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Article: Single-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells

TitleSingle-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells
Authors
KeywordsCD9
pancreatic β cells
single-cell transcriptomics
cell-surface marker
human pluripotent stem cells
Issue Date2020
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://stemcellreports.cell.com
Citation
Stem Cell Reports, 2020, v. 15 n. 5, p. 1111-1126 How to Cite?
AbstractSummary To date, it remains unclear if there are specific cell-surface markers for purifying glucose-responsive pancreatic β-like cells derived from human pluripotent stem cells (hPSCs). In searching for this, we generated an efficient protocol for differentiating β-like cells from human embryonic stem cells. We performed single-cell RNA sequencing and found that CD9 is a negative cell-surface marker of β-like cells, as most INS+ cells are CD9−. We purified β-like cells for spontaneous formation of islet-like organoids against CD9, and found significantly more NKX6.1+MAFA+C-PEPTIDE+ β-like cells in the CD9− than in the CD9+ population. CD9− cells also demonstrate better glucose responsiveness than CD9+ cells. In humans, we observe more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Taken together, our experiments show that CD9 is a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from hPSCs.
Persistent Identifierhttp://hdl.handle.net/10722/295775
ISSN
2020 Impact Factor: 7.765
2020 SCImago Journal Rankings: 3.207
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, X-
dc.contributor.authorYang, KY-
dc.contributor.authorChan, VW-
dc.contributor.authorLeung, KT-
dc.contributor.authorZhang, XB-
dc.contributor.authorWong, AS-
dc.contributor.authorChong, CCN-
dc.contributor.authorWang, CC-
dc.contributor.authorKu, MC-
dc.contributor.authorLui, KO-
dc.date.accessioned2021-02-08T08:13:50Z-
dc.date.available2021-02-08T08:13:50Z-
dc.date.issued2020-
dc.identifier.citationStem Cell Reports, 2020, v. 15 n. 5, p. 1111-1126-
dc.identifier.issn2213-6711-
dc.identifier.urihttp://hdl.handle.net/10722/295775-
dc.description.abstractSummary To date, it remains unclear if there are specific cell-surface markers for purifying glucose-responsive pancreatic β-like cells derived from human pluripotent stem cells (hPSCs). In searching for this, we generated an efficient protocol for differentiating β-like cells from human embryonic stem cells. We performed single-cell RNA sequencing and found that CD9 is a negative cell-surface marker of β-like cells, as most INS+ cells are CD9−. We purified β-like cells for spontaneous formation of islet-like organoids against CD9, and found significantly more NKX6.1+MAFA+C-PEPTIDE+ β-like cells in the CD9− than in the CD9+ population. CD9− cells also demonstrate better glucose responsiveness than CD9+ cells. In humans, we observe more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Taken together, our experiments show that CD9 is a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from hPSCs.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://stemcellreports.cell.com-
dc.relation.ispartofStem Cell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCD9-
dc.subjectpancreatic β cells-
dc.subjectsingle-cell transcriptomics-
dc.subjectcell-surface marker-
dc.subjecthuman pluripotent stem cells-
dc.titleSingle-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells-
dc.typeArticle-
dc.identifier.emailWong, AS: aslw@hku.hk-
dc.identifier.authorityWong, AS=rp02139-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.stemcr.2020.09.009-
dc.identifier.pmid33096048-
dc.identifier.pmcidPMC7663789-
dc.identifier.scopuseid_2-s2.0-85096151717-
dc.identifier.hkuros321228-
dc.identifier.volume15-
dc.identifier.issue5-
dc.identifier.spage1111-
dc.identifier.epage1126-
dc.identifier.isiWOS:000590837600009-
dc.publisher.placeUnited States-

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