File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Prokaryotic expression and purification of HA1 and HA2 polypeptides for serological analysis of the 2009 pandemic H1N1 influenza virus

TitleProkaryotic expression and purification of HA1 and HA2 polypeptides for serological analysis of the 2009 pandemic H1N1 influenza virus
Authors
KeywordsSoluble protein
Prokaryotic expression
Hemagglutinin
2009 pandemic influenza virus H1N1
Issue Date2011
Citation
Journal of Virological Methods, 2011, v. 172, n. 1-2, p. 16-21 How to Cite?
AbstractHemagglutinin (HA) is an important influenza virus surface antigen that is highly topical in influenza research. In the present study, the genes encoding the HA1 and HA2 proteins from the 2009 pandemic influenza virus H1N1 (A/California/04/2009(H1N1)) were cloned into a prokaryotic expression plasmid pCold-TF, and soluble fusion proteins containing H1N1 HA1 and HA2 were produced by transformed Escherichia coli. Western blot assays were used to examine the immunoreactivity of the recombinant proteins using polyclonal and monoclonal antibodies derived against the whole virus A/California/04/2009(H1N1). Recombinant protein immunoreactivity was also analyzed qualitatively by ELISA and hemagglutination inhibition using human serum samples. These results will aid future immunological and serological studies of the 2009 pandemic H1N1 virus HA. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/297305
ISSN
2020 Impact Factor: 2.014
2020 SCImago Journal Rankings: 0.786
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, Dabin-
dc.contributor.authorSi, Bingyin-
dc.contributor.authorLi, Cun-
dc.contributor.authorMi, Zhiqiang-
dc.contributor.authorAn, Xiaoping-
dc.contributor.authorQin, Chengfeng-
dc.contributor.authorLiu, Wei-
dc.contributor.authorTong, Yigang-
dc.date.accessioned2021-03-15T07:33:28Z-
dc.date.available2021-03-15T07:33:28Z-
dc.date.issued2011-
dc.identifier.citationJournal of Virological Methods, 2011, v. 172, n. 1-2, p. 16-21-
dc.identifier.issn0166-0934-
dc.identifier.urihttp://hdl.handle.net/10722/297305-
dc.description.abstractHemagglutinin (HA) is an important influenza virus surface antigen that is highly topical in influenza research. In the present study, the genes encoding the HA1 and HA2 proteins from the 2009 pandemic influenza virus H1N1 (A/California/04/2009(H1N1)) were cloned into a prokaryotic expression plasmid pCold-TF, and soluble fusion proteins containing H1N1 HA1 and HA2 were produced by transformed Escherichia coli. Western blot assays were used to examine the immunoreactivity of the recombinant proteins using polyclonal and monoclonal antibodies derived against the whole virus A/California/04/2009(H1N1). Recombinant protein immunoreactivity was also analyzed qualitatively by ELISA and hemagglutination inhibition using human serum samples. These results will aid future immunological and serological studies of the 2009 pandemic H1N1 virus HA. © 2010 Elsevier B.V.-
dc.languageeng-
dc.relation.ispartofJournal of Virological Methods-
dc.subjectSoluble protein-
dc.subjectProkaryotic expression-
dc.subjectHemagglutinin-
dc.subject2009 pandemic influenza virus H1N1-
dc.titleProkaryotic expression and purification of HA1 and HA2 polypeptides for serological analysis of the 2009 pandemic H1N1 influenza virus-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jviromet.2010.12.007-
dc.identifier.pmid21168444-
dc.identifier.scopuseid_2-s2.0-79451475113-
dc.identifier.volume172-
dc.identifier.issue1-2-
dc.identifier.spage16-
dc.identifier.epage21-
dc.identifier.isiWOS:000287909300003-
dc.identifier.issnl0166-0934-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats