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- Publisher Website: 10.1016/j.cell.2021.02.053
- Scopus: eid_2-s2.0-85102449487
- PMID: 33713620
- WOS: WOS:000640387300026
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Article: Soluble ACE2-mediated cell entry of SARS-CoV-2 via interaction with proteins related to the renin-angiotensin system
Title | Soluble ACE2-mediated cell entry of SARS-CoV-2 via interaction with proteins related to the renin-angiotensin system |
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Authors | |
Keywords | SARS-CoV-2 RNAi ACE2 sACE2 COVID-19 |
Issue Date | 2021 |
Publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell |
Citation | Cell, 2021, v. 184 n. 8, p. 2212-2228.e12 How to Cite? |
Abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause acute respiratory disease and multiorgan failure. Finding human host factors that are essential for SARS-CoV-2 infection could facilitate the formulation of treatment strategies. Using a human kidney cell line—HK-2—that is highly susceptible to SARS-CoV-2, we performed a genome-wide RNAi screen and identified virus dependency factors (VDFs), which play regulatory roles in biological pathways linked to clinical manifestations of SARS-CoV-2 infection. We found a role for a secretory form of SARS-CoV-2 receptor, soluble angiotensin converting enzyme 2 (sACE2), in SARS-CoV-2 infection. Further investigation revealed that SARS-CoV-2 exploits receptor-mediated endocytosis through interaction between its spike with sACE2 or sACE2-vasopressin via AT1 or AVPR1B, respectively. Our identification of VDFs and the regulatory effect of sACE2 on SARS-CoV-2 infection shed insight into pathogenesis and cell entry mechanisms of SARS-CoV-2 as well as potential treatment strategies for COVID-19. |
Persistent Identifier | http://hdl.handle.net/10722/304621 |
ISSN | 2023 Impact Factor: 45.5 2023 SCImago Journal Rankings: 24.342 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yeung, ML | - |
dc.contributor.author | Teng, JLL | - |
dc.contributor.author | Jia, L | - |
dc.contributor.author | ZHANG, C | - |
dc.contributor.author | Huang, C | - |
dc.contributor.author | Cai, JP | - |
dc.contributor.author | Zhou, R | - |
dc.contributor.author | Chan, KH | - |
dc.contributor.author | Zhao, H | - |
dc.contributor.author | Zhu, L | - |
dc.contributor.author | Siu, KL | - |
dc.contributor.author | Fung, SY | - |
dc.contributor.author | Yung, S | - |
dc.contributor.author | Chan, TM | - |
dc.contributor.author | To, KKW | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Cai, Z | - |
dc.contributor.author | Lau, SKP | - |
dc.contributor.author | Chen, Z | - |
dc.contributor.author | Jin, DY | - |
dc.contributor.author | Woo, PCY | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2021-10-05T02:32:45Z | - |
dc.date.available | 2021-10-05T02:32:45Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Cell, 2021, v. 184 n. 8, p. 2212-2228.e12 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10722/304621 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause acute respiratory disease and multiorgan failure. Finding human host factors that are essential for SARS-CoV-2 infection could facilitate the formulation of treatment strategies. Using a human kidney cell line—HK-2—that is highly susceptible to SARS-CoV-2, we performed a genome-wide RNAi screen and identified virus dependency factors (VDFs), which play regulatory roles in biological pathways linked to clinical manifestations of SARS-CoV-2 infection. We found a role for a secretory form of SARS-CoV-2 receptor, soluble angiotensin converting enzyme 2 (sACE2), in SARS-CoV-2 infection. Further investigation revealed that SARS-CoV-2 exploits receptor-mediated endocytosis through interaction between its spike with sACE2 or sACE2-vasopressin via AT1 or AVPR1B, respectively. Our identification of VDFs and the regulatory effect of sACE2 on SARS-CoV-2 infection shed insight into pathogenesis and cell entry mechanisms of SARS-CoV-2 as well as potential treatment strategies for COVID-19. | - |
dc.language | eng | - |
dc.publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell | - |
dc.relation.ispartof | Cell | - |
dc.subject | SARS-CoV-2 | - |
dc.subject | RNAi | - |
dc.subject | ACE2 | - |
dc.subject | sACE2 | - |
dc.subject | COVID-19 | - |
dc.title | Soluble ACE2-mediated cell entry of SARS-CoV-2 via interaction with proteins related to the renin-angiotensin system | - |
dc.type | Article | - |
dc.identifier.email | Yeung, ML: pmlyeung@hku.hk | - |
dc.identifier.email | Teng, JLL: llteng@hku.hk | - |
dc.identifier.email | Jia, L: justin86@hku.hk | - |
dc.identifier.email | Huang, C: chengxh@hku.hk | - |
dc.identifier.email | Cai, JP: caijuice@hku.hk | - |
dc.identifier.email | Zhou, R: zhourh@hku.hk | - |
dc.identifier.email | Chan, KH: chankh2@hkucc.hku.hk | - |
dc.identifier.email | Zhao, H: hjzhao13@hku.hk | - |
dc.identifier.email | Siu, KL: sklsfx@hkucc.hku.hk | - |
dc.identifier.email | Fung, SY: kittyfsy@connect.hku.hk | - |
dc.identifier.email | Yung, S: ssyyung@hku.hk | - |
dc.identifier.email | To, KKW: kelvinto@hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Chen, Z: zchenai@hku.hk | - |
dc.identifier.email | Jin, DY: dyjin@hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Yeung, ML=rp01402 | - |
dc.identifier.authority | Teng, JLL=rp00277 | - |
dc.identifier.authority | Chan, KH=rp01921 | - |
dc.identifier.authority | Zhao, H=rp02653 | - |
dc.identifier.authority | Yung, S=rp00455 | - |
dc.identifier.authority | To, KKW=rp01384 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Lau, SKP=rp00486 | - |
dc.identifier.authority | Chen, Z=rp00243 | - |
dc.identifier.authority | Jin, DY=rp00452 | - |
dc.identifier.authority | Woo, PCY=rp00430 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/j.cell.2021.02.053 | - |
dc.identifier.pmid | 33713620 | - |
dc.identifier.pmcid | PMC7923941 | - |
dc.identifier.scopus | eid_2-s2.0-85102449487 | - |
dc.identifier.hkuros | 326083 | - |
dc.identifier.volume | 184 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 2212 | - |
dc.identifier.epage | 2228.e12 | - |
dc.identifier.isi | WOS:000640387300026 | - |
dc.publisher.place | United States | - |