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Article: Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine

TitleInduction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine
Authors
Issue Date2021
PublisherAmerican Association for the Advancement of Science: Science Advances. The Journal's web site is located at http://www.scienceadvances.org/
Citation
Science Advances, 2021, v. 7 n. 3, p. article no. eabd1929 How to Cite?
AbstractTerminally differentiated murine osteocytes and adipocytes can be reprogrammed using platelet-derived growth factor-AB and 5-azacytidine into multipotent stem cells with stromal cell characteristics. We have now optimized culture conditions to reprogram human adipocytes into induced multipotent stem (iMS) cells and characterized their molecular and functional properties. Although the basal transcriptomes of adipocyte-derived iMS cells and adipose tissue-derived mesenchymal stem cells were similar, there were changes in histone modifications and CpG methylation at cis-regulatory regions consistent with an epigenetic landscape that was primed for tissue development and differentiation. In a non-specific tissue injury xenograft model, iMS cells contributed directly to muscle, bone, cartilage, and blood vessels, with no evidence of teratogenic potential. In a cardiotoxin muscle injury model, iMS cells contributed specifically to satellite cells and myofibers without ectopic tissue formation. Together, human adipocyte-derived iMS cells regenerate tissues in a context-dependent manner without ectopic or neoplastic growth.
Persistent Identifierhttp://hdl.handle.net/10722/304194
ISSN
2023 Impact Factor: 11.7
2023 SCImago Journal Rankings: 4.483
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeola, A-
dc.contributor.authorSubramanian, S-
dc.contributor.authorOliver, RA-
dc.contributor.authorLucas, CA-
dc.contributor.authorThoms, JAI-
dc.contributor.authorYan, F-
dc.contributor.authorOlivier, J-
dc.contributor.authorChacon, D-
dc.contributor.authorTursky, ML-
dc.contributor.authorSrivastava, P-
dc.contributor.authorPotas, JR-
dc.contributor.authorHung, T-
dc.contributor.authorPower, C-
dc.contributor.authorHardy, P-
dc.contributor.authorMa, DD-
dc.contributor.authorKilian, KA-
dc.contributor.authorMcCarroll, J-
dc.contributor.authorKavallaris, M-
dc.contributor.authorHesson, LB-
dc.contributor.authorBeck, D-
dc.contributor.authorCurtis, DJ-
dc.contributor.authorWong, JWH-
dc.contributor.authorHardeman, EC-
dc.contributor.authorWalsh, WR-
dc.contributor.authorMobbs, R-
dc.contributor.authorChandrakanthan, V-
dc.contributor.authorPimanda, JE-
dc.date.accessioned2021-09-23T08:56:33Z-
dc.date.available2021-09-23T08:56:33Z-
dc.date.issued2021-
dc.identifier.citationScience Advances, 2021, v. 7 n. 3, p. article no. eabd1929-
dc.identifier.issn2375-2548-
dc.identifier.urihttp://hdl.handle.net/10722/304194-
dc.description.abstractTerminally differentiated murine osteocytes and adipocytes can be reprogrammed using platelet-derived growth factor-AB and 5-azacytidine into multipotent stem cells with stromal cell characteristics. We have now optimized culture conditions to reprogram human adipocytes into induced multipotent stem (iMS) cells and characterized their molecular and functional properties. Although the basal transcriptomes of adipocyte-derived iMS cells and adipose tissue-derived mesenchymal stem cells were similar, there were changes in histone modifications and CpG methylation at cis-regulatory regions consistent with an epigenetic landscape that was primed for tissue development and differentiation. In a non-specific tissue injury xenograft model, iMS cells contributed directly to muscle, bone, cartilage, and blood vessels, with no evidence of teratogenic potential. In a cardiotoxin muscle injury model, iMS cells contributed specifically to satellite cells and myofibers without ectopic tissue formation. Together, human adipocyte-derived iMS cells regenerate tissues in a context-dependent manner without ectopic or neoplastic growth.-
dc.languageeng-
dc.publisherAmerican Association for the Advancement of Science: Science Advances. The Journal's web site is located at http://www.scienceadvances.org/-
dc.relation.ispartofScience Advances-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleInduction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine-
dc.typeArticle-
dc.identifier.emailWong, JWH: jwhwong@hku.hk-
dc.identifier.authorityWong, JWH=rp02363-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1126/sciadv.abd1929-
dc.identifier.pmid33523875-
dc.identifier.pmcidPMC7806226-
dc.identifier.scopuseid_2-s2.0-85099981894-
dc.identifier.hkuros325653-
dc.identifier.volume7-
dc.identifier.issue3-
dc.identifier.spagearticle no. eabd1929-
dc.identifier.epagearticle no. eabd1929-
dc.identifier.isiWOS:000608481000019-
dc.publisher.placeUnited States-

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