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Article: Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test

TitleRandomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
Authors
KeywordsNanoplasmonics
Plasmon-enhanced fluorescence
Photochemical immobilization technique
Antibody-aptamer biosensor
Malaria marker
Issue Date2021
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/chemistry/journal/604
Citation
Microchimica Acta, 2021, v. 188 n. 3, p. article no. 88 How to Cite?
AbstractA plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays.
DescriptionHybrid open access
Persistent Identifierhttp://hdl.handle.net/10722/297142
ISSN
2023 Impact Factor: 5.3
2023 SCImago Journal Rankings: 0.848
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMinopoli, A-
dc.contributor.authorDella Ventura, B-
dc.contributor.authorCampanile, R-
dc.contributor.authorTanner, JA-
dc.contributor.authorOffenhäusser, A-
dc.contributor.authorMayer, D-
dc.contributor.authorVelotta, R-
dc.date.accessioned2021-03-08T07:14:45Z-
dc.date.available2021-03-08T07:14:45Z-
dc.date.issued2021-
dc.identifier.citationMicrochimica Acta, 2021, v. 188 n. 3, p. article no. 88-
dc.identifier.issn0026-3672-
dc.identifier.urihttp://hdl.handle.net/10722/297142-
dc.descriptionHybrid open access-
dc.description.abstractA plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays.-
dc.languageeng-
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/chemistry/journal/604-
dc.relation.ispartofMicrochimica Acta-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectNanoplasmonics-
dc.subjectPlasmon-enhanced fluorescence-
dc.subjectPhotochemical immobilization technique-
dc.subjectAntibody-aptamer biosensor-
dc.subjectMalaria marker-
dc.titleRandomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1007/s00604-021-04746-9-
dc.identifier.pmid33594523-
dc.identifier.pmcidPMC7886758-
dc.identifier.scopuseid_2-s2.0-85101052224-
dc.identifier.hkuros321573-
dc.identifier.volume188-
dc.identifier.issue3-
dc.identifier.spagearticle no. 88-
dc.identifier.epagearticle no. 88-
dc.identifier.isiWOS:000620547300001-
dc.publisher.placeAustria-

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