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Article: ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8

TitleERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
Authors
Issue Date2020
PublisherOxford University Press: Policy C - Creative Commons Attribution and Creative Commons Attribution Non-Commercial. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2020, v. 48 n. 19, p. 11097-11112 How to Cite?
AbstractThe microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate ‘cluster assistance’ in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway.
Persistent Identifierhttp://hdl.handle.net/10722/300537
ISSN
2023 Impact Factor: 16.6
2023 SCImago Journal Rankings: 7.048
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwon, SC-
dc.contributor.authorJang, H-
dc.contributor.authorShen, S-
dc.contributor.authorBaek, SC-
dc.contributor.authorKim, K-
dc.contributor.authorYang, J-
dc.contributor.authorKim, J-
dc.contributor.authorKim, JS-
dc.contributor.authorWang, S-
dc.contributor.authorShi, Y-
dc.contributor.authorLi, F-
dc.contributor.authorKim, VN-
dc.date.accessioned2021-06-18T14:53:23Z-
dc.date.available2021-06-18T14:53:23Z-
dc.date.issued2020-
dc.identifier.citationNucleic Acids Research, 2020, v. 48 n. 19, p. 11097-11112-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10722/300537-
dc.description.abstractThe microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate ‘cluster assistance’ in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway.-
dc.languageeng-
dc.publisherOxford University Press: Policy C - Creative Commons Attribution and Creative Commons Attribution Non-Commercial. The Journal's web site is located at http://nar.oxfordjournals.org/-
dc.relation.ispartofNucleic Acids Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8-
dc.typeArticle-
dc.identifier.emailKwon, SC: chul@hku.hk-
dc.identifier.authorityKwon, SC=rp02691-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/nar/gkaa827-
dc.identifier.pmid33035348-
dc.identifier.pmcidPMC7641749-
dc.identifier.scopuseid_2-s2.0-85095799384-
dc.identifier.hkuros322798-
dc.identifier.volume48-
dc.identifier.issue19-
dc.identifier.spage11097-
dc.identifier.epage11112-
dc.identifier.isiWOS:000606018400040-
dc.publisher.placeUnited Kingdom-

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