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Article: Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater

TitleMetagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
Authors
Keywordsantibiotic resistance
antibiotic resistance genes
biocide/metal resistance genes
hospital wastewater
municipal wastewater
Issue Date2021
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2021, v. 12, p. article no. 712843 How to Cite?
AbstractAlongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains poorly described. In this study, we used metagenomic sequencing to characterize ARGs and biocide/metal resistance genes (BMRGs) in four wastewater treatment plants (WWTPs) in Jaipur City of India. We observed a significantly lower richness and abundance of ARGs in the influent of a WWTP exclusively receiving hospital wastewater when compared to other three WWTPs involving municipal wastewater treatment. Several tetracycline and macrolide-lincosamide-streptogramin resistance genes were enriched in influents of these three municipal wastewater-related treatment plants, whereas hospital wastewater had a higher abundance of genes conferring resistance to disinfectant-related compounds such as synergize and wex-cide-128, reflecting the patterns of antibiotic/disinfectant use. Of note, in the wastewater system with more chemicals, there was a strong correlation between the numbers of ARGs and BMRGs potentially harbored by common hosts. Our study highlights significant influxes of ARGs from non-hospital sources in Jaipur City, and thus more attention should be paid on the emergence of ARGs in general communities.
Persistent Identifierhttp://hdl.handle.net/10722/304886
ISSN
2020 Impact Factor: 5.64
2020 SCImago Journal Rankings: 1.701
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, D-
dc.contributor.authorPENG, Y-
dc.contributor.authorCHAN, CL-
dc.contributor.authorOn, H-
dc.contributor.authorWAI, HKF-
dc.contributor.authorShekhawat, SS-
dc.contributor.authorGupta, AB-
dc.contributor.authorVarshney, AK-
dc.contributor.authorChuanchuen, R-
dc.contributor.authorZhou, X-
dc.contributor.authorXia, Y-
dc.contributor.authorLIANG, S-
dc.contributor.authorFukuda, K-
dc.contributor.authorMedicherla, KM-
dc.contributor.authorTun, HM-
dc.date.accessioned2021-10-05T02:36:37Z-
dc.date.available2021-10-05T02:36:37Z-
dc.date.issued2021-
dc.identifier.citationFrontiers in Microbiology, 2021, v. 12, p. article no. 712843-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/304886-
dc.description.abstractAlongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains poorly described. In this study, we used metagenomic sequencing to characterize ARGs and biocide/metal resistance genes (BMRGs) in four wastewater treatment plants (WWTPs) in Jaipur City of India. We observed a significantly lower richness and abundance of ARGs in the influent of a WWTP exclusively receiving hospital wastewater when compared to other three WWTPs involving municipal wastewater treatment. Several tetracycline and macrolide-lincosamide-streptogramin resistance genes were enriched in influents of these three municipal wastewater-related treatment plants, whereas hospital wastewater had a higher abundance of genes conferring resistance to disinfectant-related compounds such as synergize and wex-cide-128, reflecting the patterns of antibiotic/disinfectant use. Of note, in the wastewater system with more chemicals, there was a strong correlation between the numbers of ARGs and BMRGs potentially harbored by common hosts. Our study highlights significant influxes of ARGs from non-hospital sources in Jaipur City, and thus more attention should be paid on the emergence of ARGs in general communities.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectantibiotic resistance-
dc.subjectantibiotic resistance genes-
dc.subjectbiocide/metal resistance genes-
dc.subjecthospital wastewater-
dc.subjectmunicipal wastewater-
dc.titleMetagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater-
dc.typeArticle-
dc.identifier.emailOn, H: hildaon@hku.hk-
dc.identifier.emailFukuda, K: kfukuda@hku.hk-
dc.identifier.emailTun, HM: heinmtun@hku.hk-
dc.identifier.authorityFukuda, K=rp02247-
dc.identifier.authorityTun, HM=rp02389-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2021.712843-
dc.identifier.pmid34526976-
dc.identifier.pmcidPMC8435860-
dc.identifier.scopuseid_2-s2.0-85114837748-
dc.identifier.hkuros326423-
dc.identifier.volume12-
dc.identifier.spagearticle no. 712843-
dc.identifier.epagearticle no. 712843-
dc.publisher.placeSwitzerland-

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