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Article: Differences between common endothelial cell models (primary human aortic endothelial cells and EA.hy926 cells) revealed through transcriptomics, bioinformatics, and functional analysis
Title | Differences between common endothelial cell models (primary human aortic endothelial cells and EA.hy926 cells) revealed through transcriptomics, bioinformatics, and functional analysis |
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Authors | |
Keywords | Endothelial cells Bioinformatics analysis Rap1 signaling pathway Ras signaling pathway HDL cellular association |
Issue Date | 2021 |
Publisher | Elsevier: Open Access Journals. The Journal's web site is located at https://www.journals.elsevier.com/current-research-in-biotechnology |
Citation | Current Research in Biotechnology, 2021, v. 3, p. 135-145 How to Cite? |
Abstract | Endothelial cells (ECs) are involved in various physiological process. Both primary human ECs and immortal endothelial cells are used in various studies. Available genomic or transcriptomic information for difference in ECs is deficient. Therefore, in this study we aim to reveal the difference between primary human aortic ECs (HAECs) and immortal EA.hy926 cells. We identified 529 differentially expressed genes (DEGs) between HAECs and EA.hy926 cells. Gene Ontology (GO), KEGG Pathway and GSEA enrichment analysis suggest that DEGs highly expressed in HAECs are distributed in Rap1 signaling pathway and Ras signaling pathway, which are contributing to the endothelial barrier function and endocytosis, among other functions. We also established long non-coding (lncRNA)-miRNA-mRNA ceRNA network, and further set up protein–protein interaction (PPI) network. High-density lipoprotein (HDL) cellular association experiments were verified that HAECs have stronger response to HDL cellular binding and endocytosis compared to EA.hy926 cells. This study identified DEGs between HAECs and EA.hy926 cells, and found enrichment of the Ras signaling pathway and Rap1 signaling pathway in HAECs, established ceRNA network and suggested that HAECs may have a stronger response to endothelial binding and endocytosis compared to EA.hy926 cells. This work provides a genomic basis to choose suitable EC model to reach respective research goals. |
Persistent Identifier | http://hdl.handle.net/10722/300246 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 0.702 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, D | - |
dc.contributor.author | Chen, Z | - |
dc.contributor.author | Yeung, AWK | - |
dc.contributor.author | Atanasov, AG | - |
dc.date.accessioned | 2021-06-04T08:40:14Z | - |
dc.date.available | 2021-06-04T08:40:14Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Current Research in Biotechnology, 2021, v. 3, p. 135-145 | - |
dc.identifier.issn | 2590-2628 | - |
dc.identifier.uri | http://hdl.handle.net/10722/300246 | - |
dc.description.abstract | Endothelial cells (ECs) are involved in various physiological process. Both primary human ECs and immortal endothelial cells are used in various studies. Available genomic or transcriptomic information for difference in ECs is deficient. Therefore, in this study we aim to reveal the difference between primary human aortic ECs (HAECs) and immortal EA.hy926 cells. We identified 529 differentially expressed genes (DEGs) between HAECs and EA.hy926 cells. Gene Ontology (GO), KEGG Pathway and GSEA enrichment analysis suggest that DEGs highly expressed in HAECs are distributed in Rap1 signaling pathway and Ras signaling pathway, which are contributing to the endothelial barrier function and endocytosis, among other functions. We also established long non-coding (lncRNA)-miRNA-mRNA ceRNA network, and further set up protein–protein interaction (PPI) network. High-density lipoprotein (HDL) cellular association experiments were verified that HAECs have stronger response to HDL cellular binding and endocytosis compared to EA.hy926 cells. This study identified DEGs between HAECs and EA.hy926 cells, and found enrichment of the Ras signaling pathway and Rap1 signaling pathway in HAECs, established ceRNA network and suggested that HAECs may have a stronger response to endothelial binding and endocytosis compared to EA.hy926 cells. This work provides a genomic basis to choose suitable EC model to reach respective research goals. | - |
dc.language | eng | - |
dc.publisher | Elsevier: Open Access Journals. The Journal's web site is located at https://www.journals.elsevier.com/current-research-in-biotechnology | - |
dc.relation.ispartof | Current Research in Biotechnology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Endothelial cells | - |
dc.subject | Bioinformatics analysis | - |
dc.subject | Rap1 signaling pathway | - |
dc.subject | Ras signaling pathway | - |
dc.subject | HDL cellular association | - |
dc.title | Differences between common endothelial cell models (primary human aortic endothelial cells and EA.hy926 cells) revealed through transcriptomics, bioinformatics, and functional analysis | - |
dc.type | Article | - |
dc.identifier.email | Yeung, AWK: ndyeung@hku.hk | - |
dc.identifier.authority | Yeung, AWK=rp02143 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.crbiot.2021.05.001 | - |
dc.identifier.scopus | eid_2-s2.0-85107071123 | - |
dc.identifier.hkuros | 322707 | - |
dc.identifier.volume | 3 | - |
dc.identifier.spage | 135 | - |
dc.identifier.epage | 145 | - |
dc.identifier.isi | WOS:000739728600015 | - |
dc.publisher.place | Netherlands | - |