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Article: Construction of a chimeric secretory IgA and its neutralization activity against avian influenza virus H5N1

TitleConstruction of a chimeric secretory IgA and its neutralization activity against avian influenza virus H5N1
Authors
Issue Date2014
Citation
Journal of Immunology Research, 2014, v. 2014, article no. 394127 How to Cite?
AbstractSecretory immunoglobulin A (SIgA) acts as the first line of defense against respiratory pathogens. In this assay, the variable regions of heavy chain (VH) and Light chain (VL) genes from a mouse monoclonal antibody against H5N1 were cloned and fused with human IgA constant regions. The full-length chimeric light and heavy chains were inserted into a eukaryotic expressing vector and then transfected into CHO/dhfr-cells. The chimeric monomeric IgA antibody expression was confirmed by using ELISA, SDS-PAGE, and Western blot. In order to obtain a dimeric secretory IgA, another two expressing plasmids, namely, pcDNA4/His A-IgJ and pcDNA4/His A-SC, were cotransfected into the CHO/dhfr-cells. The expression of dimeric SIgA was confirmed by using ELISA assay and native gel electrophoresis. In microneutralization assay on 96-well immunoplate, the chimeric SIgA showed neutralization activity against H5N1 virus on MDCK cells and the titer was determined to be 1: 64. On preadministrating intranasally, the chimeric SIgA could prevent mice from lethal attack by using A/Vietnam/1194/04 H5N1 with a survival rate of 80%. So we concluded that the constructed recombinant chimeric SIgA has a neutralization capability targeting avian influenza virus H5N1 infection in vitro and in vivo. © 2014 Cun Li et al.
Persistent Identifierhttp://hdl.handle.net/10722/297334
ISSN
2020 Impact Factor: 4.818
2020 SCImago Journal Rankings: 1.315
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, Cun-
dc.contributor.authorAn, Xiaoping-
dc.contributor.authorButt, Azeem Mehmood-
dc.contributor.authorZhang, Baozhong-
dc.contributor.authorZhang, Zhiyi-
dc.contributor.authorWang, Xiaona-
dc.contributor.authorHuang, Yong-
dc.contributor.authorZhang, Wenhui-
dc.contributor.authorZhang, Bo-
dc.contributor.authorMi, Zhiqiang-
dc.contributor.authorTong, Yigang-
dc.date.accessioned2021-03-15T07:33:32Z-
dc.date.available2021-03-15T07:33:32Z-
dc.date.issued2014-
dc.identifier.citationJournal of Immunology Research, 2014, v. 2014, article no. 394127-
dc.identifier.issn2314-8861-
dc.identifier.urihttp://hdl.handle.net/10722/297334-
dc.description.abstractSecretory immunoglobulin A (SIgA) acts as the first line of defense against respiratory pathogens. In this assay, the variable regions of heavy chain (VH) and Light chain (VL) genes from a mouse monoclonal antibody against H5N1 were cloned and fused with human IgA constant regions. The full-length chimeric light and heavy chains were inserted into a eukaryotic expressing vector and then transfected into CHO/dhfr-cells. The chimeric monomeric IgA antibody expression was confirmed by using ELISA, SDS-PAGE, and Western blot. In order to obtain a dimeric secretory IgA, another two expressing plasmids, namely, pcDNA4/His A-IgJ and pcDNA4/His A-SC, were cotransfected into the CHO/dhfr-cells. The expression of dimeric SIgA was confirmed by using ELISA assay and native gel electrophoresis. In microneutralization assay on 96-well immunoplate, the chimeric SIgA showed neutralization activity against H5N1 virus on MDCK cells and the titer was determined to be 1: 64. On preadministrating intranasally, the chimeric SIgA could prevent mice from lethal attack by using A/Vietnam/1194/04 H5N1 with a survival rate of 80%. So we concluded that the constructed recombinant chimeric SIgA has a neutralization capability targeting avian influenza virus H5N1 infection in vitro and in vivo. © 2014 Cun Li et al.-
dc.languageeng-
dc.relation.ispartofJournal of Immunology Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleConstruction of a chimeric secretory IgA and its neutralization activity against avian influenza virus H5N1-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2014/394127-
dc.identifier.pmid24741594-
dc.identifier.pmcidPMC3987799-
dc.identifier.scopuseid_2-s2.0-84898677793-
dc.identifier.volume2014-
dc.identifier.spagearticle no. 394127-
dc.identifier.epagearticle no. 394127-
dc.identifier.eissn2314-7156-
dc.identifier.isiWOS:000332542200001-
dc.identifier.issnl2314-7156-

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