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Conference Paper: Adaptive optics two-photon microscopy for in vivo imaging of mouse retina

TitleAdaptive optics two-photon microscopy for in vivo imaging of mouse retina
Authors
KeywordsDirect wavefront sensing
Retinal imaging
Adaptive optics
Functional imaging
Two photon microscopy
Issue Date2020
Citation
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2020, v. 11248, article no. 112480L How to Cite?
AbstractNon-invasive retinal imaging has greatly facilitated the research of eye disease and neurodegenerative disorders in the central nervous system (CNS). Two-photon microscopy is a powerful tool for in vivo imaging of mouse retina because it provides intrinsic optical sectioning capability and the infrared laser is less likely to excite the photoreceptors. However, the dilated mouse eye has large optical aberrations, which must be corrected to achieve high-resolution or even diffraction-limited imaging. Here, we developed an adaptive optics (AO) two-photon microscope for in vivo imaging of retinal neurons through the eyeball of living mouse. We used the two-photon excited fluorescence signal of retina as the guide star to measure and correct the aberration of mouse eye. After AO correction, the fluorescence signal was increased by at least fivefold and the fine structures such as axons of retinal ganglion cells (RGC) were clearly resolved. To take advantage of the non-invasive high-resolution imaging, we demonstrated functional calcium imaging of RGC responding to the light stimulations.
Persistent Identifierhttp://hdl.handle.net/10722/298348
ISSN
2023 SCImago Journal Rankings: 0.226

 

DC FieldValueLanguage
dc.contributor.authorQin, Zhongya-
dc.contributor.authorHe, Sicong-
dc.contributor.authorChen, Congping-
dc.contributor.authorYang, Chao-
dc.contributor.authorYung, Jasmine-
dc.contributor.authorWu, Wanjie-
dc.contributor.authorLeung, Christopher K.-
dc.contributor.authorLiu, Kai-
dc.contributor.authorQu, Jianan Y.-
dc.date.accessioned2021-04-08T03:08:12Z-
dc.date.available2021-04-08T03:08:12Z-
dc.date.issued2020-
dc.identifier.citationProgress in Biomedical Optics and Imaging - Proceedings of SPIE, 2020, v. 11248, article no. 112480L-
dc.identifier.issn1605-7422-
dc.identifier.urihttp://hdl.handle.net/10722/298348-
dc.description.abstractNon-invasive retinal imaging has greatly facilitated the research of eye disease and neurodegenerative disorders in the central nervous system (CNS). Two-photon microscopy is a powerful tool for in vivo imaging of mouse retina because it provides intrinsic optical sectioning capability and the infrared laser is less likely to excite the photoreceptors. However, the dilated mouse eye has large optical aberrations, which must be corrected to achieve high-resolution or even diffraction-limited imaging. Here, we developed an adaptive optics (AO) two-photon microscope for in vivo imaging of retinal neurons through the eyeball of living mouse. We used the two-photon excited fluorescence signal of retina as the guide star to measure and correct the aberration of mouse eye. After AO correction, the fluorescence signal was increased by at least fivefold and the fine structures such as axons of retinal ganglion cells (RGC) were clearly resolved. To take advantage of the non-invasive high-resolution imaging, we demonstrated functional calcium imaging of RGC responding to the light stimulations.-
dc.languageeng-
dc.relation.ispartofProgress in Biomedical Optics and Imaging - Proceedings of SPIE-
dc.subjectDirect wavefront sensing-
dc.subjectRetinal imaging-
dc.subjectAdaptive optics-
dc.subjectFunctional imaging-
dc.subjectTwo photon microscopy-
dc.titleAdaptive optics two-photon microscopy for in vivo imaging of mouse retina-
dc.typeConference_Paper-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1117/12.2546972-
dc.identifier.scopuseid_2-s2.0-85082667694-
dc.identifier.volume11248-
dc.identifier.spagearticle no. 112480L-
dc.identifier.epagearticle no. 112480L-
dc.identifier.issnl1605-7422-

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