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Article: Humanizing the Yeast Origin Recognition Complex

TitleHumanizing the Yeast Origin Recognition Complex
Authors
Issue Date2021
PublisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2021, v. 12 n. 1, p. article no. 33 How to Cite?
AbstractThe Origin Recognition Complex (ORC) is an evolutionarily conserved six-subunit protein complex that binds specific sites at many locations to coordinately replicate the entire eukaryote genome. Though highly conserved in structure, ORC’s selectivity for replication origins has diverged tremendously between yeasts and humans to adapt to vastly different life cycles. In this work, we demonstrate that the selectivity determinant of ORC for DNA binding lies in a 19-amino acid insertion helix in the Orc4 subunit, which is present in yeast but absent in human. Removal of this motif from Orc4 transforms the yeast ORC, which selects origins based on base-specific binding at defined locations, into one whose selectivity is dictated by chromatin landscape and afforded with plasticity, as reported for human. Notably, the altered yeast ORC has acquired an affinity for regions near transcriptional start sites (TSSs), which the human ORC also favors
Persistent Identifierhttp://hdl.handle.net/10722/304835
ISSN
2023 Impact Factor: 14.7
2023 SCImago Journal Rankings: 4.887
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, CSK-
dc.contributor.authorCheung, MF-
dc.contributor.authorLi, J-
dc.contributor.authorZhao, Y-
dc.contributor.authorHo, V-
dc.contributor.authorRohs, R-
dc.contributor.authorZhai, Y-
dc.contributor.authorLeung, D-
dc.contributor.authorTye, BK-
dc.contributor.authorLam, WH-
dc.date.accessioned2021-10-05T02:35:53Z-
dc.date.available2021-10-05T02:35:53Z-
dc.date.issued2021-
dc.identifier.citationNature Communications, 2021, v. 12 n. 1, p. article no. 33-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/304835-
dc.description.abstractThe Origin Recognition Complex (ORC) is an evolutionarily conserved six-subunit protein complex that binds specific sites at many locations to coordinately replicate the entire eukaryote genome. Though highly conserved in structure, ORC’s selectivity for replication origins has diverged tremendously between yeasts and humans to adapt to vastly different life cycles. In this work, we demonstrate that the selectivity determinant of ORC for DNA binding lies in a 19-amino acid insertion helix in the Orc4 subunit, which is present in yeast but absent in human. Removal of this motif from Orc4 transforms the yeast ORC, which selects origins based on base-specific binding at defined locations, into one whose selectivity is dictated by chromatin landscape and afforded with plasticity, as reported for human. Notably, the altered yeast ORC has acquired an affinity for regions near transcriptional start sites (TSSs), which the human ORC also favors-
dc.languageeng-
dc.publisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsNature Communications. Copyright © Nature Research: Fully open access journals.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleHumanizing the Yeast Origin Recognition Complex-
dc.typeArticle-
dc.identifier.emailLee, CSK: leclare@hku.hk-
dc.identifier.emailZhai, Y: zhai@hku.hk-
dc.identifier.authorityZhai, Y=rp02398-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-020-20277-y-
dc.identifier.pmid33397927-
dc.identifier.pmcidPMC7782691-
dc.identifier.scopuseid_2-s2.0-85098628175-
dc.identifier.hkuros325830-
dc.identifier.volume12-
dc.identifier.issue1-
dc.identifier.spagearticle no. 33-
dc.identifier.epagearticle no. 33-
dc.identifier.isiWOS:000665627200009-
dc.publisher.placeUnited Kingdom-

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