File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Book Chapter: Detection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques

TitleDetection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques
Authors
KeywordsIL-10
Regulatory B cells
Adoptive transfer
In situ detection
Tile scan
Issue Date2021
PublisherHumana
Citation
Detection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques. In Mion, F & Tonon, S (Eds.), Regulatory B Cells, p. 93-111. New York, NY: Humana, 2021 How to Cite?
AbstractWith the ever-increasing understanding of the roles of B cells in immune response and autoimmune pathogenesis, various techniques have been optimized for the detection of IL-10 production in B cells. In this chapter, we describe several commonly used methods for the effective detection of IL-10 in B cells at both mRNA and protein levels, including quantitative PCR analysis, intracellular staining of IL-10 in live B cells by flow cytometry, ELISA for secreted IL-10 detection, and ELISPOT assay for enumerating IL-10-producing B cells. We have further co-stained IL-10 with other cytokines and examined the staining efficiency. Moreover, we provide a detailed protocol for the detection of IL-10-producing B cells in situ by immunofluorescence microscopy. Since emerging evidence has suggested the promising strategy of cell therapy, we also provide a protocol to determine CD19+CD1dhiCD5+ B-cell distribution upon adoptive transfer using tile-scan imaging. Together, the application of the described methods for the detection of IL-10 will facilitate the characterization of B-cell subsets with regulatory functions and enhance our current understanding of the critical roles of B cells in immune response and autoimmune development.
Persistent Identifierhttp://hdl.handle.net/10722/295821
ISBN
Series/Report no.Methods in Molecular Biology (MIMB) ; v. 2270

 

DC FieldValueLanguage
dc.contributor.authorLin, X-
dc.contributor.authorWang, X-
dc.contributor.authorLu, L-
dc.date.accessioned2021-02-08T08:14:29Z-
dc.date.available2021-02-08T08:14:29Z-
dc.date.issued2021-
dc.identifier.citationDetection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques. In Mion, F & Tonon, S (Eds.), Regulatory B Cells, p. 93-111. New York, NY: Humana, 2021-
dc.identifier.isbn9781071612361-
dc.identifier.urihttp://hdl.handle.net/10722/295821-
dc.description.abstractWith the ever-increasing understanding of the roles of B cells in immune response and autoimmune pathogenesis, various techniques have been optimized for the detection of IL-10 production in B cells. In this chapter, we describe several commonly used methods for the effective detection of IL-10 in B cells at both mRNA and protein levels, including quantitative PCR analysis, intracellular staining of IL-10 in live B cells by flow cytometry, ELISA for secreted IL-10 detection, and ELISPOT assay for enumerating IL-10-producing B cells. We have further co-stained IL-10 with other cytokines and examined the staining efficiency. Moreover, we provide a detailed protocol for the detection of IL-10-producing B cells in situ by immunofluorescence microscopy. Since emerging evidence has suggested the promising strategy of cell therapy, we also provide a protocol to determine CD19+CD1dhiCD5+ B-cell distribution upon adoptive transfer using tile-scan imaging. Together, the application of the described methods for the detection of IL-10 will facilitate the characterization of B-cell subsets with regulatory functions and enhance our current understanding of the critical roles of B cells in immune response and autoimmune development.-
dc.languageeng-
dc.publisherHumana-
dc.relation.ispartofRegulatory B Cells-
dc.relation.ispartofseriesMethods in Molecular Biology (MIMB) ; v. 2270-
dc.subjectIL-10-
dc.subjectRegulatory B cells-
dc.subjectAdoptive transfer-
dc.subjectIn situ detection-
dc.subjectTile scan-
dc.titleDetection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniques-
dc.typeBook_Chapter-
dc.identifier.emailLin, X: linxiang@hku.hk-
dc.identifier.emailWang, X: xiaohuiwang@hku.hk-
dc.identifier.emailLu, L: liweilu@hku.hk-
dc.identifier.authorityLin, X=rp02623-
dc.identifier.authorityWang, X=rp02664-
dc.identifier.authorityLu, L=rp00477-
dc.identifier.doi10.1007/978-1-0716-1237-8_6-
dc.identifier.pmid33479895-
dc.identifier.hkuros321194-
dc.identifier.spage93-
dc.identifier.epage111-
dc.publisher.placeNew York, NY-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats