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Article: Rapid Broad Spectrum Detection of Carbapenemases with a Dual Fluorogenic-Colorimetric Probe
Title | Rapid Broad Spectrum Detection of Carbapenemases with a Dual Fluorogenic-Colorimetric Probe |
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Authors | |
Issue Date | 2021 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html |
Citation | Journal of the American Chemical Society, 2021, v. 143 n. 18, p. 6886-6894 How to Cite? |
Abstract | Carbapenems stand as one of the last-resort antibiotics; however, their efficacy is threatened by the rising number and rapid spread of carbapenemases. Effective antimicrobial stewardship thus calls for rapid tests for these enzymes to aid appropriate prescription and infection control. Herein, we report the first effective pan-carbapenemase reporter CARBA-H with a broad scope covering all three Ambler classes. Using a chemical biology approach, we demonstrated that the absence of the 1β-substituent in the carbapenem core is key to pan-carbapenemase recognition, which led to our rational design and probe development. CARBA-H provides a dual colorimetric-fluorogenic response upon carbapenemase-mediated hydrolysis. A clear visual readout can be obtained within 15 min when tested against a panel of carbapenemase-producing Enterobacteriaceae (CPE) clinical isolates that notably includes OXA-48 and OXA-181-producing strains. Furthermore, CARBA-H can be applied to the detection of carbapemenase activity in CPE-spiked urine samples. |
Description | Hybrid open access |
Persistent Identifier | http://hdl.handle.net/10722/300299 |
ISSN | 2023 Impact Factor: 14.4 2023 SCImago Journal Rankings: 5.489 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ma, CW | - |
dc.contributor.author | Ng, KKH | - |
dc.contributor.author | Yam, BHC | - |
dc.contributor.author | Ho, PL | - |
dc.contributor.author | Kao, RYT | - |
dc.contributor.author | Yang, D | - |
dc.date.accessioned | 2021-06-04T08:40:59Z | - |
dc.date.available | 2021-06-04T08:40:59Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of the American Chemical Society, 2021, v. 143 n. 18, p. 6886-6894 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/10722/300299 | - |
dc.description | Hybrid open access | - |
dc.description.abstract | Carbapenems stand as one of the last-resort antibiotics; however, their efficacy is threatened by the rising number and rapid spread of carbapenemases. Effective antimicrobial stewardship thus calls for rapid tests for these enzymes to aid appropriate prescription and infection control. Herein, we report the first effective pan-carbapenemase reporter CARBA-H with a broad scope covering all three Ambler classes. Using a chemical biology approach, we demonstrated that the absence of the 1β-substituent in the carbapenem core is key to pan-carbapenemase recognition, which led to our rational design and probe development. CARBA-H provides a dual colorimetric-fluorogenic response upon carbapenemase-mediated hydrolysis. A clear visual readout can be obtained within 15 min when tested against a panel of carbapenemase-producing Enterobacteriaceae (CPE) clinical isolates that notably includes OXA-48 and OXA-181-producing strains. Furthermore, CARBA-H can be applied to the detection of carbapemenase activity in CPE-spiked urine samples. | - |
dc.language | eng | - |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html | - |
dc.relation.ispartof | Journal of the American Chemical Society | - |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Rapid Broad Spectrum Detection of Carbapenemases with a Dual Fluorogenic-Colorimetric Probe | - |
dc.type | Article | - |
dc.identifier.email | Ma, CW: mcwhk@connect.hku.hk | - |
dc.identifier.email | Ng, KKH: kkhn3@hku.hk | - |
dc.identifier.email | Yam, BHC: yamhcb@hku.hk | - |
dc.identifier.email | Ho, PL: plho@hku.hk | - |
dc.identifier.email | Kao, RYT: rytkao@hkucc.hku.hk | - |
dc.identifier.email | Yang, D: yangdan@hku.hk | - |
dc.identifier.authority | Ng, KKH=rp02424 | - |
dc.identifier.authority | Ho, PL=rp00406 | - |
dc.identifier.authority | Kao, RYT=rp00481 | - |
dc.identifier.authority | Yang, D=rp00825 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1021/jacs.1c00462 | - |
dc.identifier.scopus | eid_2-s2.0-85106472075 | - |
dc.identifier.hkuros | 322721 | - |
dc.identifier.volume | 143 | - |
dc.identifier.issue | 18 | - |
dc.identifier.spage | 6886 | - |
dc.identifier.epage | 6894 | - |
dc.identifier.isi | WOS:000651748000019 | - |
dc.publisher.place | United States | - |