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Book Chapter: Unequivocal single-molecule force spectroscopy of intrinsically disordered proteins

TitleUnequivocal single-molecule force spectroscopy of intrinsically disordered proteins
Authors
KeywordsAtomic force spectroscopy
Protein nanomechanics
Single-molecule force spectroscopy
Intrinsically disordered proteins
Conformational plasticity
Issue Date2012
PublisherSpringer.
Citation
Unequivocal single-molecule force spectroscopy of intrinsically disordered proteins. In Uversky, VN, Dunker, AK (Eds.), Intrinsically Disordered Protein Analysis: Volume 2, Methods and Experimental Tools, p. 71-87. New York, NY: Springer, 2012 How to Cite?
AbstractIntrinsically disordered proteins (IDPs) are predicted to represent about one third of the eukaryotic proteome. The dynamic ensemble of conformations of this steadily growing class of proteins has remained hardly accessible for bulk biophysical techniques. However, single-molecule techniques provide a useful means of studying these proteins. Atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is one of such techniques, which has certain peculiarities that make it an important methodology to analyze the biophysical properties of IDPs. However, several drawbacks inherent to this technique can complicate such analysis. We have developed a protein engineering strategy to overcome these drawbacks such that an unambiguous mechanical analysis of proteins, including IDPs, can be readily performed. Using this approach, we have recently characterized the rich conformational polymorphism of several IDPs. Here, we describe a simple protocol to perform the nanomechanical analysis of IDPs using this new strategy, a procedure that in principle can also be followed for the nanomechanical analysis of any protein. © 2012 Springer Science+Business Media New York.
Persistent Identifierhttp://hdl.handle.net/10722/299495
ISBN
ISSN
2023 SCImago Journal Rankings: 0.399
Series/Report no.Methods in Molecular Biology ; 896

 

DC FieldValueLanguage
dc.contributor.authorOroz, Javier-
dc.contributor.authorHervás, Rubén-
dc.contributor.authorValbuena, Alejandro-
dc.contributor.authorCarrión-Vázquez, Mariano-
dc.date.accessioned2021-05-21T03:34:32Z-
dc.date.available2021-05-21T03:34:32Z-
dc.date.issued2012-
dc.identifier.citationUnequivocal single-molecule force spectroscopy of intrinsically disordered proteins. In Uversky, VN, Dunker, AK (Eds.), Intrinsically Disordered Protein Analysis: Volume 2, Methods and Experimental Tools, p. 71-87. New York, NY: Springer, 2012-
dc.identifier.isbn9781461437031-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/299495-
dc.description.abstractIntrinsically disordered proteins (IDPs) are predicted to represent about one third of the eukaryotic proteome. The dynamic ensemble of conformations of this steadily growing class of proteins has remained hardly accessible for bulk biophysical techniques. However, single-molecule techniques provide a useful means of studying these proteins. Atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is one of such techniques, which has certain peculiarities that make it an important methodology to analyze the biophysical properties of IDPs. However, several drawbacks inherent to this technique can complicate such analysis. We have developed a protein engineering strategy to overcome these drawbacks such that an unambiguous mechanical analysis of proteins, including IDPs, can be readily performed. Using this approach, we have recently characterized the rich conformational polymorphism of several IDPs. Here, we describe a simple protocol to perform the nanomechanical analysis of IDPs using this new strategy, a procedure that in principle can also be followed for the nanomechanical analysis of any protein. © 2012 Springer Science+Business Media New York.-
dc.languageeng-
dc.publisherSpringer.-
dc.relation.ispartofIntrinsically Disordered Protein Analysis: Volume 2, Methods and Experimental Tools-
dc.relation.ispartofseriesMethods in Molecular Biology ; 896-
dc.subjectAtomic force spectroscopy-
dc.subjectProtein nanomechanics-
dc.subjectSingle-molecule force spectroscopy-
dc.subjectIntrinsically disordered proteins-
dc.subjectConformational plasticity-
dc.titleUnequivocal single-molecule force spectroscopy of intrinsically disordered proteins-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4614-3704-8_5-
dc.identifier.pmid22821518-
dc.identifier.scopuseid_2-s2.0-84865340909-
dc.identifier.spage71-
dc.identifier.epage87-
dc.publisher.placeNew York, NY-

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