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Article: The overexpression of rice ACYL-COA-BINDING PROTEIN4 improves salinity tolerance in transgenic rice

TitleThe overexpression of rice ACYL-COA-BINDING PROTEIN4 improves salinity tolerance in transgenic rice
Authors
KeywordsAcyl-CoA synthase
Arabidopsis thaliana
ITC
Lipid profiling
Oryza sativa
Issue Date2021
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/envexpbot
Citation
Environmental and Experimental Botany, 2021, v. 183, p. article no. 104349 How to Cite?
AbstractIncreased salinity, as consequence of the ongoing climate change, has a significant impact on crop production worldwide. The overexpression of rice ACYL-COA-BINDING PROTEIN4 (OsACBP4) protects transgenic rice and Arabidopsis from salinity stress, while OsACBP4-RNAi lines were more susceptible than the vector control (VC) and wild type (WT). The overexpression of AtACBP2, the Arabidopsis OsACBP4 homologue, also conferred salinity protection in transgenic rice and Arabidopsis. When OsACBP4 expression was investigated using ß-glucuronidase (GUS) reporter fusion, OsACBP4pro::GUS expression was induced after salt treatment of transgenic rice seedlings. Electrophoretic mobility shift assays verified that four salinity-responsive elements in the OsACBP4 5’-flanking region interacts with nuclear protein from salt-treated rice. RNA-sequencing and qRT-PCR on salt-treated rice OsACBP4-overexpressors (OEs), revealed an upregulation of all five genes encoding acyl-CoA synthase, essential in fatty acid elongation, over the controls. Furthermore, GC–MS analysis showed differences in fatty acid content between the leaves of the OsACBP4-OEs and the controls. Taken together, OsACBP4 may play a role in salinity responses by regulating lipid metabolism through binding acyl-CoA esters in vegetative tissues as recombinant OsACBP4 was subsequently confirmed to bind long-chain acyl-CoA esters in isothermal titration calorimetry assays.
Persistent Identifierhttp://hdl.handle.net/10722/299090
ISSN
2020 Impact Factor: 5.545
2015 SCImago Journal Rankings: 1.340
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGUO, ZH-
dc.contributor.authorPOGANCEV, G-
dc.contributor.authorMeng, W-
dc.contributor.authorDu, ZY-
dc.contributor.authorLiao, P-
dc.contributor.authorZhang, R-
dc.contributor.authorChye, ML-
dc.date.accessioned2021-04-28T02:26:03Z-
dc.date.available2021-04-28T02:26:03Z-
dc.date.issued2021-
dc.identifier.citationEnvironmental and Experimental Botany, 2021, v. 183, p. article no. 104349-
dc.identifier.issn0098-8472-
dc.identifier.urihttp://hdl.handle.net/10722/299090-
dc.description.abstractIncreased salinity, as consequence of the ongoing climate change, has a significant impact on crop production worldwide. The overexpression of rice ACYL-COA-BINDING PROTEIN4 (OsACBP4) protects transgenic rice and Arabidopsis from salinity stress, while OsACBP4-RNAi lines were more susceptible than the vector control (VC) and wild type (WT). The overexpression of AtACBP2, the Arabidopsis OsACBP4 homologue, also conferred salinity protection in transgenic rice and Arabidopsis. When OsACBP4 expression was investigated using ß-glucuronidase (GUS) reporter fusion, OsACBP4pro::GUS expression was induced after salt treatment of transgenic rice seedlings. Electrophoretic mobility shift assays verified that four salinity-responsive elements in the OsACBP4 5’-flanking region interacts with nuclear protein from salt-treated rice. RNA-sequencing and qRT-PCR on salt-treated rice OsACBP4-overexpressors (OEs), revealed an upregulation of all five genes encoding acyl-CoA synthase, essential in fatty acid elongation, over the controls. Furthermore, GC–MS analysis showed differences in fatty acid content between the leaves of the OsACBP4-OEs and the controls. Taken together, OsACBP4 may play a role in salinity responses by regulating lipid metabolism through binding acyl-CoA esters in vegetative tissues as recombinant OsACBP4 was subsequently confirmed to bind long-chain acyl-CoA esters in isothermal titration calorimetry assays.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/envexpbot-
dc.relation.ispartofEnvironmental and Experimental Botany-
dc.subjectAcyl-CoA synthase-
dc.subjectArabidopsis thaliana-
dc.subjectITC-
dc.subjectLipid profiling-
dc.subjectOryza sativa-
dc.titleThe overexpression of rice ACYL-COA-BINDING PROTEIN4 improves salinity tolerance in transgenic rice-
dc.typeArticle-
dc.identifier.emailChye, ML: mlchye@hku.hk-
dc.identifier.authorityChye, ML=rp00687-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.envexpbot.2020.104349-
dc.identifier.scopuseid_2-s2.0-85098885260-
dc.identifier.hkuros322313-
dc.identifier.volume183-
dc.identifier.spagearticle no. 104349-
dc.identifier.epagearticle no. 104349-
dc.identifier.isiWOS:000608802700015-
dc.publisher.placeNetherlands-

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