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Article: Collagen IV differentially regulates planarian stem cell potency and lineage progression

TitleCollagen IV differentially regulates planarian stem cell potency and lineage progression
Authors
Keywordsplanarian
extracellular matrix
stem cell niche
pluripotency
regeneration
Issue Date2021
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2021, v. 118, p. article no. e2021251118 How to Cite?
AbstractThe extracellular matrix (ECM) provides a precise physical and molecular environment for cell maintenance, self-renewal, and differentiation in the stem cell niche. However, the nature and organization of the ECM niche is not well understood. The adult freshwater planarian Schmidtea mediterranea maintains a large population of multipotent stem cells (neoblasts), presenting an ideal model to study the role of the ECM niche in stem cell regulation. Here we tested the function of 165 planarian homologs of ECM and ECM-related genes in neoblast regulation. We identified the collagen gene family as one with differential effects in promoting or suppressing proliferation of neoblasts. col4-1, encoding a type IV collagen α-chain, had the strongest effect. RNA interference (RNAi) of col4-1 impaired tissue maintenance and regeneration, causing tissue regression. Finally, we provide evidence for an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 (NRG-7), which constitutes a mechanism to regulate the balance of symmetric and asymmetric division of neoblasts via the NRG-7/EGFR pathway.
DescriptionHybrid open access
Persistent Identifierhttp://hdl.handle.net/10722/299295
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, A-
dc.contributor.authorMA, S-
dc.contributor.authorPearson, BJ-
dc.contributor.authorChan, D-
dc.date.accessioned2021-05-10T06:59:47Z-
dc.date.available2021-05-10T06:59:47Z-
dc.date.issued2021-
dc.identifier.citationProceedings of the National Academy of Sciences, 2021, v. 118, p. article no. e2021251118-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/299295-
dc.descriptionHybrid open access-
dc.description.abstractThe extracellular matrix (ECM) provides a precise physical and molecular environment for cell maintenance, self-renewal, and differentiation in the stem cell niche. However, the nature and organization of the ECM niche is not well understood. The adult freshwater planarian Schmidtea mediterranea maintains a large population of multipotent stem cells (neoblasts), presenting an ideal model to study the role of the ECM niche in stem cell regulation. Here we tested the function of 165 planarian homologs of ECM and ECM-related genes in neoblast regulation. We identified the collagen gene family as one with differential effects in promoting or suppressing proliferation of neoblasts. col4-1, encoding a type IV collagen α-chain, had the strongest effect. RNA interference (RNAi) of col4-1 impaired tissue maintenance and regeneration, causing tissue regression. Finally, we provide evidence for an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 (NRG-7), which constitutes a mechanism to regulate the balance of symmetric and asymmetric division of neoblasts via the NRG-7/EGFR pathway.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsProceedings of the National Academy of Sciences. Copyright © National Academy of Sciences.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectplanarian-
dc.subjectextracellular matrix-
dc.subjectstem cell niche-
dc.subjectpluripotency-
dc.subjectregeneration-
dc.titleCollagen IV differentially regulates planarian stem cell potency and lineage progression-
dc.typeArticle-
dc.identifier.emailChan, D: chand@hku.hk-
dc.identifier.authorityChan, D=rp00540-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.2021251118-
dc.identifier.pmid33859045-
dc.identifier.pmcidPMC8072372-
dc.identifier.scopuseid_2-s2.0-85104386259-
dc.identifier.hkuros322423-
dc.identifier.volume118-
dc.identifier.spagearticle no. e2021251118-
dc.identifier.epagearticle no. e2021251118-
dc.identifier.isiWOS:000664390900011-
dc.publisher.placeUnited States-

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