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- Publisher Website: 10.1371/journal.pbio.3000879
- Scopus: eid_2-s2.0-85098947679
- PMID: 33382684
- WOS: WOS:000605333000002
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Article: Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs
Title | Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs |
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Authors | |
Issue Date | 2020 |
Citation | PLoS Biology, 2020, v. 18, n. 12, article no. e3000879 How to Cite? |
Abstract | Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type–specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells. |
Persistent Identifier | http://hdl.handle.net/10722/301863 |
ISSN | 2023 Impact Factor: 7.8 2023 SCImago Journal Rankings: 3.822 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Fearns, Antony | - |
dc.contributor.author | Greenwood, Daniel J. | - |
dc.contributor.author | Rodgers, Angela | - |
dc.contributor.author | Jiang, Haibo | - |
dc.contributor.author | Gutierrez, Maximiliano G. | - |
dc.date.accessioned | 2021-08-19T02:20:53Z | - |
dc.date.available | 2021-08-19T02:20:53Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | PLoS Biology, 2020, v. 18, n. 12, article no. e3000879 | - |
dc.identifier.issn | 1544-9173 | - |
dc.identifier.uri | http://hdl.handle.net/10722/301863 | - |
dc.description.abstract | Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type–specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells. | - |
dc.language | eng | - |
dc.relation.ispartof | PLoS Biology | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pbio.3000879 | - |
dc.identifier.pmid | 33382684 | - |
dc.identifier.pmcid | PMC7810513 | - |
dc.identifier.scopus | eid_2-s2.0-85098947679 | - |
dc.identifier.hkuros | 327221 | - |
dc.identifier.volume | 18 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | article no. e3000879 | - |
dc.identifier.epage | article no. e3000879 | - |
dc.identifier.eissn | 1545-7885 | - |
dc.identifier.isi | WOS:000605333000002 | - |