Relevant variations and neuroprotecive effect of hydrogen sulfide in a rat glaucoma model

Abstract

Glaucoma is an irreversible and blinding neurodegenerative disease of the eye, and is characterized by progressive loss of retinal ganglion cells (RGCs). Since endogenous hydrogen sulfide (H S) was reported to be involved in neurodegeneration in the central nervous system, the authors aimed to develop a chronic ocular hypertension (COH) rat model simulating glaucoma and therein test the H S level together with the retinal protein expressions of related synthases, and further investigated the effect of exogenous H S supplement on RGC survival. COH rat model was induced by cross-linking hydrogel injection into anterior chamber, and the performance of the model was assessed by intraocular pressure (IOP) measurement, RGC counting and retinal morphological analysis. Endogenous H S level was detected along with the retinal protein expressions of H S-related synthases cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) in the COH rats. Retinal H S level and RGC survival were evaluated again after NaHS (a H S donor) treatment in the COH rats. The results showed that the COH model succeeded in simulating glaucoma features, and retinal H S level decreased significantly when the retinal protein expressions of CBS, CSE and 3-MST were downregulated generally in the COH rats. Furthermore, the decrease of retinal H S level and loss of RGCs were both improved by NaHS treatment in experimental glaucoma, without obvious variation of IOP. Our study revealed that the intracameral injection of cross-linking hydrogel worked efficiently in modeling glaucoma, and H S had protective effect on RGCs and might be involved in the pathological mechanism of glaucomatous neuropathy. 2 2 2 2 2 2 2 2 2 2