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Article: Differential RNA Editing and Intron Splicing in Soybean Mitochondria during Nodulation

TitleDifferential RNA Editing and Intron Splicing in Soybean Mitochondria during Nodulation
Authors
Keywordscomplex I
intron splicing
maturase
NDH
RNA editing
Issue Date2020
PublisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms
Citation
International Journal of Molecular Sciences, 2020, v. 21 n. 24, p. article no. 9378 How to Cite?
AbstractNitrogen fixation in soybean consumes a tremendous amount of energy, leading to substantial differences in energy metabolism and mitochondrial activities between nodules and uninoculated roots. While C-to-U RNA editing and intron splicing of mitochondrial transcripts are common in plant species, their roles in relation to nodule functions are still elusive. In this study, we performed RNA-seq to compare transcript profiles and RNA editing of mitochondrial genes in soybean nodules and roots. A total of 631 RNA editing sites were identified on mitochondrial transcripts, with 12% or 74 sites differentially edited among the transcripts isolated from nodules, stripped roots, and uninoculated roots. Eight out of these 74 differentially edited sites are located on the matR transcript, of which the degrees of RNA editing were the highest in the nodule sample. The degree of mitochondrial intron splicing was also examined. The splicing efficiencies of several introns in nodules and stripped roots were higher than in uninoculated roots. These include nad1 introns 2/3/4, nad4 intron 3, nad5 introns 2/3, cox2 intron 1, and ccmFc intron 1. A greater splicing efficiency of nad4 intron 1, a higher NAD4 protein abundance, and a reduction in supercomplex I + III2 were also observed in nodules, although the causal relationship between these observations requires further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/300889
ISSN
2023 Impact Factor: 4.9
2023 SCImago Journal Rankings: 1.179
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Y-
dc.contributor.authorXie, M-
dc.contributor.authorXu, Z-
dc.contributor.authorChan, KC-
dc.contributor.authorZhong, JY-
dc.contributor.authorFan, K-
dc.contributor.authorWong-Bajracharya, J-
dc.contributor.authorLam, HM-
dc.contributor.authorLim, BL-
dc.date.accessioned2021-07-06T03:11:37Z-
dc.date.available2021-07-06T03:11:37Z-
dc.date.issued2020-
dc.identifier.citationInternational Journal of Molecular Sciences, 2020, v. 21 n. 24, p. article no. 9378-
dc.identifier.issn1661-6596-
dc.identifier.urihttp://hdl.handle.net/10722/300889-
dc.description.abstractNitrogen fixation in soybean consumes a tremendous amount of energy, leading to substantial differences in energy metabolism and mitochondrial activities between nodules and uninoculated roots. While C-to-U RNA editing and intron splicing of mitochondrial transcripts are common in plant species, their roles in relation to nodule functions are still elusive. In this study, we performed RNA-seq to compare transcript profiles and RNA editing of mitochondrial genes in soybean nodules and roots. A total of 631 RNA editing sites were identified on mitochondrial transcripts, with 12% or 74 sites differentially edited among the transcripts isolated from nodules, stripped roots, and uninoculated roots. Eight out of these 74 differentially edited sites are located on the matR transcript, of which the degrees of RNA editing were the highest in the nodule sample. The degree of mitochondrial intron splicing was also examined. The splicing efficiencies of several introns in nodules and stripped roots were higher than in uninoculated roots. These include nad1 introns 2/3/4, nad4 intron 3, nad5 introns 2/3, cox2 intron 1, and ccmFc intron 1. A greater splicing efficiency of nad4 intron 1, a higher NAD4 protein abundance, and a reduction in supercomplex I + III2 were also observed in nodules, although the causal relationship between these observations requires further investigation.-
dc.languageeng-
dc.publisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms-
dc.relation.ispartofInternational Journal of Molecular Sciences-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcomplex I-
dc.subjectintron splicing-
dc.subjectmaturase-
dc.subjectNDH-
dc.subjectRNA editing-
dc.titleDifferential RNA Editing and Intron Splicing in Soybean Mitochondria during Nodulation-
dc.typeArticle-
dc.identifier.emailChan, KC: u3532378@connect.hku.hk-
dc.identifier.emailLim, BL: bllim@hku.hk-
dc.identifier.authorityLim, BL=rp00744-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/ijms21249378-
dc.identifier.pmid33317061-
dc.identifier.pmcidPMC7764374-
dc.identifier.scopuseid_2-s2.0-85097528530-
dc.identifier.hkuros323279-
dc.identifier.volume21-
dc.identifier.issue24-
dc.identifier.spagearticle no. 9378-
dc.identifier.epagearticle no. 9378-
dc.identifier.isiWOS:000602854400001-
dc.publisher.placeSwitzerland-

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