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Article: High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis

TitleHigh Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
Authors
KeywordsESBL
Laribacter hongkongensis
AmpC beta-lactamase
Prevalence
Mechanism
Issue Date2021
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2021, v. 12, article no. 618894 How to Cite?
AbstractIn this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14 fish strains were tested ESBL-positive. However, ESBL genes, including SHV, TEM, CTX-M, GES, and PER, were not detected in all of these 28 L. hongkongensis isolates. No ESBL gene could be detected in either the complete genome of L. hongkongensis HLHK9 or the draft genome of PW3643. PCR and DNA sequencing revealed that all the 35 L. hongkongensis isolates (showing both ESBL-positive and ESBL-negative phenotypes) were positive for the ampC gene. When the AmpC deletion mutant, HLHK9ΔampC, was subject to the zone enhancement test, the difference of zone size between ceftazidime/clavulanate and ceftazidime was less than 5 mm. When boronic acid was added to the antibiotic disks, none of the 28 “ESBL-positive” isolates showed a ≥ 5 mm enhancement of inhibition zone size diameter between ceftazidime/clavulanate and ceftazidime and between cefotaxime/clavulanate and cefotaxime. A high prevalence (80%) of ESBL-positive phenotype is present in L. hongkongensis. Overall, our results suggested that the ESBL-positive phenotype in L. hongkongensis results from the expression of the intrinsic AmpC beta-lactamase. Confirmatory tests should be performed before issuing laboratory reports for L. hongkongensis isolates that are tested ESBL-positive by disk diffusion clavulanate inhibition test.
Persistent Identifierhttp://hdl.handle.net/10722/301403
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.065
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTeng, JLL-
dc.contributor.authorLuo, R-
dc.contributor.authorTang, BSF-
dc.contributor.authorFong, JYH-
dc.contributor.authorWang, L-
dc.contributor.authorJia, L-
dc.contributor.authorWong, CKS-
dc.contributor.authorChan, E-
dc.contributor.authorLeung, AWS-
dc.contributor.authorSiu, GKH-
dc.contributor.authorChiu, TH-
dc.contributor.authorFung, AMY-
dc.contributor.authorWu, AKL-
dc.contributor.authorYeung, ML-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2021-07-27T08:10:32Z-
dc.date.available2021-07-27T08:10:32Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Microbiology, 2021, v. 12, article no. 618894-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/301403-
dc.description.abstractIn this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14 fish strains were tested ESBL-positive. However, ESBL genes, including SHV, TEM, CTX-M, GES, and PER, were not detected in all of these 28 L. hongkongensis isolates. No ESBL gene could be detected in either the complete genome of L. hongkongensis HLHK9 or the draft genome of PW3643. PCR and DNA sequencing revealed that all the 35 L. hongkongensis isolates (showing both ESBL-positive and ESBL-negative phenotypes) were positive for the ampC gene. When the AmpC deletion mutant, HLHK9ΔampC, was subject to the zone enhancement test, the difference of zone size between ceftazidime/clavulanate and ceftazidime was less than 5 mm. When boronic acid was added to the antibiotic disks, none of the 28 “ESBL-positive” isolates showed a ≥ 5 mm enhancement of inhibition zone size diameter between ceftazidime/clavulanate and ceftazidime and between cefotaxime/clavulanate and cefotaxime. A high prevalence (80%) of ESBL-positive phenotype is present in L. hongkongensis. Overall, our results suggested that the ESBL-positive phenotype in L. hongkongensis results from the expression of the intrinsic AmpC beta-lactamase. Confirmatory tests should be performed before issuing laboratory reports for L. hongkongensis isolates that are tested ESBL-positive by disk diffusion clavulanate inhibition test.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectESBL-
dc.subjectLaribacter hongkongensis-
dc.subjectAmpC beta-lactamase-
dc.subjectPrevalence-
dc.subjectMechanism-
dc.titleHigh Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis-
dc.typeArticle-
dc.identifier.emailTeng, JLL: llteng@hku.hk-
dc.identifier.emailLuo, R: rbluo@cs.hku.hk-
dc.identifier.emailYeung, ML: pmlyeung@hku.hk-
dc.identifier.authorityTeng, JLL=rp00277-
dc.identifier.authorityLuo, R=rp02360-
dc.identifier.authorityYeung, ML=rp01402-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2021.618894-
dc.identifier.pmid33633706-
dc.identifier.pmcidPMC7902055-
dc.identifier.scopuseid_2-s2.0-85101238624-
dc.identifier.hkuros323501-
dc.identifier.volume12-
dc.identifier.spagearticle no. 618894-
dc.identifier.epagearticle no. 618894-
dc.identifier.isiWOS:000620560000001-
dc.publisher.placeSwitzerland-

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