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Article: Scapula development is governed by genetic interactions of Pbx1 with its family members and with Emx2 via their cooperative control of Alx1

TitleScapula development is governed by genetic interactions of Pbx1 with its family members and with Emx2 via their cooperative control of Alx1
Authors
KeywordsScapula
Hox
Mouse
Girdle
Pbx
Emx2
Issue Date2010
Citation
Development, 2010, v. 137, n. 15, p. 2559-2569 How to Cite?
AbstractThe genetic pathways underlying shoulder blade development are largely unknown, as gene networks controlling limb morphogenesis have limited influence on scapula formation. Analysis of mouse mutants for Pbx and Emx2 genes has suggested their potential roles in girdle development. In this study, by generating compound mutant mice, we examined the genetic control of scapula development by Pbx genes and their functional relationship with Emx2. Analyses of Pbx and Pbx1;Emx2 compound mutants revealed that Pbx genes share overlapping functions in shoulder development and that Pbx1 genetically interacts with Emx2 in this process. Here, we provide a biochemical basis for Pbx1;Emx2 genetic interaction by showing that Pbx1 and Emx2 can bind specific DNA sequences as heterodimers. Moreover, the expression of genes crucial for scapula development is altered in these mutants, indicating that Pbx genes act upstream of essential pathways for scapula formation. In particular, expression of Alx1, an effector of scapula blade patterning, is absent in all compound mutants. We demonstrate that Pbx1 and Emx2 bind in vivo to a conserved sequence upstream of Alx1 and cooperatively activate its transcription via this potential regulatory element. Our results establish an essential role for Pbx1 in genetic interactions with its family members and with Emx2 and delineate novel regulatory networks in shoulder girdle development.
Persistent Identifierhttp://hdl.handle.net/10722/298502
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 1.852
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCapellini, Terence D.-
dc.contributor.authorVaccari, Giulia-
dc.contributor.authorFerretti, Elisabetta-
dc.contributor.authorFantini, Sebastian-
dc.contributor.authorHe, Mu-
dc.contributor.authorPellegrini, Massimo-
dc.contributor.authorQuintana, Laura-
dc.contributor.authorDi Giacomo, Giuseppina-
dc.contributor.authorSharpe, James-
dc.contributor.authorSelleri, Licia-
dc.contributor.authorZappavigna, Vincenzo-
dc.date.accessioned2021-04-08T03:08:38Z-
dc.date.available2021-04-08T03:08:38Z-
dc.date.issued2010-
dc.identifier.citationDevelopment, 2010, v. 137, n. 15, p. 2559-2569-
dc.identifier.issn0950-1991-
dc.identifier.urihttp://hdl.handle.net/10722/298502-
dc.description.abstractThe genetic pathways underlying shoulder blade development are largely unknown, as gene networks controlling limb morphogenesis have limited influence on scapula formation. Analysis of mouse mutants for Pbx and Emx2 genes has suggested their potential roles in girdle development. In this study, by generating compound mutant mice, we examined the genetic control of scapula development by Pbx genes and their functional relationship with Emx2. Analyses of Pbx and Pbx1;Emx2 compound mutants revealed that Pbx genes share overlapping functions in shoulder development and that Pbx1 genetically interacts with Emx2 in this process. Here, we provide a biochemical basis for Pbx1;Emx2 genetic interaction by showing that Pbx1 and Emx2 can bind specific DNA sequences as heterodimers. Moreover, the expression of genes crucial for scapula development is altered in these mutants, indicating that Pbx genes act upstream of essential pathways for scapula formation. In particular, expression of Alx1, an effector of scapula blade patterning, is absent in all compound mutants. We demonstrate that Pbx1 and Emx2 bind in vivo to a conserved sequence upstream of Alx1 and cooperatively activate its transcription via this potential regulatory element. Our results establish an essential role for Pbx1 in genetic interactions with its family members and with Emx2 and delineate novel regulatory networks in shoulder girdle development.-
dc.languageeng-
dc.relation.ispartofDevelopment-
dc.subjectScapula-
dc.subjectHox-
dc.subjectMouse-
dc.subjectGirdle-
dc.subjectPbx-
dc.subjectEmx2-
dc.titleScapula development is governed by genetic interactions of Pbx1 with its family members and with Emx2 via their cooperative control of Alx1-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1242/dev.048819-
dc.identifier.pmid20627960-
dc.identifier.pmcidPMC2927673-
dc.identifier.scopuseid_2-s2.0-77955757308-
dc.identifier.volume137-
dc.identifier.issue15-
dc.identifier.spage2559-
dc.identifier.epage2569-
dc.identifier.eissn1477-9129-
dc.identifier.isiWOS:000279828300013-
dc.identifier.issnl0950-1991-

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