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Article: The anti-scarring role of Lycium barbarum polysaccharide on cornea epithelial-stromal injury

TitleThe anti-scarring role of Lycium barbarum polysaccharide on cornea epithelial-stromal injury
Authors
KeywordsFibroblasts
Myofibroblasts
Stromal fibrosis
Transforming growth factor-beta 1
3D culturing
Issue Date2021
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer
Citation
Experimental Eye Research, 2021, v. 211, article no. 108747 How to Cite?
AbstractPurpose: Cornea epithelial-stromal scarring is related to the differentiation of fibroblasts into opaque myofibroblasts. Our study aims to assess the effectiveness of Lycium barbarum polysaccharide (LBP) solution as a pre-treatment in minimizing corneal scarring. Methods: Human corneal fibroblasts were cultured in a three-dimensional collagen type I-based hydrogel in an eye-on-a-chip model. Fibroblasts were pre-treated with 2 mg/mL LBP for 24 h, followed by another 24-h incubation with 10 ng/mL transforming growth factor-beta 1 (TGF-β1) to induce relevant physiological events after stromal injury. Intracellular pro-fibrotic proteins, extracellular matrix proteins, and pro-inflammatory cytokines that involved in fibrosis, were assessed using immunocytochemistry and enzyme-linked immunosorbent assays. Results: Compared to the positive control TGF-β1 group, LBP pre-treated cells had a significantly lower expression of alpha-smooth muscle actin, marker of myofibroblasts, vimentin (p < 0.05), and also extracellular matrix proteins both collagen type II and type III (p < 0.05) that can be found in scar tissues. Moreover, LBP pre-treated cells had a significantly lower secretion of pro-inflammatory cytokines interleukin-6 and interleukin-8 (p < 0.05). The cell-laden hydrogel contraction and stiffness showed no significant difference between LBP pre-treatment and control groups. Fibroblasts pretreated with LBP as well had reduced angiogenic factors expression and suppression of undesired proliferation (p < 0.05). Conclusion: Our results showed that LBP reduced both pro-fibrotic proteins and pro-inflammatory cytokines on corneal injury in vitro. We suggest that LBP, as a natural Traditional Chinese Medicine, may potentially be a novel topical pre-treatment option prior to corneal refractive surgeries with an improved prognosis.
Persistent Identifierhttp://hdl.handle.net/10722/302440
ISSN
2023 Impact Factor: 3.0
2023 SCImago Journal Rankings: 1.020
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, HL-
dc.contributor.authorHung, LT-
dc.contributor.authorKwok, SS-
dc.contributor.authorBu, Y-
dc.contributor.authorLin, Y-
dc.contributor.authorShum, HC-
dc.contributor.authorWang, H-
dc.contributor.authorLo, ACY-
dc.contributor.authorYam, GHF-
dc.contributor.authorJhanji, V-
dc.contributor.authorShih, KC-
dc.contributor.authorChan, YK-
dc.date.accessioned2021-09-06T03:32:19Z-
dc.date.available2021-09-06T03:32:19Z-
dc.date.issued2021-
dc.identifier.citationExperimental Eye Research, 2021, v. 211, article no. 108747-
dc.identifier.issn0014-4835-
dc.identifier.urihttp://hdl.handle.net/10722/302440-
dc.description.abstractPurpose: Cornea epithelial-stromal scarring is related to the differentiation of fibroblasts into opaque myofibroblasts. Our study aims to assess the effectiveness of Lycium barbarum polysaccharide (LBP) solution as a pre-treatment in minimizing corneal scarring. Methods: Human corneal fibroblasts were cultured in a three-dimensional collagen type I-based hydrogel in an eye-on-a-chip model. Fibroblasts were pre-treated with 2 mg/mL LBP for 24 h, followed by another 24-h incubation with 10 ng/mL transforming growth factor-beta 1 (TGF-β1) to induce relevant physiological events after stromal injury. Intracellular pro-fibrotic proteins, extracellular matrix proteins, and pro-inflammatory cytokines that involved in fibrosis, were assessed using immunocytochemistry and enzyme-linked immunosorbent assays. Results: Compared to the positive control TGF-β1 group, LBP pre-treated cells had a significantly lower expression of alpha-smooth muscle actin, marker of myofibroblasts, vimentin (p < 0.05), and also extracellular matrix proteins both collagen type II and type III (p < 0.05) that can be found in scar tissues. Moreover, LBP pre-treated cells had a significantly lower secretion of pro-inflammatory cytokines interleukin-6 and interleukin-8 (p < 0.05). The cell-laden hydrogel contraction and stiffness showed no significant difference between LBP pre-treatment and control groups. Fibroblasts pretreated with LBP as well had reduced angiogenic factors expression and suppression of undesired proliferation (p < 0.05). Conclusion: Our results showed that LBP reduced both pro-fibrotic proteins and pro-inflammatory cytokines on corneal injury in vitro. We suggest that LBP, as a natural Traditional Chinese Medicine, may potentially be a novel topical pre-treatment option prior to corneal refractive surgeries with an improved prognosis.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer-
dc.relation.ispartofExperimental Eye Research-
dc.subjectFibroblasts-
dc.subjectMyofibroblasts-
dc.subjectStromal fibrosis-
dc.subjectTransforming growth factor-beta 1-
dc.subject3D culturing-
dc.titleThe anti-scarring role of Lycium barbarum polysaccharide on cornea epithelial-stromal injury-
dc.typeArticle-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.emailLo, ACY: amylo@hku.hk-
dc.identifier.emailShih, KC: kcshih@hku.hk-
dc.identifier.emailChan, YK: josephyk@connect.hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.identifier.authorityShum, HC=rp01439-
dc.identifier.authorityLo, ACY=rp00425-
dc.identifier.authorityShih, KC=rp01374-
dc.identifier.authorityChan, YK=rp02536-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exer.2021.108747-
dc.identifier.pmid34450184-
dc.identifier.scopuseid_2-s2.0-85113629816-
dc.identifier.hkuros324704-
dc.identifier.volume211-
dc.identifier.spagearticle no. 108747-
dc.identifier.epagearticle no. 108747-
dc.identifier.isiWOS:000697418000004-
dc.publisher.placeUnited Kingdom-

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