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Article: NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes

TitleNanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes
Authors
KeywordsNanoSIMS imaging
White adipose tissue
Fatty acids
Nutrient uptake
Brown adipose tissue
Issue Date2018
Citation
Biochemical and Biophysical Research Communications, 2018, v. 504, n. 4, p. 899-902 How to Cite?
AbstractHeterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [2H]triglycerides (2H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined 13C enrichment in brown adipocytes after administering [13C]glucose or 13C-labeled mixed fatty acids by gastric gavage. The uptake of 2H-TRLs-derived lipids into brown adipocytes was heterogeneous, with 2H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in 13C enrichment in adjacent brown adipocytes after administering [13C]glucose or [13C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes.
Persistent Identifierhttp://hdl.handle.net/10722/301829
ISSN
2023 Impact Factor: 2.5
2023 SCImago Journal Rankings: 0.770
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHe, Cuiwen-
dc.contributor.authorHu, Xuchen-
dc.contributor.authorWeston, Thomas A.-
dc.contributor.authorJung, Rachel S.-
dc.contributor.authorHeizer, Patrick-
dc.contributor.authorTu, Yiping-
dc.contributor.authorEllison, Rochelle-
dc.contributor.authorMatsumoto, Ken-
dc.contributor.authorGerhardt, Holger-
dc.contributor.authorTontonoz, Peter-
dc.contributor.authorFong, Loren G.-
dc.contributor.authorYoung, Stephen G.-
dc.contributor.authorJiang, Haibo-
dc.date.accessioned2021-08-19T02:20:49Z-
dc.date.available2021-08-19T02:20:49Z-
dc.date.issued2018-
dc.identifier.citationBiochemical and Biophysical Research Communications, 2018, v. 504, n. 4, p. 899-902-
dc.identifier.issn0006-291X-
dc.identifier.urihttp://hdl.handle.net/10722/301829-
dc.description.abstractHeterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [2H]triglycerides (2H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined 13C enrichment in brown adipocytes after administering [13C]glucose or 13C-labeled mixed fatty acids by gastric gavage. The uptake of 2H-TRLs-derived lipids into brown adipocytes was heterogeneous, with 2H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in 13C enrichment in adjacent brown adipocytes after administering [13C]glucose or [13C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes.-
dc.languageeng-
dc.relation.ispartofBiochemical and Biophysical Research Communications-
dc.subjectNanoSIMS imaging-
dc.subjectWhite adipose tissue-
dc.subjectFatty acids-
dc.subjectNutrient uptake-
dc.subjectBrown adipose tissue-
dc.titleNanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.bbrc.2018.09.051-
dc.identifier.pmid30224066-
dc.identifier.pmcidPMC6476570-
dc.identifier.scopuseid_2-s2.0-85054436798-
dc.identifier.volume504-
dc.identifier.issue4-
dc.identifier.spage899-
dc.identifier.epage902-
dc.identifier.eissn1090-2104-
dc.identifier.isiWOS:000447681200042-

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