488 | - |
dc.identifier.authority | Gao, B=rp02012 | - |
dc.identifier.authority | Cheung, JPY=rp01685 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1186/s12891-021-03949-8 | - |
dc.identifier.pmid | 33435951 | - |
dc.identifier.pmcid | PMC7805199 | - |
dc.identifier.scopus | eid_2-s2.0-85099249392 | - |
dc.identifier.hkuros | 321140 | - |
dc.identifier.volume | 22 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 72 | - |
dc.identifier.epage | article no. 72 | - |
dc.identifier.isi | WOS:000609614000007 | - |
dc.publisher.place | United Kingdom | - |