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Article: Gut lumen formation defect can cause intestinal atresia: evidence from histological studies of human embryos and intestinal atresia septum
Title | Gut lumen formation defect can cause intestinal atresia: evidence from histological studies of human embryos and intestinal atresia septum |
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Authors | |
Keywords | Vacuoles recanalization intestinal atresia embryos |
Issue Date | 2021 |
Publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=DOH |
Citation | Journal of Developmental Origins of Health and Disease, 2021, Epub 2021-04-12 How to Cite? |
Abstract | Intestinal atresia (IA), a common cause of neonatal intestinal obstruction, is a developmental defect, which disrupts the luminal continuity of the intestine. Here, we investigated (i) the process of lumen formation in human embryos; and (ii) how a defective lumen formation led to IA. We performed histological and histochemical study on 6–10 gestation week human embryos and on IA septal regions. To investigate the topology of embryonic intestine development, we conducted 3D reconstruction. We showed that a 6–7th gestation week embryonic gut has no lumen, but filled with mesenchyme cells and vacuoles of a monolayer of epithelial cells. A narrow gut lumen was formed by gestation week-9, the gut was filled with numerous vacuoles of different sizes, some vacuoles were merging with the developing embryonic gut wall. At gestation week-10, a prominent lumen was developed, only few vacuoles were present and were merging with the intestine wall. At IA septal regions, vacuoles were located in the submucous layer, covered by a single layer of epithelium without glandular structure, and surrounded with fibrous tissue. The mucosal epithelium was developed with lamina propria and basement membrane, but the submucosa and the longitudinal smooth muscle layers were not properly developed. Hence, the vacuoles in IA septum could represent a remnant of vacuoles of embryonic gut. In conclusion, the fusion of vacuoles with the developing intestine wall associates with the disappearance of vacuoles and gut lumen formation in human embryos, and perturbation of these developmental events could lead to IA. |
Persistent Identifier | http://hdl.handle.net/10722/302123 |
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.659 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liu, X | - |
dc.contributor.author | Hao, PY | - |
dc.contributor.author | Lui, VCH | - |
dc.contributor.author | Xie, X | - |
dc.contributor.author | Li, Y | - |
dc.contributor.author | Song, Y | - |
dc.contributor.author | Li, L | - |
dc.contributor.author | Jin, ZW | - |
dc.date.accessioned | 2021-08-21T03:31:55Z | - |
dc.date.available | 2021-08-21T03:31:55Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Journal of Developmental Origins of Health and Disease, 2021, Epub 2021-04-12 | - |
dc.identifier.issn | 2040-1744 | - |
dc.identifier.uri | http://hdl.handle.net/10722/302123 | - |
dc.description.abstract | Intestinal atresia (IA), a common cause of neonatal intestinal obstruction, is a developmental defect, which disrupts the luminal continuity of the intestine. Here, we investigated (i) the process of lumen formation in human embryos; and (ii) how a defective lumen formation led to IA. We performed histological and histochemical study on 6–10 gestation week human embryos and on IA septal regions. To investigate the topology of embryonic intestine development, we conducted 3D reconstruction. We showed that a 6–7th gestation week embryonic gut has no lumen, but filled with mesenchyme cells and vacuoles of a monolayer of epithelial cells. A narrow gut lumen was formed by gestation week-9, the gut was filled with numerous vacuoles of different sizes, some vacuoles were merging with the developing embryonic gut wall. At gestation week-10, a prominent lumen was developed, only few vacuoles were present and were merging with the intestine wall. At IA septal regions, vacuoles were located in the submucous layer, covered by a single layer of epithelium without glandular structure, and surrounded with fibrous tissue. The mucosal epithelium was developed with lamina propria and basement membrane, but the submucosa and the longitudinal smooth muscle layers were not properly developed. Hence, the vacuoles in IA septum could represent a remnant of vacuoles of embryonic gut. In conclusion, the fusion of vacuoles with the developing intestine wall associates with the disappearance of vacuoles and gut lumen formation in human embryos, and perturbation of these developmental events could lead to IA. | - |
dc.language | eng | - |
dc.publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=DOH | - |
dc.relation.ispartof | Journal of Developmental Origins of Health and Disease | - |
dc.rights | Journal of Developmental Origins of Health and Disease. Copyright © Cambridge University Press. | - |
dc.rights | This article has been published in a revised form in [Journal] [http://doi.org/XXX]. This version is free to view and download for private research and study only. Not for re-distribution, re-sale or use in derivative works. © copyright holder. | - |
dc.subject | Vacuoles | - |
dc.subject | recanalization | - |
dc.subject | intestinal atresia | - |
dc.subject | embryos | - |
dc.title | Gut lumen formation defect can cause intestinal atresia: evidence from histological studies of human embryos and intestinal atresia septum | - |
dc.type | Article | - |
dc.identifier.email | Lui, VCH: vchlui@hku.hk | - |
dc.identifier.authority | Lui, VCH=rp00363 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1017/S2040174421000088 | - |
dc.identifier.scopus | eid_2-s2.0-85104049250 | - |
dc.identifier.hkuros | 324616 | - |
dc.identifier.volume | Epub 2021-04-12 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 7 | - |
dc.identifier.isi | WOS:000760346200008 | - |
dc.publisher.place | United Kingdom | - |