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Article: Role of gut microbiota in travel-related acquisition of extended spectrum β-lactamase-producing Enterobacteriaceae

TitleRole of gut microbiota in travel-related acquisition of extended spectrum β-lactamase-producing Enterobacteriaceae
Authors
KeywordsGut microbiota
travel
ESBL-Enterobacteriaceae
antimicrobial resistance
Issue Date2021
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1195-1982
Citation
Journal of Travel Medicine, 2021, v. 28 n. 3, p. article no. taab022 How to Cite?
AbstractBackground International travel could facilitate the spread of antimicrobial-resistant bacteria including extended spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E). Previous studies, which attempted to understand the role of gut microbiota in the acquisition of antimicrobial resistant bacteria during international travels, are limited to western travellers. Methods We established a prospective cohort of 90 Hong Kong travellers to investigate gut microbiota determinants and associated risk factors for the acquisition of ESBL-E. Baseline characteristics and travel-associated risk factors were gathered through questionnaires. Faecal samples were collected in 3-4 days before and after travel. Antimicrobial susceptibility of ESBL-E isolates was tested, and gut microbiota were profiled by 16S rDNA amplicon sequencing. Non-parametric tests were used to detect potential associations, and logistic regression models were used to quantify the associations. Random forest models were constructed to identify microbial predictors for ESBL-E acquisition. Results In total, 49 (54.4%) participants were tested negative for ESBL-E colonization before travel and were followed up after travel. A total of 60 ESBL-E isolates were cultured from 20 (40.8%) participants. Having low Actinobacteria richness and low abundance of short-chain fatty acid-producing bacteria in the gut microbiota before travel increased the risk of acquiring ESBL-E and the risk can be further exacerbated by eating raw seafood during travel. Besides, post-travel ESBL-E positive participants had increased abundances of several opportunistic pathogens such as Staphylococcus, Enterococcus, Escherichia/Shigella and Klebsiella. The random forest model integrating pre-travel microbiota and the identified travel-related risk factor could predict ESBL-E acquisition with an area under the curve of 75.4% (95% confidence interval: 57.9–93.0%). Conclusions In this study, we identified both travel-related risk factors and microbiota predictors for the risk of ESBL-E acquisition. Our results provide foundational knowledge for future developments of microbiota-based interventions to prevent ESBL-E acquisition during international travels.
Persistent Identifierhttp://hdl.handle.net/10722/301152
ISSN
2020 Impact Factor: 8.49
2015 SCImago Journal Rankings: 0.946

 

DC FieldValueLanguage
dc.contributor.authorPENG, Y-
dc.contributor.authorLIANG, S-
dc.contributor.authorPoonsuk, K-
dc.contributor.authorOn, H-
dc.contributor.authorLi, SW-
dc.contributor.authorMaurin, MMP-
dc.contributor.authorChan, CH-
dc.contributor.authorCHAN, CL-
dc.contributor.authorSin, ZY-
dc.contributor.authorTun, HM-
dc.date.accessioned2021-07-27T08:06:54Z-
dc.date.available2021-07-27T08:06:54Z-
dc.date.issued2021-
dc.identifier.citationJournal of Travel Medicine, 2021, v. 28 n. 3, p. article no. taab022-
dc.identifier.issn1195-1982-
dc.identifier.urihttp://hdl.handle.net/10722/301152-
dc.description.abstractBackground International travel could facilitate the spread of antimicrobial-resistant bacteria including extended spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E). Previous studies, which attempted to understand the role of gut microbiota in the acquisition of antimicrobial resistant bacteria during international travels, are limited to western travellers. Methods We established a prospective cohort of 90 Hong Kong travellers to investigate gut microbiota determinants and associated risk factors for the acquisition of ESBL-E. Baseline characteristics and travel-associated risk factors were gathered through questionnaires. Faecal samples were collected in 3-4 days before and after travel. Antimicrobial susceptibility of ESBL-E isolates was tested, and gut microbiota were profiled by 16S rDNA amplicon sequencing. Non-parametric tests were used to detect potential associations, and logistic regression models were used to quantify the associations. Random forest models were constructed to identify microbial predictors for ESBL-E acquisition. Results In total, 49 (54.4%) participants were tested negative for ESBL-E colonization before travel and were followed up after travel. A total of 60 ESBL-E isolates were cultured from 20 (40.8%) participants. Having low Actinobacteria richness and low abundance of short-chain fatty acid-producing bacteria in the gut microbiota before travel increased the risk of acquiring ESBL-E and the risk can be further exacerbated by eating raw seafood during travel. Besides, post-travel ESBL-E positive participants had increased abundances of several opportunistic pathogens such as Staphylococcus, Enterococcus, Escherichia/Shigella and Klebsiella. The random forest model integrating pre-travel microbiota and the identified travel-related risk factor could predict ESBL-E acquisition with an area under the curve of 75.4% (95% confidence interval: 57.9–93.0%). Conclusions In this study, we identified both travel-related risk factors and microbiota predictors for the risk of ESBL-E acquisition. Our results provide foundational knowledge for future developments of microbiota-based interventions to prevent ESBL-E acquisition during international travels.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1195-1982-
dc.relation.ispartofJournal of Travel Medicine-
dc.rightsSubmitted (preprint) Version This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Accepted (peer-reviewed) Version This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectGut microbiota-
dc.subjecttravel-
dc.subjectESBL-Enterobacteriaceae-
dc.subjectantimicrobial resistance-
dc.titleRole of gut microbiota in travel-related acquisition of extended spectrum β-lactamase-producing Enterobacteriaceae-
dc.typeArticle-
dc.identifier.emailOn, H: hildaon@hku.hk-
dc.identifier.emailTun, HM: heinmtun@hku.hk-
dc.identifier.authorityTun, HM=rp02389-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jtm/taab022-
dc.identifier.pmid33615366-
dc.identifier.scopuseid_2-s2.0-85104275846-
dc.identifier.hkuros323453-
dc.identifier.volume28-
dc.identifier.issue3-
dc.identifier.spagearticle no. taab022-
dc.identifier.epagearticle no. taab022-
dc.publisher.placeUnited States-

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